Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,505,168	A→G	F285L (TTT→CTT)	yhfS ←	putative aminotransferase YhfS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,505,168	0	A	G	100.0%	66.7 / NA	20	F285L (TTT→CTT)	yhfS	putative aminotransferase YhfS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/11);  total (9/11)

CGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCAC  >  NC_000913/3505041‑3505279                                                                                                                                |                                                                                                                cGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAGCTCGa                                                                                                            <  1:116799/133‑1 (MQ=255)       gTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTCCGACTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAGCTCGACAATCa                                                                                                      <  1:2578/133‑1 (MQ=255)                  gtgATAAAAAGGCGGTGGGATTTTATTTTTCGTCTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAGCTCGACAATCAACACCTTCGAc                                                                                           <  2:244921/131‑1 (MQ=255)                    gATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAGCTCGACAATCAACACCTTCGACTg                                                                                         <  2:67372/133‑1 (MQ=255)                      tAAAAGAGCGGCGGGCTTTCATATCTCGACTCGGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAGCTCGACAATCAACACCTTCGACTgcg                                                                                       <  2:205100/133‑1 (MQ=255)                                                     ctGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAGCTCGACAATCAACACCTTCGACTGCGCAATAGCAATCACCGAGCTTTTCACTTCCgg                                                        >  2:186844/1‑133 (MQ=255)                                                        cacccaccGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAGCTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGCTTTTCACTTCCGgcac                                                     >  2:512167/1‑133 (MQ=255)                                                             accGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAGCTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGCTTTTCACTTCCGgcaccgcac                                                >  2:384676/1‑133 (MQ=255)                                                             accGGGTAAGGCAAGCCACCGCGCCTTTTCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAGCTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGCTTTTCACTTCCGgcaccgcac                                                <  2:151470/133‑1 (MQ=255)                                                               cGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAGCTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGCTTTTCACTTCCGGCACCGcacca                                              <  1:91282/133‑1 (MQ=255)                                                                        caaggcaCCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAGCTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGCTTTTCACTTACGGGACAGCACCAACGTTAAGc                                     >  1:205025/1‑133 (MQ=255)                                                                         aaggcaCCGCCCTTTAGCGCCTCTGCCAGCACTCTGGCGGCCATCGGCTGATGAAGCTCGACAATCAACACCTTCGACTGCGCATTAGCAATCCCCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCa                                    <  2:183961/133‑1 (MQ=255)                                                                            gcaCCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAGCTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAg                                 >  2:1178/1‑133 (MQ=255)                                                                              aCCGCGCTGTTGCGCTTCTTCCAGCACTCTCGCGGCAATCGGCTGATGAAGCTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGcc                               <  1:236048/133‑1 (MQ=255)                                                                                    cTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAGCTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAAc                         <  1:449697/133‑1 (MQ=255)                                                                                               ctTCCAGCACTCTGGCGGCAATCGGCTGATGAAGCTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATAc              >  2:270162/1‑133 (MQ=255)                                                                                                ttCCAGCACTCTGGCAGCCATCTGCTGATGTAGCTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATAcc             <  1:224282/133‑1 (MQ=255)                                                                                                 tCCAGCACTCTGGCGGCAATCGGCTGATGAAGCTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATAccc            >  2:28942/1‑133 (MQ=255)                                                                                                        ctctGGCGGCAATCGGCTGATGAAGCTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCcctgcctg     <  1:384676/133‑1 (MQ=255)                                                                                                          ctGGCGGCAATCGGCTGATGAAGCTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCa   >  1:474228/1‑133 (MQ=255)                                                                                                           tGGCGGCAATCGGCTGATGAAGCTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCAc  >  2:37521/1‑133 (MQ=255)                                                                                                                                |                                                                                                                CGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCAC  >  NC_000913/3505041‑3505279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑