Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,108,115	T→C	K347K (AAA→AAG)	speC ←	ornithine decarboxylase, biosynthetic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,108,115	0	T	C	100.0%	79.0 / NA	24	K347K (AAA→AAG)	speC	ornithine decarboxylase, biosynthetic
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/10);  total (14/10)

TGGTAGAAGCATGGAGCATAAAGGCGTTATTCAACCGCTTATGCGGGCAAAAACGCGCCTGTCCGCGGATATGGTTATCTTTTTTATGGATCTGCGACGTCTGTGAGAATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAA  >  NC_000913/3107994‑3108239                                                                                                                          |                                                                                                                             tGGTAGAAGCATGGAGCATAAAGGCGTTATTCAACCGCTTATGCGGGCAAAAACGCGCCTGTCCGCGGATATGGTTATCTTTTTTATGGATCTGCGACGTCTGTGAGAATCCCGCCTGCTGCTTGTGCACCGa                                                                                                                   <  2:368578/133‑1 (MQ=255)        aGCATGGAGCATAAAGGCGTTATTCAACCGCTTATGCGGGCAAAAACGCGCCTGTCCGCGGATATGGTTATCTTTTTTATGGATCTGCGACGTCTGTGAGAATCCCGCCTGCTGCTTGTGCACCGACTGAGTc                                                                                                            >  2:408968/1‑133 (MQ=255)              gAGCATAAAGGCGTTATTCAACCGCTTATGCGGGCAAAAACGCGCCTGTCCGCGGATATGGTTATCTTTTTTATGGATCTGCGACGTCTGTGAGAATCCCGCGTGCTGCTTGTGTACTGACTGAGTCACAAAg                                                                                                      >  1:239339/1‑133 (MQ=255)                       ggCGTTATTCAACCGCTTATGCGGGCAAAAACGCGCCTGTCCGCGGATATGGTTATCTTTTTTATGGATCTGCGACGTCTGTGAGAATCCCGCCTGCTGCTTGTGCACCGACTGAGTCACAAAGATCCCCGGa                                                                                             <  1:408968/133‑1 (MQ=255)                         cGTTATTCAACCGCTTATGCGGGCAAAAACGCGCCTGTCCGCGGATATGGTTATCTTTTTTATGGATCTGCGACGTCTGTGAGAATCCCGCCTGCTGCTTGTGCACCGACTGAGTCACAAAGATCCCCGGATc                                                                                           >  1:459458/1‑133 (MQ=255)                                cAACCGCTTATGCGGGCAAAAACGCGCCTGTCCGCGGATATGGTTATCTTTTTTATGGATCTGCGACGTCTGTGAGAATCCCGCCTGCTGCTTGTGCGCCGACTGAGTCACAAAGATCCCCGGATCGTTTTCg                                                                                    >  2:446820/1‑133 (MQ=255)                                  aCCGCTTATGCGGGCAAAAACGCGCCTGTCCGCGGATATGGTTATCTTTTTTATGGATCTGCGACGTCTGTGAGAATCCCGCCTGCTGCTTGTGCACCGACTGAGTCACAGAGATCCCCGGATCCTTTTCGtt                                                                                  >  1:188678/1‑133 (MQ=255)                                   ccGCTTATGCGGGCAAAAACGCGCCTGTCCGCGGATATGGTTATCTTTTTTATGGATCTGCGACGTCTGTGAGAATCCCGCCTGCTGCTTGTGCACCGACTGAGTCACAACGATCCCCGGATCGATTTCGTTa                                                                                 >  1:229886/1‑133 (MQ=255)                                    cGCTTATGCGGGCAAAAACGCGCCTGTCCGCGGATATGGTTATCTTTTTTATGGATCTGCGACGTCTGTGAGAATCCCGCCTGCTGCTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTaa                                                                                <  1:443521/133‑1 (MQ=255)                                           gCGGGCAAAAACGCGCCTGTCCGCGGATATGGTTATCTTTTTTATGGATCTGCGACGTCTGTGAGAATCCCGCCTGCTGCTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTaa                                                                         <  1:381898/133‑1 (MQ=255)                                                     aCGCGCCTGTCCGCGGATATGGTTATCTTTTTTATGGATCTGCGACGTCTGTGAGAATCCCGCCTGCTGCTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAgcggc                                                               <  2:229886/133‑1 (MQ=255)                                                         gcCTGTCCCCGGATATGGATATCTTTTTTAGGGATCTGCGACGTCTGTGAGAATCCCGCCTGCTGCTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGc                                                           <  1:204467/133‑1 (MQ=255)                                                                               cTTTTTTATGGATCTGCGACGTCTGTGAGAATCCCGCCTGCTGCTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATaaa                                     <  2:459458/133‑1 (MQ=255)                                                                                            cTGCGACGTCTGTGAGAATCCCGCCTGCTGCTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCg                        >  1:414058/1‑133 (MQ=255)                                                                                                   gTCTGTGAGACTCCCGCCTGCTGCTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCAcg                 <  2:223178/133‑1 (MQ=255)                                                                                                      tgtgAGAATCCCGCCTGCTGCTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCgg              >  1:3463/1‑133 (MQ=255)                                                                                                      tgtgAGAATCCCGCCTGCTGCTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCgg              >  1:346193/1‑133 (MQ=255)                                                                                                      tgtgAGAATCCCGCCTGCTGCTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCgg              >  2:440231/1‑133 (MQ=255)                                                                                                             aTCCCGCCTGCTGCTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCaaa       <  2:3463/133‑1 (MQ=255)                                                                                                             aTCCCGCCTGCTGCTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCaaa       <  1:105606/133‑1 (MQ=255)                                                                                                                ccGCCTGCTGCTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAg    >  1:90342/1‑133 (MQ=255)                                                                                                                ccGCCTGCTGCTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAg    >  1:124853/1‑133 (MQ=255)                                                                                                                  gCCTGCTGCTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCTACCCACGCGGAATCAAACAGaa  >  2:31802/1‑133 (MQ=255)                                                                                                                  gCCTGCTGCTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGaa  >  1:432463/1‑133 (MQ=255)                                                                                                                          |                                                                                                                             TGGTAGAAGCATGGAGCATAAAGGCGTTATTCAACCGCTTATGCGGGCAAAAACGCGCCTGTCCGCGGATATGGTTATCTTTTTTATGGATCTGCGACGTCTGTGAGAATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAA  >  NC_000913/3107994‑3108239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑