Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,422,481	G→A	E16K (GAA→AAA)	yhdZ →	putative ABC transporter ATP‑binding subunit YhdZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,422,481	0	G	A	100.0%	112.7 / NA	33	E16K (GAA→AAA)	yhdZ	putative ABC transporter ATP‑binding subunit YhdZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/11);  total (22/11)

GGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGGAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTAACCGTGCAACCGGGAGAACGGATCGTTCTGTGTGGCCCTTCAGGTTCCGGTAAATCGACAACCATTCG  >  NC_000913/3422352‑3422602                                                                                                                                  |                                                                                                                          ggATCTTCTGTTTCATCATGTCGCTCTATAGCAAGTATCTGGAAAAACGTTTTAACACCGGGCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGaaaa                                                                                                                        <  2:144658/133‑1 (MQ=255)    tcttctGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACg                                                                                                                     >  2:331404/1‑133 (MQ=255)           tttCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATaa                                                                                                              <  1:487250/133‑1 (MQ=255)            ttCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATaaa                                                                                                             <  1:355857/133‑1 (MQ=255)              cAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATaa                                                                                                              >  2:258703/1‑130 (MQ=255)              cAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATaa                                                                                                              <  1:258703/130‑1 (MQ=255)                gCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGt                                                                                                         <  1:271270/133‑1 (MQ=255)                     tCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGa                                                                                                    >  1:390541/1‑133 (MQ=255)                                         ggAAAAACGTTTTAACACCGGGCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGaaaaa                                                                                >  2:483646/1‑133 (MQ=255)                                         ggAAAAACGTTTTAACACCGGGCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGaaaaa                                                                                >  2:417695/1‑133 (MQ=255)                                          gAAAAACGTTTTAACACCGGGCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAat                                                                               <  1:419240/133‑1 (MQ=255)                                              aaCGTTTTAACACCGGGCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGACAATAAATCGTAGGGAAAATTCCATGTTTTGAAAAATATaa                                                                           >  2:185194/1‑133 (MQ=255)                                                    ttAACACCGGGCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTaa                                                                     <  1:34443/133‑1 (MQ=255)                                                           cGGGCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATATTTCGAAAAATATAAATTTAACCGTGCa                                                              >  2:159610/1‑133 (MQ=255)                                                            gggCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTAACCGTGCaa                                                             >  1:25288/1‑133 (MQ=255)                                                             ggCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAGAATATAGATTTAAGCGTGCAAc                                                            >  1:132538/1‑133 (MQ=255)                                                             ggCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTAACCGTGCAAc                                                            >  1:33142/1‑133 (MQ=255)                                                              gCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTAACCGTGCAAcc                                                           >  1:485235/1‑133 (MQ=255)                                                               cGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTAACCGTGCAACCg                                                          <  1:276847/133‑1 (MQ=255)                                                                  acacCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTGACCGTGCAACCGGga                                                       >  1:214219/1‑133 (MQ=255)                                                                     ccGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTGACCGTGCAACCGGGAGaa                                                    >  1:167999/1‑133 (MQ=255)                                                                        cATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTAACGGTGCAACCGAGAGAACgg                                                 >  1:154846/1‑133 (MQ=255)                                                                        cATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTAACCGTGCAACCGGGAGAACgg                                                 <  1:109583/133‑1 (MQ=255)                                                                        cATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTAACCGTGCAACCGGGAGAACgg                                                 >  2:370069/1‑133 (MQ=255)                                                                         aTTGAGGACACTATGCGCCAAACTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTAACCGTGCAACCGGGAGAACGGa                                                <  1:235022/133‑1 (MQ=255)                                                                                    tATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTAACCGTGCAACTGGGCGATCGGATCATTCtgtgt                                     >  1:238953/1‑133 (MQ=255)                                                                                              aTTTTACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTAACCGTGCAACCGGGAGAACGGATCGTTCTGTGTGGCCCTTCAg                           >  1:261755/1‑133 (MQ=255)                                                                                               gtttACTGCAACCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTAACCGTGCAACCGGGAGAACGGATCGTTCTGTGTGGCCCTTCAgg                          <  1:429645/132‑1 (MQ=255)                                                                                                         aCCTGCTAACGCTATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTAACCGTGCAACCGGGAGAACGGATCGTTCTTTGTGGCCCATCAGTCTCCGGTaaa                >  2:224271/1‑133 (MQ=255)                                                                                                         aCCTGCTAACGCGATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTAACCGTGCAACCGGGAGAACGGATCGTTCTGTGTGGCCCTTCAGGTTCCGGTaaa                >  2:444973/1‑133 (MQ=255)                                                                                                                    cgATGATTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTAACCGTGCAACCGGGAGAACGGATCGTTCTGTGTGGCCCTTCAGGTTCCGGTAAATCGACAACCAt     >  1:462106/1‑133 (MQ=255)                                                                                                                      atgatTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTAACCGTGCAACCGGGAGAACGGATCGTTCTGTGTGGCCCTTCAGGTTCCGGTAAATCGACAACCATTc   >  2:409422/1‑133 (MQ=255)                                                                                                                      atgatTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTAACCGTGCAACCGGGAGAACGGATCGATCTGTGTGACCCGTCAAGTTCCGGTAAATCGACAACCATTc   >  2:177178/1‑133 (MQ=255)                                                                                                                       tgatTACGCTGAAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTAACCGTGCAACCGGGAGAACGGATCGTTCTGTGTGGCCCTTCAGGTTCCGGTAAATCGACAACCATTCg  <  1:331404/133‑1 (MQ=255)                                                                                                                                  |                                                                                                                          GGATCTTCTGTTTCAGCATGTCGCGCTATAGCCAGTATCTGGAAAAACGTTTTAACACCGGGCGTACACCGCATTGAGGACACTATGAGCCAAATTTTACTGCAACCTGCTAACGCGATGATTACGCTGGAAAACGTCAATAAATGGTATGGACAATTCCATGTTTTGAAAAATATAAATTTAACCGTGCAACCGGGAGAACGGATCGTTCTGTGTGGCCCTTCAGGTTCCGGTAAATCGACAACCATTCG  >  NC_000913/3422352‑3422602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑