Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	15,231	A→G	H355R (CAC→CGC)	dnaJ →	chaperone protein DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	15,231	0	A	G	100.0%	87.8 / NA	26	H355R (CAC→CGC)	dnaJ	chaperone protein DnaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/15);  total (11/15)

GGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTT  >  NC_000913/15110‑15349                                                                                                                          |                                                                                                                       ggTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCcgc                                                                                                             >  2:311634/1‑133 (MQ=255)     gcgcACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTc                                                                                                         <  2:398009/133‑1 (MQ=255)             ggTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGct                                                                                                 >  2:476169/1‑133 (MQ=255)                    tGCTGTGCCGGGTTGTCGTCGAAACACCCGTAAGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGa                                                                                          <  2:238492/133‑1 (MQ=255)                       tgtgCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATgg                                                                                       <  1:414674/133‑1 (MQ=255)                         tgCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCGCAGCAGCCCGTGCTCAAAGGGCTTGTTTGATGgtg                                                                                     >  2:144222/1‑133 (MQ=255)                         tgCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGgtg                                                                                     >  1:6471/1‑133 (MQ=255)                         tgCCGCGTTGTCGTCCAAACACCGGTAGTCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGgtg                                                                                     <  2:187494/133‑1 (MQ=255)                                             acCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTgacgac                                                                 >  1:269381/1‑133 (MQ=255)                                                 gTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGa                                                             >  1:275226/1‑133 (MQ=255)                                                    ggCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGAccc                                                          >  2:90516/1‑133 (MQ=255)                                                          aaCGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCGACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAAc                                                    <  1:476169/133‑1 (MQ=255)                                                          aaCGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAAc                                                    <  2:272794/133‑1 (MQ=255)                                                            cGAAAGGCCGAAACAGCTGCTGTAAGAGCTTCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCt                                                  <  1:144222/133‑1 (MQ=255)                                                                aGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTcccc                                              >  1:69542/1‑133 (MQ=255)                                                                       aaCAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGcc                                       <  1:272366/133‑1 (MQ=255)                                                                            ctgctgCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACGTGACCCGATAACCTACCCAAATGCCTGccc                                  >  1:232507/1‑133 (MQ=255)                                                                            ctgctgCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGccc                                  <  2:6471/133‑1 (MQ=255)                                                                                 gcaagagctgcaagaAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTggg                             <  1:90516/133‑1 (MQ=255)                                                                                      agctgcaagaAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGc                        <  2:111536/133‑1 (MQ=255)                                                                                             agaAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTa                 <  2:358323/133‑1 (MQ=255)                                                                                                       ggTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGt       <  2:8289/133‑1 (MQ=255)                                                                                                           gCCCAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGt   <  2:52847/133‑1 (MQ=255)                                                                                                            cccAACCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGCGGGCAGGGCTGGGTTAAAATAGGGTGCGtt  >  1:141756/1‑133 (MQ=255)                                                                                                                aCCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAgg         <  1:481210/122‑1 (MQ=255)                                                                                                                aCCGGCGAGCGCAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGGGAGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAgg         >  2:481210/1‑122 (MQ=255)                                                                                                                          |                                                                                                                       GGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTT  >  NC_000913/15110‑15349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑