Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,414,734	T→C	F291L (TTT→CTT)	acrE →	multidrug efflux pump membrane fusion lipoprotein AcrE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,414,734	0	T	C	100.0%	96.8 / NA	29	F291L (TTT→CTT)	acrE	multidrug efflux pump membrane fusion lipoprotein AcrE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/14);  total (15/14)

GTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGC  >  NC_000913/3414612‑3414861                                                                                                                           |                                                                                                                                gTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGCTTgtgcgtgc                                                                                                                       <  2:452321/133‑1 (MQ=255) gTCAAACCCATCCCCCGACAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGCTTgtgcgtgc                                                                                                                       <  1:143548/133‑1 (MQ=255)                tGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAgg                                                                                                        >  2:498570/1‑133 (MQ=255)                         cGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAAcc                                                                                               >  2:108053/1‑133 (MQ=255)                            tGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGAATGCTTGTACGTACACGGATTGATGGAGGCCTCCAACCTGa                                                                                            >  2:199687/1‑133 (MQ=255)                              cAATTCTCGGATGTGACCTTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACg                                                                                          <  1:170186/133‑1 (MQ=255)                               aaTTCTCCTATGTGATCGTTGAGGAAAGCAACGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGc                                                                                         <  1:195194/133‑1 (MQ=255)                               aaTTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGc                                                                                         <  1:52443/133‑1 (MQ=255)                               aaTTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGc                                                                                         >  2:361112/1‑133 (MQ=255)                               aaTTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGc                                                                                         <  1:449658/133‑1 (MQ=255)                                    tcCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGATGGCATCTAACCTGACGCCATTc                                                                                    >  1:243876/1‑133 (MQ=255)                                                       aaaGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGt                                                                 <  1:108053/133‑1 (MQ=255)                                                              cGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGc                                                          >  2:392056/1‑133 (MQ=255)                                                                gCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGcac                                                        >  1:344937/1‑133 (MQ=255)                                                                                   tGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCGCGCAACAAGGCGATAGTCGCACACCACGTGCTGATGCAAcc                                     >  2:192757/1‑133 (MQ=255)                                                                                   tGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAAcc                                     <  1:331313/133‑1 (MQ=255)                                                                                   tGTCTTCCCTAACCCCCAGCATTCGCTTTTTCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAAcc                                     <  1:177046/133‑1 (MQ=255)                                                                                    gTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCGGCACACCGTGTGGGGATGCAACCg                                    >  2:177046/1‑133 (MQ=255)                                                                                       ttCCCTAACCCGCAACATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGc                                 >  2:309006/1‑133 (MQ=255)                                                                                          ccTAACCCGCAACATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGa                              <  1:373636/133‑1 (MQ=255)                                                                                               cccGCATCATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGtt                         >  2:464461/1‑133 (MQ=255)                                                                                               cccGCAACATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGtt                         >  1:378161/1‑133 (MQ=255)                                                                                                 cGCAACATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTaa                       >  1:29530/1‑133 (MQ=255)                                                                                                   cAACATACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACg                     >  2:125328/1‑133 (MQ=255)                                                                                                   cAACATACGCTTCTGCCGGGGATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACg                     >  2:424652/1‑133 (MQ=255)                                                                                                        tACGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATaaa                >  1:115695/1‑133 (MQ=255)                                                                                                          ctcTTTTGCGAGGTATGCTTGTGGTTGCACGGGTTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAg              <  2:243876/131‑1 (MQ=255)                                                                                                          cGCTTTTGGCGGGTATGCGTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAg              <  1:192757/133‑1 (MQ=255)                                                                                                          cGCTTTTGCCGGGTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAg              <  1:464461/133‑1 (MQ=255)                                                                                                                      gTATGCTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAgc  <  2:115695/133‑1 (MQ=255)                                                                                                                           |                                                                                                                                GTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGC  >  NC_000913/3414612‑3414861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑