| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,714,397 | C→T | E666E (GAG→GAA) | bisC ← | biotin sulfoxide reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,714,397 | 0 | C | T | 95.8% | 72.5 / NA | 24 | E666E (GAG→GAA) | bisC | biotin sulfoxide reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (9/14); minor base A (1/0); total (10/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTTTAATTCCCTCGCTAATGACCGCTCCGGCAAGAATTTGCCCGCGTGCGTTCCACAACCGAACAGTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGCTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCG > NC_000913/3714275‑3714524 | ggTTTAATTCCCTCGCTAATGACCGCTCCGGCAAGAATTTGCCCGCGTGCGTTCCACAACCGAACAGTATCGCCATCTTGTATGCCGCGCTGCTGGGCATGGTCTGGAGGAATGGTGGCAGGATCACGATTTg > 2:159141/1‑133 (MQ=255)ggTTTAATTCCCTCGCTAATGACCGCTCCGGCAAGAATTTGCCCGCGTGCGTTCCACAACCGAACAGTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTg < 2:451491/133‑1 (MQ=255) ccTCGCTAATGACCGCTCCGGCAAGAATTTGCCCGCGTGCGTTCCACAACCGAACAGTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTGCCACCGCGTa > 1:271395/1‑133 (MQ=255) tCGCTAATGACCGCTCCGGCAAGAATTTGCCCGCGTGCGTTCCACAACCGAACAGTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTGCCACCGCGTACa > 2:53289/1‑133 (MQ=255) tCGCTAATGACCGCTCCGGCAAGAATTTGCCCGCGTGCGTTCCACAACCGAACAGTATCGCCATCTTGTATGCCACGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTGCCACCGCGTACa > 2:20383/1‑133 (MQ=255) cTAATGACCGCTCCGGCAAGAATTTGCCCGCGTGCGTTCCACAACCGAACAGTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTGCCACCGCGTACAAtt < 2:403340/133‑1 (MQ=255) aCCGCTCCGGCAAGAATTTGCCCGCGTGCGTTCCACAACCGAACAGTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTGCCACCGCGTACAATTCGCGCa < 2:113217/133‑1 (MQ=255) gCTCCGGCAAGAATTTGCCCGCGTGCGTTCCACAACCGAACAGTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTGCCACCGCGTACAATTCGCGCagag < 2:85907/133‑1 (MQ=255) cTCCGGCAAGAATTTGCCCGCGTGCGTTCCACAACCGAACAGTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTGCCACCGCGTACAATTCGCGCAgaga > 2:420326/1‑133 (MQ=255) tCCGGCAAGAATTTGCCCGCGTGCGTTCCACAACCGAACAGTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTGCCACCGCGTACAATTCGCGCAGAGaa < 2:419103/133‑1 (MQ=255) gCCCGCGTGCGTTCCACAACCGAACAGTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTa < 2:103900/124‑1 (MQ=255) gCCCGCGTGCGTTCCACAACCGAACAGTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTa > 1:103900/1‑124 (MQ=255) cccGCGTGAGTTCCACAAGCGACCAGTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAgctgg < 1:221598/133‑1 (MQ=255) aaCAGTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGa < 1:409359/133‑1 (MQ=255) aaCAGTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGa > 2:409359/1‑133 (MQ=255) gTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATggg < 1:6221/133‑1 (MQ=255) cATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAg < 1:420326/133‑1 (MQ=255) tCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGCTGCACCAGATGGGCAGAAAGTa > 1:246171/1‑133 (MQ=255) ttGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTAcc < 1:273564/133‑1 (MQ=255) ttGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTAcc < 2:54556/133‑1 (MQ=255) cgcTCCTGGGCATCGTCAGGATGAATGGTGACAGGTTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGtt > 1:501730/1‑133 (MQ=255) aTGAATGGTGACAGGTTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGc < 1:20383/133‑1 (MQ=255) gACAGGTTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTcc > 2:295907/1‑133 (MQ=255) aCAGGTTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCg < 2:36253/133‑1 (MQ=255) | GGTTTAATTCCCTCGCTAATGACCGCTCCGGCAAGAATTTGCCCGCGTGCGTTCCACAACCGAACAGTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGCTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCG > NC_000913/3714275‑3714524 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |