Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 262,313 C→T P271S (CCC→TCC)  proA → glutamate‑5‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913262,3130CT98.1% 166.8 / NA 52P271S (CCC→TCC) proAglutamate‑5‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (24/27);  minor base G (1/0);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTT  >  NC_000913/262187‑262441
                                                                                                                              |                                                                                                                                
gTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCAt                                                                                                                            <  1:96976/133‑1 (MQ=255)
gTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCAt                                                                                                                            >  1:265016/1‑133 (MQ=255)
   gatgaAAGGGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTaa                                                                                                                         >  2:425292/1‑133 (MQ=255)
    atgaAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAg                                                                                                                        >  2:335022/1‑133 (MQ=255)
     tgaAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGc                                                                                                                       >  1:381630/1‑133 (MQ=255)
              agagATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATg                                                                                                              >  2:309090/1‑133 (MQ=255)
              agagATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGAGAGCTTCCTGTCTGCGTTAAGCGAACAAATg                                                                                                              >  1:238813/1‑133 (MQ=255)
                  aTCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAACTTACTGGCCGCATTAAGCAAACAAATGGCgg                                                                                                          >  2:196018/1‑133 (MQ=255)
                     gCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGcaaacaaa                                                                                                                >  2:405324/1‑124 (MQ=255)
                     gCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGcaaacaaa                                                                                                                <  1:405324/124‑1 (MQ=255)
                     gCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGaaa                                                                                                       >  2:98586/1‑133 (MQ=255)
                     gCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGaaa                                                                                                       >  1:366766/1‑133 (MQ=255)
                           gCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAgcggcg                                                                                                 <  1:276899/133‑1 (MQ=255)
                             aTTAAAAGTGATCGTCAACGCGAATACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTg                                                                                               <  2:169907/133‑1 (MQ=255)
                              ttAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGa                                                                                              >  1:362899/1‑133 (MQ=255)
                              ttAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGa                                                                                              >  1:367421/1‑133 (MQ=255)
                              ttAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGa                                                                                              >  1:348338/1‑133 (MQ=255)
                                  aaGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACAtt                                                                                          <  2:392887/133‑1 (MQ=255)
                                          gTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGACAGCAGCTTGACATCACACGCAg                                                                                  >  1:216959/1‑133 (MQ=255)
                                           tCAACGCGAATACTCGGCGTCCGAGCTCATGTAATACGGTTCAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGa                                                                                 <  2:235333/133‑1 (MQ=255)
                                           tCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGTTAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGa                                                                                 <  2:375211/133‑1 (MQ=255)
                                               cgcgAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCa                                                                             >  2:429938/1‑133 (MQ=255)
                                                gcgAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAg                                                                            <  2:366766/133‑1 (MQ=255)
                                                           gCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGgcg                                                                 <  1:335022/133‑1 (MQ=255)
                                                           gCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGgcg                                                                 <  1:309090/133‑1 (MQ=255)
                                                           gCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGgcg                                                                 <  2:362899/133‑1 (MQ=255)
                                                           gCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGgcg                                                                 <  1:98586/133‑1 (MQ=255)
                                                                 gAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTg                                                           <  1:126518/133‑1 (MQ=255)
                                                                         gTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGcaggcagg                                                   <  2:265016/133‑1 (MQ=255)
                                                                         gTAATACGGACGAAACGTTGCTGGTGAACAAATACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGcaggcagg                                                   <  2:238813/133‑1 (MQ=255)
                                                                             tACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACGGGCACAATTGCAGTCAGGCCCt                                               >  1:219673/1‑133 (MQ=255)
                                                                                 gTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGa                                           >  2:441438/1‑133 (MQ=255)
                                                                                 gTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGa                                           >  2:93137/1‑133 (MQ=255)
                                                                                       aCGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAggtgg                                     <  1:425292/133‑1 (MQ=255)
                                                                                         gTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGtt                                   <  2:67308/133‑1 (MQ=255)
                                                                                          ttGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTg                                  <  2:344398/133‑1 (MQ=255)
                                                                                           tGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCGGCACTGGCTCAGGTGCGGGCAGGCCGTGCGGAGGTGGTTGc                                 >  2:146841/1‑133 (MQ=255)
                                                                                                gTGAATAAAAACATCCCCGATAGCTTCCCGTCCGCATTAAGGAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTa                            <  2:195975/133‑1 (MQ=255)
                                                                                                    aTAAAAACATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGc                        >  2:58075/1‑133 (MQ=255)
                                                                                                     tAAAAACATCGCCGGTAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGcc                       >  2:329810/1‑133 (MQ=255)
                                                                                                      aaaaaCATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCg                      <  1:441438/133‑1 (MQ=255)
                                                                                                      aaaaaCATAGCCGCTTGCGTCCGGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCg                      <  1:218902/133‑1 (MQ=255)
                                                                                                        aaaCATCGCCGATAGGTTACTGTCCGCATTCAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGaa                    <  1:146841/133‑1 (MQ=255)
                                                                                                        aaaCATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGaa                    <  1:93137/133‑1 (MQ=255)
                                                                                                        aaaCATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGaa                    <  2:443022/133‑1 (MQ=255)
                                                                                                        aaaCATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGaa                    >  1:443022/1‑133 (MQ=255)
                                                                                                          aCATCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAaga                  <  1:331240/133‑1 (MQ=255)
                                                                                                            aTCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGt                <  2:23844/133‑1 (MQ=255)
                                                                                                             tCGCCGATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGCTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTa               >  1:329226/1‑133 (MQ=255)
                                                                                                                  gATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACg          >  2:356828/1‑133 (MQ=255)
                                                                                                                  gATAGCTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACg          <  2:348338/133‑1 (MQ=255)
                                                                                                                       cTTCCTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGCGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAg     >  2:411045/1‑133 (MQ=255)
                                                                                                                          ccTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGttt  <  1:58075/133‑1 (MQ=255)
                                                                                                                          ccTGTCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTACTGTTAAAGCCGAAGAGTATGTCGATGAGttt  >  2:178960/1‑133 (MQ=255)
                                                                                                                              |                                                                                                                                
GTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTT  >  NC_000913/262187‑262441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑