Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,438,922	A→T	I70N (ATC→AAC)	zntR ←	DNA‑binding transcriptional activator ZntR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,438,922	0	A	T	100.0%	72.2 / NA	23	I70N (ATC→AAC)	zntR	DNA‑binding transcriptional activator ZntR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (9/14);  total (9/14)

GCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAATCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGACTCCAGACTGAAACCTAGTTGTCTGGCATGGCGGATAAATTTCAATCGCTGGAGATCGCTTTCGGTATATAGGCGAAACCCACCTTCAGTACGCACT  >  NC_000913/3438804‑3439041                                                                                                                       |                                                                                                                        gCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAATCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGTTCGACAGCAACTcg                                                                                                           <  1:448579/133‑1 (MQ=255)    ttGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAATCTTTCCTGCACAATGCCTTTTGACTCATGACAGGAATGGCGTTCAGGATCGATGCGATTCGACAGCGACTCGCgg                                                                                                        >  1:242466/1‑133 (MQ=255)     tGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAATCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATTCGGCTCGACAGCATCTCGCGGa                                                                                                       >  1:216377/1‑133 (MQ=255)      gCAAGGAAGGCTGCATACTCTGAAACTCAGTTATCTGTGGTTTGACTACCTGCAATTTTTCCTGCATAATGCTTTTTGTCTACTGCCACGTATGGTGTTCAGCATCGAAGGGGTTCGACAGCAACTCGCGGAt                                                                                                      <  2:477750/133‑1 (MQ=255)      gCAAGGAACGCTGCATACTCTGCACCTCCGCTATCCGTGCTTCGACTTCCTGCAATCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGTTCGACAGCAACTCGCGGAt                                                                                                      <  2:178528/133‑1 (MQ=255)      gCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAATCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGTTCGACAGCAACTCGCGGAt                                                                                                      <  1:477165/133‑1 (MQ=255)      gCAAGGAACGCTGCACACTCTGCAACTCTGCTGTCCGTGCTTCGACTTCCTGCAATCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGTTCGACAGCAACTCGCGGAt                                                                                                      <  2:222069/133‑1 (MQ=255)       cAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAATCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGTTCGACAGCAACTCGCGGATc                                                                                                     <  1:417180/133‑1 (MQ=255)           gAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAATCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGTTCGACAGCAACTCGCGGATCGACt                                                                                                 >  2:452026/1‑133 (MQ=255)            aaCGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAATCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGTTCGACAGCAACTCGCGGATCGACTc                                                                                                >  2:302900/1‑133 (MQ=255)             aCGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAATCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGTTCGACAGCAACTCGCGGATCGACTcc                                                                                               <  2:371300/133‑1 (MQ=255)                         ctGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAATCTTTCCTGCACAATGCCTTTTGGCTCCTGACAGGTATGGAGTTCAGGATCGATGCGGTTCGACAGCAACTCGCGGATCGACTCCAGACTGAAACCt                                                                                   >  2:295007/1‑133 (MQ=255)                           gCAACTCGGCTATCCGTGCTTCGACTTCCTGCAATCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGTTCGACAGCAACTCGCGGATCGACTCCAGACTGAAACCTAg                                                                                 <  2:376459/133‑1 (MQ=255)                             aaCTCGGCTATCCGTGCTTCGACTTCCTGCAATCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGTTCGACAGCAGCTCGCGGGTCGACTCCAGACTGAAACCTAGtt                                                                               >  2:154767/1‑133 (MQ=255)                             aaCTCGGCTATCCGTGCTTCGACTTCCTGCAATCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGTGCGACAGCAACTCACGGGTCGACTCCCGACTGAAACCTAGtt                                                                               >  2:164502/1‑133 (MQ=255)                                            gCTTCGACTCCCTGCTATCTTTCCTGCAAAATTCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGTTCGACAGCAACTCGCGGATCGACTCCAGACTGAAACCTAGTTGTCTGGCATGGCGGa                                                                <  2:236844/133‑1 (MQ=255)                                                      ccTGCAATCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGTTCGACAGCAACTCGCGGATCGACTCCAGACTGAAACCTAGTTGTCTGGCATGGCGGATAAATTTCaa                                                      <  1:269291/133‑1 (MQ=255)                                                                     gCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGTTCGACAGCAACTCGCGGATCGACTCCAGACTGAAACCTAGTTGTCTGGCATGGCGGATAAATTTCAATCGCTGGAGATCGCt                                       <  1:515143/133‑1 (MQ=255)                                                                       acaATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGTTCGACAGCAACTCGCGGATCGACTCCAGACTGAAACCTAGTTGTCTGGCATGGCGGATAAATTTCAATCGCTGGAGATCGCttt                                     <  1:18404/133‑1 (MQ=255)                                                                       acaATGCCTTTTGACACCTGACAGCTGTTGTGTTCAGGATCAATGCGGTTGGACAGCAACTCGCGGATCGACTCCAGACTGAAACCTAGTTGTCTGGCATGGCGGATAAATTTCAATCGCTGGAGATCGCttt                                     <  2:242466/133‑1 (MQ=255)                                                                         aaTGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGTTCGACAGCAACTCGCGGATCGACTCCAGACTGAAACCTAGTTGTCTGGCATGGCGGATAAATTTCAATCGCTGGAGATCGc                                        <  2:398948/128‑1 (MQ=255)                                                                         aaTGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGTTCGACAGCAACTCGCGGATCGACTCCAGACTGAAACCTAGTTGTCTGGCATGGCGGATAAATTTCAATCGCTGGAGATCGc                                        >  1:398948/1‑128 (MQ=255)                                                                         aaTGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGTTCGACAGCAACTCGCGGATCGACTCCAGACTGAAACCTAGTTGTCTGGCATGGCGGATAAATTTCAATCGCTGGAGATCGCTTTCg                                   <  1:54318/133‑1 (MQ=255)                                                                         aaTGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGAGCGATGCGGTTCGACAGCAACTCGCGGATCGACTCCAGACGGAAACCTAGTTGACTGGCATGGCGGATAAATTTCAATTGCTCGAGAACCCttttg                                   >  2:321227/1‑131 (MQ=255)                                                                           tGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGTTCGACAGCAACTCGCGGATCGACTCCAGACTGAAACCTAGTTGTCTGGCATGGCGTATATATTTCAATCGCTGGAGACCGCTTTCGGt                                 >  2:160668/1‑133 (MQ=255)                                                                                           aCAGGTATGGTGTTCAGGATCGATGCGGTTCGACAGCAACTCGCGGATCGACTCCAGACTGAAACCTAGATGTCTGGCATGGCGGATAAATTTCAATCGCTGGAGATCGCATTCAGTGTATAGGCGAAACCCa                 >  1:169952/1‑133 (MQ=255)                                                                                              tgTATGGTGTTCAGGCACGATGCGGTTGGACACCAACTGGCGGATCGACTCCAGACTGAAACCTAGTTGTATGGCATGGCGGATAAATTTCAATCGCTGGACATCGCTTTCGGTATATAGGCGGAACCCCCCt              <  2:212108/132‑1 (MQ=255)                                                                                                    gtgtTCAGGATCGATGCGGTTCGACAGCAACTCGCGGATCGACTCCAGACTGAAACCTAGTTGTCTGGCATGGCGGATAAATTTCAATCGCAGGAGGTCGCTTTGGGTATATAGGCGGAACCGACCTTCAgca        >  2:162303/1‑131 (MQ=255)                                                                                                      gtTCAGGAGCGATGCTGTACGAAAGCAACTCGCGGATCGACTCCAGACTGAAACCTAGTTGTCTGGCATGGCGGATAAATTTCAATCGCTGGAGATCGCTTTCGGTATATAGGCGAAACCCACCTTCAGTACg      <  1:239307/133‑1 (MQ=255)                                                                                                       ttCAGGATCGATGCGCTTTGACCGCAACTCTCGGATCGACTCCAGACTGAAACCTAGTTGTCTGGCATGGCGGATAAATTTCAATCGCTGGAGATCGCTTTCGGTATATAGGCGAAACCCACCTTCAGTACGc     <  1:160668/133‑1 (MQ=255)                                                                                                          aGGATCGATGCGGTTCGACAGCAACTCGCGGATCGACTCCAGACTGAAACCTAGTTGTCTGGCATGGCGGATAAATTTCAATCGCTGGAGAGCGCTTTCGATATATAGGCGAAACCCGCCTTGAGTACGCACt  >  2:211164/1‑133 (MQ=255)                                                                                                                       |                                                                                                                        GCGTTGCAAGGAACGCTGCATACTCTGCAACTCGGCTATCCGTGCTTCGACTTCCTGCAATCTTTCCTGCACAATGCCTTTTGACTCCTGACAGGTATGGTGTTCAGGATCGATGCGGATCGACAGCAACTCGCGGATCGACTCCAGACTGAAACCTAGTTGTCTGGCATGGCGGATAAATTTCAATCGCTGGAGATCGCTTTCGGTATATAGGCGAAACCCACCTTCAGTACGCACT  >  NC_000913/3438804‑3439041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑