Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,076,876	T→C	Y194C (TAC→TGC)	yggP ←	putative zinc‑binding dehydrogenase YggP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,076,876	0	T	C	100.0%	108.5 / NA	32	Y194C (TAC→TGC)	yggP	putative zinc‑binding dehydrogenase YggP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/17);  total (15/17)

ACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAA  >  NC_000913/3076747‑3077005                                                                                                                                  |                                                                                                                                  acaTACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGCAAt                                                                                                                                >  1:300390/1‑133 (MQ=255)     acacCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGCAATCGAt                                                                                                                            >  1:331474/1‑133 (MQ=255)      cacCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGCAATCGATa                                                                                                                           >  2:502589/1‑133 (MQ=255)         cAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCAGCGACGGTTGTAGGCCACCGTTAATCGCGCGATCGATAgcg                                                                                                                        >  2:150555/1‑133 (MQ=255)           gCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTAAGCCGCCGATAAGGGGGCAATCGATAGCGcc                                                                                                                      >  1:137906/1‑133 (MQ=255)            cTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGCAATCGATAGCGCCa                                                                                                                     >  1:481403/1‑133 (MQ=255)            cTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGCAATCGATAGCGCCa                                                                                                                     >  1:73505/1‑133 (MQ=255)             tCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGCAATCGATAGCGCCaa                                                                                                                    >  1:280008/1‑133 (MQ=255)                 tGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGTCGCAATCGATAGCGCCAATGcc                                                                                                                >  1:26549/1‑133 (MQ=255)                      ttACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTATTGGCGCAGTCGGTAGCGCCAAGGCCCATCg                                                                                                           >  1:187437/1‑133 (MQ=255)                      ttACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGCAATCGATAGCGCCAATGCCTATCg                                                                                                           >  1:268471/1‑133 (MQ=255)                                      ccACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGCAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCcag                                                                                           <  1:425508/133‑1 (MQ=255)                                                               cgcCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGCAATCGATAGCGCCAATGCCCATCGGACCTGCACATGCCAGCACTGCGATGTTGCCGGCAGGTTTg                                                                  >  1:148443/1‑133 (MQ=255)                                                               cgcCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGCAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTg                                                                  <  1:23908/133‑1 (MQ=255)                                                                gcCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGCAATTGATAGCGCCAATGCCCATCGGACCTGCACTCGCCAGCAGTGCAATATTGCCGCCAGGTTTGa                                                                 <  2:320525/133‑1 (MQ=255)                                                                 ccAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGCAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGAc                                                                >  1:316619/1‑133 (MQ=255)                                                                          ggccgtcgATATCGCCAACCCCCCCCCGCGACGGTTGTAGGCCGCCGTTAATGGCGCAATCGATAGCGCCAATGCCCGTCGGACCTGCCCACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCg                                                       <  2:26549/130‑1 (MQ=255)                                                                            tcgtcgATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGCAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGAt                                                     <  2:280008/133‑1 (MQ=255)                                                                             cgtcgATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGCAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGTAATATTGCCGCCAGGTTTGACGCCCATGCGATg                                                    <  1:123079/133‑1 (MQ=255)                                                                             cgtcgATATCCACCACCACCACCCCCGACGGTCGTATGCCGCCGTTAATGGCGCAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATg                                                    <  2:137906/133‑1 (MQ=255)                                                                             cctcgATATGCACCATCACCACCCTCGATGGTTGTATGCCGGCGTTAATGGCGCAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATg                                                    <  2:250708/131‑1 (MQ=255)                                                                                              cccaccCGCGGCGGTTGTATGCCGGCGTCAATGGCGCAATCCATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCg                                   <  2:187437/132‑1 (MQ=255)                                                                                              accaccCGCTACGGTTGTATGCCGCCGTTAATGGCGCAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCg                                   <  2:316619/133‑1 (MQ=255)                                                                                              accaccCGCGTCGGTCGTATGCCGCCGTCAATCGCGCAATCCATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCg                                   <  2:148443/133‑1 (MQ=255)                                                                                              accaccCGCGTCGGTAGTATGCCGTCGTCAATTGCGCAATCTATGGCGCCAATGCCCATCGGCCCTGCCCACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCg                                   <  2:145086/133‑1 (MQ=255)                                                                                              accaccCGCGACGGTTGTATGCCGCCGTTAATGGCGCAATCGATAGCGCGAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCg                                   <  1:414269/133‑1 (MQ=255)                                                                                              accaccCGCGACGGTTGTATGCCGCCGTTAATGGCGCAATCGATAGCGCCAATGCCTATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCg                                   <  2:268471/133‑1 (MQ=255)                                                                                              accaccCGCGACGGTTGTATGCCGCCGTTAATGGCGCAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCg                                   <  1:385685/133‑1 (MQ=255)                                                                                                caccCGCGACGGTTGTATGCCGCCGTTAATGGCGCAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCgtg                                 <  1:502589/133‑1 (MQ=255)                                                                                                      cgACGGTTGTATGCCGCCGTTAATGGCGCAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGa                           >  1:81748/1‑133 (MQ=255)                                                                                                          ggTTGTATGCCGCCGTTAATGGCGCAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAAt                       <  2:319013/133‑1 (MQ=255)                                                                                                                  gccgccGTTAATGGCGCAATCGATAGCGCCAATGCCCATCGGACCTGCACACGACAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGa               <  2:300390/133‑1 (MQ=255)                                                                                                                   ccgccgTTAATGGCGCAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGAt              >  2:80342/1‑133 (MQ=255)                                                                                                                      ccgTTAATGGCGCAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAg           <  2:58777/133‑1 (MQ=255)                                                                                                                       cgTTAATGGCGCAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGc          <  2:81748/133‑1 (MQ=255)                                                                                                                               gcgcAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCaataa  <  2:312356/133‑1 (MQ=255)                                                                                                                                  |                                                                                                                                  ACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAA  >  NC_000913/3076747‑3077005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑