Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,304,937	A→G	V294A (GTA→GCA)	mtr ←	tryptophan:H(+) symporter Mtr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,304,937	0	A	G	100.0%	89.1 / NA	27	V294A (GTA→GCA)	mtr	tryptophan:H(+) symporter Mtr
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/16);  total (11/16)

CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTAACGCCTGTACCAGCACATCAATATTACCGCCCTTCTCTGCAATACCGATAAACTCCGGACGC  >  NC_000913/3304808‑3305062                                                                                                                                  |                                                                                                                              ccccccACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTGCCg                                                                                                                            <  2:472412/133‑1 (MQ=255)       acaACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGt                                                                                                                      >  2:481498/1‑133 (MQ=255)        caACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGTCAAAGAACGAACGCGCGACCGCAAAGtt                                                                                                                     >  2:230489/1‑133 (MQ=255)         aaCTGGCGGGGCAAATGTCAGCAATGCCTTTTCCAAGCGGCTCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGttt                                                                                                                    <  2:156462/133‑1 (MQ=255)             ggCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGTTTgaga                                                                                                                >  1:447292/1‑133 (MQ=255)                          tCAGCAATGCCGTTTTCAGGCGGCCCACAGGCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGTTTGAGAACACGACcagcag                                                                                                   <  1:222615/133‑1 (MQ=255)                           cAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGTTTGAGAACACGACcagcagc                                                                                                  <  1:481498/133‑1 (MQ=255)                                                    acaGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTa                                                                         <  1:491803/133‑1 (MQ=255)                                                    acaGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTa                                                                         <  1:508627/133‑1 (MQ=255)                                                             tcgtcgAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTa                                                                <  2:456329/133‑1 (MQ=255)                                                                       cAAACAGATCTGCCACATAGTCCAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTAACGCCTGTAc                                                      <  1:143498/133‑1 (MQ=255)                                                                         aaCAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGGACGGCGGTTAACGGCTGTACCa                                                    >  1:134001/1‑133 (MQ=255)                                                                          aCAGATCTTCCAGATTGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTAACGCCTGTACCAg                                                   <  1:152918/133‑1 (MQ=255)                                                                              aTCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTAACGCCTGTACCAg                                                   >  1:374310/1‑129 (MQ=255)                                                                              aTCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTAACGCCTGTACCAg                                                   <  2:374310/129‑1 (MQ=255)                                                                                cTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTAACGCCTGTACCAGCACATc                                             >  1:50626/1‑133 (MQ=255)                                                                                          gTCAAACAAACCCAGTGTTACGCCGAGGAACGGACTCGCTGACGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTAACGCCTGTACCAGCACATCAATATTACCg                                   <  2:201023/133‑1 (MQ=255)                                                                                          gTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTAACGCCTGTACCAGCACATCAATATTACCg                                   <  2:50626/133‑1 (MQ=255)                                                                                           tCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTAACGCCTGTACCAGCACATCAATATTACCg                                   <  1:504596/132‑1 (MQ=255)                                                                                           tCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTAACGCCTGTACCAGCACATCAATATTACCg                                   >  2:504596/1‑132 (MQ=255)                                                                                           tCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTAACGCCTGTACCAGCACATCAATATTACCGc                                  >  1:47520/1‑133 (MQ=255)                                                                                             aaacaaacCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTAACGCCTGTACCAGCACATCAATATTACCGccc                                >  1:86459/1‑133 (MQ=255)                                                                                               acaaacCCAGCGTTACGCCGAGGATCGATCTCTCTGCCGCACAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTAACGCCTGTACCAGCACATCAATATTACCGCCCtt                              <  2:134001/133‑1 (MQ=255)                                                                                               acaaacCCAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTAACGCCTGTACCAGCACATCAATATTACCGCCCtt                              <  1:407594/133‑1 (MQ=255)                                                                                                     ccAGCGTTACGCCGAGGAACGAACTCGCTGCCGCAAAGTTTGAGAAAACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTAACGCCTGTACCAGCACATCAATATTACCGCCCTTCTCTGc                        >  2:317902/1‑133 (MQ=255)                                                                                                             aCGCCGAGGAACGAACTCGCTGCCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTAACGCCTGTACCAGCACATCAATATTACCGCCCTTCTCTGCAATACCGa                >  2:354107/1‑133 (MQ=255)                                                                                                                         gaacTCGCTGCCGCAAAGTTTGAGTACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTAACGCCTGTACCAGCACATCAATATTACCGCCCTTCTCTGCAATACCGATAAACTCCGGAc    >  2:346302/1‑133 (MQ=255)                                                                                                                           acTCGCTGCCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTAACGCCTGTACCAGCACATCAATATTACCGCCCTTCTCTGCAATACCGATAAACTCCGGAcgc  <  2:86459/133‑1 (MQ=255)                                                                                                                                  |                                                                                                                              CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACCAGCAGCAGATCCAGACTACGGCTGTTCAGTACGCCGCTTAACGCCTGTACCAGCACATCAATATTACCGCCCTTCTCTGCAATACCGATAAACTCCGGACGC  >  NC_000913/3304808‑3305062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑