Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,236,318 T→C V120A (GTG→GCG)  ygjP → putative metal‑dependent hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,236,3180TC97.3% 119.8 / NA 37V120A (GTG→GCG) ygjPputative metal‑dependent hydrolase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (14/22);  total (15/22)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAAAAACGCGCTCGGGTTACATACCGCTGTTTCTCGTGTGCAGGGCGGCAAGCTGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGTGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCT  >  NC_000913/3236189‑3236444
                                                                                                                                 |                                                                                                                              
tAAAAAACGCGCTCGGGTTACATACCGCTGTTTCTCGTGTGCAGGGCGGCAAGCTGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCa                                                                                                                             <  1:405001/133‑1 (MQ=255)
tAAAAAACGCGCTCGGGTTACATACCGCTGTTTCTCGTGTGCAGGGCGGCAAGCTGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCa                                                                                                                             >  2:325673/1‑133 (MQ=255)
  aaaaaCGCGCTCGGGTTACATACCGCTGTTTCTCGTGTGCAGGGCGGCAAGCTGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCGGGAGCCGGTTTTGCGCATGATAGTGGCGCACg                                                                                                                           >  2:133275/1‑133 (MQ=255)
                       aCCGCTGTTTGTCGTTTGCAGGGCTGCATGCTCAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGgaga                                                                                                      <  2:151363/133‑1 (MQ=255)
                       aCCGCTGTTTCTCGTGTGCAGGGCGGCAAGCTGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGgaga                                                                                                      <  2:320021/133‑1 (MQ=255)
                       aCCGCTGTATCTCGTATGAAGGGCGGCAAGTTGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGgaga                                                                                                      <  1:250577/133‑1 (MQ=255)
                         cGCTGTTTCTCGTGTGCAGGGCGGCAAGCTGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGaaa                                                                                                    >  1:335447/1‑133 (MQ=255)
                         cGCTGTTTCTCGTGTGCAGGGCGGCAAGCTGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCATGGTGCACGAGCTGGCACACCGGAAGGAGaaa                                                                                                    >  2:175150/1‑133 (MQ=255)
                          gCTGTTTCTCGTGTGCAGGGCGGCAAGCTGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAag                                                                                                   >  1:371551/1‑133 (MQ=255)
                              tttCTCGTGTGCAGGGCGGCAAGCTGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCa                                                                                               >  1:367044/1‑133 (MQ=255)
                                   cGTGTGCAGGGCGACAAGCTGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACa                                                                                          >  2:514290/1‑133 (MQ=255)
                                    gtgtGCAGGGCGGCAAGCTGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACaa                                                                                         <  2:421717/133‑1 (MQ=255)
                                      gtgCAGGGCGGCAAGCTGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAg                                                                                       >  2:49716/1‑133 (MQ=255)
                                            ggcggcAAACTGAAATCATAGGCGGAGATCAGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGtttt                                                                                 <  1:133275/133‑1 (MQ=255)
                                               ggcAAGCTGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGGAGGAGAATGAGCATTACAAAGCGTTTTAcc                                                                              >  1:208885/1‑133 (MQ=255)
                                               ggcAAGCTGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTAcc                                                                              <  2:335447/133‑1 (MQ=255)
                                                 cAAGCTGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAg                                                                            <  1:49716/133‑1 (MQ=255)
                                                  aaGCTGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAgt                                                                           <  2:60041/133‑1 (MQ=255)
                                                      tGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAgttgtg                                                                       <  1:24777/133‑1 (MQ=255)
                                                      tGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAgttgtg                                                                       <  2:495564/133‑1 (MQ=255)
                                                      tGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCACCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGATAAAAAGCTTAACAAAGAGTTTGACCAgttgtg                                                                       >  2:177157/1‑133 (MQ=255)
                                                                     agaTCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAAcc                                                        <  2:367044/133‑1 (MQ=255)
                                                                      gaTCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAAccc                                                       >  1:428630/1‑133 (MQ=255)
                                                                       aTCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCa                                                                 <  1:467290/122‑1 (MQ=255)
                                                                       aTCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCa                                                                 >  2:467290/1‑122 (MQ=255)
                                                                                         tttCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGt                                    >  1:483333/1‑133 (MQ=255)
                                                                                          ttCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGtt                                   <  2:428630/133‑1 (MQ=255)
                                                                                          ttCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGtt                                   <  2:429602/133‑1 (MQ=255)
                                                                                           tCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTc                                  >  1:48821/1‑133 (MQ=255)
                                                                                            cGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCg                                 >  1:35670/1‑133 (MQ=255)
                                                                                             gCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGa                                <  1:514290/133‑1 (MQ=255)
                                                                                                aCGCGCCGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGacac                             <  2:35670/133‑1 (MQ=255)
                                                                                                      cGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGTGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACt                       <  1:500096/133‑1 (MQ=255)
                                                                                                      cGGAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACCCCCGACt                       <  2:371551/133‑1 (MQ=255)
                                                                                                        gAACCGTTTTTGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATACGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACtgt                     <  1:325673/133‑1 (MQ=255)
                                                                                                                tttGCGCATGATCGTGGCGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACg             <  2:483333/133‑1 (MQ=255)
                                                                                                                           tCGTGGCGCACGAGCCGGCGCACCGGAAGGAGTAAGAGCATTACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCt  <  2:208885/133‑1 (MQ=255)
                                                                                                                                 |                                                                                                                              
TAAAAAACGCGCTCGGGTTACATACCGCTGTTTCTCGTGTGCAGGGCGGCAAGCTGAAAGCAAAGGTGGAGATCCGCGTCGCTACCGTGTTTCGCAACGCGCCGGAACCGTTTTTGCGCATGATCGTGGTGCACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCT  >  NC_000913/3236189‑3236444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑