Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,226,934 T→C I234T (ATC→ACC)  ygjI → putative transporter YgjI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,226,9340TC96.2% 84.7 / NA 26I234T (ATC→ACC) ygjIputative transporter YgjI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (10/15);  minor base G (0/1);  total (10/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCATCCTCGCCGGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTT  >  NC_000913/3226815‑3227063
                                                                                                                       |                                                                                                                                 
cAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTAATGACCCTCACCGGGAtt                                                                                                                      >  1:157635/1‑133 (MQ=255)
     gggCTTTCCTCTGCGTCGCCACTTGGATCTTTATGGCCGCAGGTGTGGCGGAGTCCGTCGCTGTGTACGTTATCGCCGTCTAAGGTGGTTCGAAATCGTTCGTTAAAGCGATAACGCGCGCCGGGATTTTTAt                                                                                                                 <  2:410772/133‑1 (MQ=255)
     gggCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGATTTTTAt                                                                                                                 <  2:25412/133‑1 (MQ=255)
       gCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGATTTTTATCg                                                                                                               <  2:295657/133‑1 (MQ=255)
           tCCTCGGCGTTACCGCCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGATTTTTATCGGCGt                                                                                                           <  2:408696/133‑1 (MQ=255)
            ccTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGATTTTTATCGGCGTa                                                                                                          >  2:376207/1‑133 (MQ=255)
             cTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGATTTTTATCGGCGTAc                                                                                                         <  1:427009/133‑1 (MQ=255)
                       ccaccTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGATTTTTATCGGCGTACTGTATTCCGt                                                                                               <  1:433697/133‑1 (MQ=255)
                            tGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGATTTTTATCGGCGTACTGTATTCCGTctcct                                                                                          <  2:91033/133‑1 (MQ=255)
                            tGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGATTTTTATCGGCGTACTGTATTCCGTctcct                                                                                          <  1:5543/133‑1 (MQ=255)
                              gATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCg                                                                                        <  1:449678/133‑1 (MQ=255)
                                    tATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTg                                                                                  >  2:501367/1‑133 (MQ=255)
                                        gCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGAATTTTATCGGCGTGCTGGATTCCGTCGCCTCGGTGCTGATTa                                                                              >  2:206696/1‑133 (MQ=255)
                                           gCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACg                                                                           >  1:78573/1‑133 (MQ=255)
                                                       gAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCC‑‑‑CCGGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAgcagca                                                            <  1:396621/133‑1 (MQ=255)
                                                            cGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGAGTAACGTATTCGTCCGCAGCaaa                                                          >  1:194165/1‑133 (MQ=255)
                                                                    tgtACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGaa                                                  >  1:409132/1‑133 (MQ=255)
                                                                              cgacgTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACcggc                                        >  1:440147/1‑133 (MQ=255)
                                                                              cgacgCCAAAGGCGGTTCGAAATCGATCGGTAAAGGGAACACCCTCGCCGGGATTTTTTTCGGCGTACTGTATTCCGGCTCCTCGGCGCTGATGAACGTCTTCGTCACCAGCAAAGAGTTGACATTTACcggt                                        >  2:38895/1‑132 (MQ=255)
                                                                                acgTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACcggcgg                                      >  1:384721/1‑133 (MQ=255)
                                                                                  gTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGAt                                    <  2:384721/133‑1 (MQ=255)
                                                                                  gTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGAt                                    <  2:440147/133‑1 (MQ=255)
                                                                                      aaGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGt                                <  1:376207/133‑1 (MQ=255)
                                                                                      aaGGCGGTTCAAAATGGTTCGTTAAAGTGATCCCCCTCGCCCGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGt                                <  2:194165/133‑1 (MQ=255)
                                                                                       aGGCGGTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTg                               <  1:38895/133‑1 (MQ=255)
                                                                                           ggTTCGAAATCGTTCGTTAAAGTGATCACCCTCGCCGGGTTTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGCCAGCAGCAAAGAATTGCAATTTACCAGCGGTCCGGTGCAgg                           >  2:217357/1‑133 (MQ=255)
                                                                                                       ttcgttAAAGTGATCACCCTCGCCGGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCa               <  2:417952/133‑1 (MQ=255)
                                                                                                            tAAAGTGAGCACCCTGGCAGGGCTTTTTATGGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATggcg          <  1:217357/133‑1 (MQ=255)
                                                                                                              atgTGATCAGTCTCGACGGGATTTCTCTCGGCCTACTGTATCCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATggcggc        <  2:249552/131‑1 (MQ=255)
                                                                                                                    tcaccCTCGCCGGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCAGTGGAGGAATTCCACGACATGGCGGCGTAttt  >  2:182979/1‑133 (MQ=255)
                                                                                                                       |                                                                                                                                 
CAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAGTGATCATCCTCGCCGGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTT  >  NC_000913/3226815‑3227063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑