Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,044,431 T→C V257A (GTG→GCG)  bglA → 6‑phospho‑beta‑glucosidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,044,4310TC100.0% 81.2 / NA 24V257A (GTG→GCG) bglA6‑phospho‑beta‑glucosidase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/11);  total (13/11)

CTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGG  >  NC_000913/3044304‑3044557
                                                                                                                               |                                                                                                                              
ctgcATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATACTTACTCATGTTACACGGACgatgcgatgt                                                                                                                           >  1:164841/1‑133 (MQ=255)
ctgcATCACAAGTTTCTCGCCAGCTCCCAGGCGGTGAAAGCCGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGTTGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACgatgcgatgt                                                                                                                           <  2:224683/133‑1 (MQ=255)
 tgcATCACGAGTTTGGCGACAGCGCCCTGGGGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGCGATGtt                                                                                                                          <  1:215580/133‑1 (MQ=255)
 tgcATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGCGATGtt                                                                                                                          >  2:120915/1‑133 (MQ=255)
 tgcATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGCGATGtt                                                                                                                          >  1:276191/1‑133 (MQ=255)
 tgcATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTACCGCTCTAGCCTGACTCCTGTAACCCGGACgttgcgatgct                                                                                                                          >  2:238037/1‑131 (MQ=255)
   cATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGCGATGTTCg                                                                                                                        >  1:289222/1‑133 (MQ=255)
      cACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGCGATGTTCGCTc                                                                                                                     <  1:120915/133‑1 (MQ=255)
            tttGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGCGCTGTTAGATCAGGAgg                                                                                                               >  2:220748/1‑132 (MQ=255)
                           cTGGCGGTGAAAGCTTCGCGTCGCATTAACTCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGCGATGTTCGCTCAGGAGTCGATGCGCGAACGCt                                                                                                <  1:238037/133‑1 (MQ=255)
                             ggCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGCGATGTTCGCTCATGAGTCGATGCGCGAACGCTAc                                                                                              <  1:85271/133‑1 (MQ=255)
                             ggCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGCGATGTTCGCTCAGGAGTCGATGCGCGAACGCTAc                                                                                              <  2:461408/133‑1 (MQ=255)
                               cGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGCGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGt                                                                                            <  2:34858/133‑1 (MQ=255)
                                                       aCCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGCGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCgg                                                                    <  1:325772/133‑1 (MQ=255)
                                                         ccGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGCGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCt                                                                  <  1:413025/133‑1 (MQ=255)
                                                           ggAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGCGATGTTCGCTCAGGAGTCGGTGCGCGAACGCTTCGTCTTTGCCGATGTGCAGCTAGGCGGCTAt                                                                <  2:495797/133‑1 (MQ=255)
                                                             agaTGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACTCGGACGATGCGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTa                                                              >  2:499278/1‑133 (MQ=255)
                                                                     gTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGCGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCt                                                      <  1:123421/133‑1 (MQ=255)
                                                                             tATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGCGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTg                                              >  1:446115/1‑133 (MQ=255)
                                                                                              cGCTCTATCCTTACTCCTGTAACCCGGACGATGCGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTcg                             >  1:419324/1‑133 (MQ=255)
                                                                                                          aCTCCTGTAACCCGGACGATGCGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACAt                 >  1:430860/1‑133 (MQ=255)
                                                                                                          aCTCCTGTAACCCGGACGATGCGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACAt                 <  2:289222/133‑1 (MQ=255)
                                                                                                          aCTCCTGTAACCCGGACGATCCGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACAt                 >  1:326018/1‑133 (MQ=255)
                                                                                                               tGTAACCCGGACGATGCGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGAAGCGTCGCGGATTCAACATCaaaa            >  2:180233/1‑133 (MQ=255)
                                                                                                               tGTAACCCGGACGATGCGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACAAGTGGGACCGTGGCTGATTTAACATCaaaa            >  1:178829/1‑133 (MQ=255)
                                                                                                                   aCCCGGACGATGCGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGa        >  1:421264/1‑133 (MQ=255)
                                                                                                                        gACGATGCGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGTGTCGCGGATTTAACGTCAAAACGGAAGACg   >  1:225682/1‑133 (MQ=255)
                                                                                                                        gACGATGCGATGTTCGCTCAGGAGCCGATGCGCGAACGCTACGTCTTTACCGATGCGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGAGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACg   >  2:473348/1‑133 (MQ=255)
                                                                                                                         aCGATGCGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACgg  <  2:276191/133‑1 (MQ=255)
                                                                                                                               |                                                                                                                              
CTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGATGTGCAGCTACGCGGCTATTACCCGTCCTATGTGTTGAACGAGTGGGAGCGTCGCGGATTTAACATCAAAATGGAAGACGG  >  NC_000913/3044304‑3044557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑