Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,278,656 T→C T4A (ACC→GCC)  agaR ← DNA‑binding transcriptional repressor AgaR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,278,6560TC100.0% 128.8 / NA 38T4A (ACC→GCC) agaRDNA‑binding transcriptional repressor AgaR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/19);  total (19/19)

GTCACAGTAGATACGCCATACAATGCCGACAGATCGTTAACCTGCACACTCCCTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGTATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTAACGCCTTTCTATTAAGCAAATGCAAGCCCACCTTGCCCATTGACGCAAGCTACTCTCGTTTCAGTGA  >  NC_000913/3278526‑3278779
                                                                                                                                  |                                                                                                                           
gTCACAGTAGATACGCCATACAATGCCGACAGATCGTTAACCTGCACACTCCCTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCAt                                                                                                                           <  2:281260/133‑1 (MQ=255)
    caGTAGATACGCCATACAATGCCGACAGATCGTTAACCTGCACACTCCCTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACt                                                                                                                       <  1:310116/133‑1 (MQ=255)
          aTACGCCATACAATGCCGACAGATCGTTAACCTGCACACTCCCTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACt                                                                                                                       <  2:296159/127‑1 (MQ=255)
          aTACGCCATACAATGCCGACAGATCGTTAACCTGCACACTCCCTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACt                                                                                                                       >  1:296159/1‑127 (MQ=255)
             cGCCATACAATGCCGACAGATCGTTAACCTGCACACTCCCTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAg                                                                                                              <  2:440351/133‑1 (MQ=255)
               ccATACAATGCCGACAGATCGTTAACCTGCACACTCCCTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTc                                                                                                            >  2:365084/1‑133 (MQ=255)
               ccATACAATGCCGACAGATCGTTAACCTGCACACTCCCTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTc                                                                                                            >  1:486204/1‑133 (MQ=255)
               ccATACAATGCCGACAGATCGTTAACCTGCACACTCCCTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTAACCAGCACCGTCGGCATTACTCATAGTAAgcc                                                                                                            >  2:242662/1‑131 (MQ=255)
                 aTACAATGCCGACAGATCGTTAACCTGCACACTCCCTTGCTGTCGCAGACGCTGAATGATTTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCt                                                                                                          >  1:58322/1‑133 (MQ=255)
                 aTACAATGCCGACAGATCGTTAACCTGCACACTCCCTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGGAGCATCGGCATTGCTCAGAGTAAGTCCt                                                                                                          >  2:186930/1‑133 (MQ=255)
                   aCAATGCCGACAGATCGTTAACCTGCACACTCCCTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCttt                                                                                                        >  1:351146/1‑133 (MQ=255)
                      aTGCCGACAGATCGTTAACCTGCACACTCCCTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGAGTCGGCATTACGCATAGTAAGTCCTTTCGt                                                                                                     >  1:174483/1‑133 (MQ=255)
                             cAGATCGTTAACCTGCACACTCCCTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACtt                                                                                              <  2:486204/133‑1 (MQ=255)
                                          tGCACACTCCCTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCg                                                                                 >  2:98094/1‑133 (MQ=255)
                                            cacaCTCCCTTGCTGTCGCAGACGCTGAATGATTTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGtt                                                                               <  2:58322/133‑1 (MQ=255)
                                            cacaCTCCCTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGtt                                                                               >  1:509255/1‑133 (MQ=255)
                                            cacaCTCCCTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTAATCATAGTAGGTCATTTAGTAAAACTATCGTATCATTTCGtt                                                                               >  2:156045/1‑133 (MQ=255)
                                             acacTCCCTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAATCCGTTCATAAAACTTTCGTTAGATTTCGttt                                                                              >  2:225718/1‑133 (MQ=255)
                                                 tCCCTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGcc                                                                          >  2:281351/1‑133 (MQ=255)
                                                   ccTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCACTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTa                                                                        >  1:498663/1‑133 (MQ=255)
                                                      tGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTa                                                                     >  1:281200/1‑133 (MQ=255)
                                                       gCTGTCGCCGACGCTCAATGATCTGTTCTCTTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTaa                                                                    <  1:156045/133‑1 (MQ=255)
                                                       gCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTaa                                                                    <  1:365084/133‑1 (MQ=255)
                                                         tGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTAACg                                                                  <  2:367242/133‑1 (MQ=255)
                                                            cGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTAACGCCt                                                               <  2:351146/133‑1 (MQ=255)
                                                            cGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTAACGCCt                                                               <  1:260053/133‑1 (MQ=255)
                                                            cGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTAACGCCt                                                               <  2:448821/133‑1 (MQ=255)
                                                                  cGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTAACGCCTTTCTAt                                                         <  1:281351/133‑1 (MQ=255)
                                                                   gCTGAATGGTCTGTTCTCCTCGCTCGCTGGAGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTAACGCCTTTCTAtt                                                        <  1:164855/133‑1 (MQ=255)
                                                                           aTCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTAACGCCTTTCTATTAAGCAAAt                                                >  2:53287/1‑133 (MQ=255)
                                                                                ttCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTAACGCCTTTCTATTAAGCAAATGCAAg                                           <  1:387915/133‑1 (MQ=255)
                                                                                ttCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTAACGCCTTTCTATTAAGCAAATGCAAg                                           <  2:509255/133‑1 (MQ=255)
                                                                                     gtcgCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTAACGCCTTTCTATTAAGCAAATGCAAGCCCAc                                      >  1:430388/1‑133 (MQ=255)
                                                                                        gctcgctGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTAATGCCGTTCGATTAAGCAAATGCCAGCCCACCtt                                   >  2:214805/1‑133 (MQ=255)
                                                                                                     aGTCACTCGCTTCTCGCCAGAACCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTAACGCCTTTCTATTAAGCAAATGCAAGCCCACCTTGCCCATTGACGCa                      <  1:242662/133‑1 (MQ=255)
                                                                                                               ttCTCACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTAACGCCTTTCTATTAAGCAAATGCAAGCCCACCTTGCCCATTGACGCAAGCTACtctc            <  1:471397/133‑1 (MQ=255)
                                                                                                                 ctcACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTAACGCCTTTCTATTAAGCAAATGCAAGCCCACCTTGCCCATTGACGCAAGCTACTCTCGt          >  2:222267/1‑133 (MQ=255)
                                                                                                                   cACCTGAAGCGTCGGCATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTAACGCCTTTCTATTAAGCAAATGCAAGCCCACCTTGCCCATTGACGCAAGCTACTCTCGttt        <  2:498663/133‑1 (MQ=255)
                                                                                                                         aaGCGCCGGCATTACTCATAGTACGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTAACGCCTTTCTATTAAGCAAATGCAAGCCCACCTTGCCCATTGACGCAAGCTACTCTCGTTTCAGTGa  >  2:473946/1‑133 (MQ=255)
                                                                                                                                  |                                                                                                                           
GTCACAGTAGATACGCCATACAATGCCGACAGATCGTTAACCTGCACACTCCCTTGCTGTCGCAGACGCTGAATGATCTGTTCTCGTCGCTCGCTGGTGCCAGTCACTCGCTTCTCACCTGAAGCGTCGGTATTACTCATAGTAAGTCCTTTCGTAAAACTTTCGTTTCATTTCGTTTTGCCTATTAACGCCTTTCTATTAAGCAAATGCAAGCCCACCTTGCCCATTGACGCAAGCTACTCTCGTTTCAGTGA  >  NC_000913/3278526‑3278779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑