Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,723,631	T→C	E256G (GAG→GGG)	glyS ←	glycine‑‑tRNA ligase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,723,631	0	T	C	100.0%	39.1 / NA	13	E256G (GAG→GGG)	glyS	glycine‑‑tRNA ligase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/2);  total (11/2)

CATACACCGGGAAGTATTTCTGGTCACCTTTCATGGTGTAAACCAGCGCTTCAGCCGGCACCGCGAGGAATTTCTCTTCGAATTTTGCGGTCAGAACGACCGGCCACTCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGTTCTTCGTAATCGGCGATGACT  >  NC_000913/3723524‑3723756                                                                                                            |                                                                                                                              cATACACCGGGAAGTATTTCTGGTCACCTTTCATGGTGTAAACCAGCGCTTCAGCCGGCACCGCGAGGAATTTCTCTTCGAATTTTGCGGTCAGAACGACCGGCCACCCCACCAGCGAAGCCACTTCTTCcag                                                                                                      >  1:361108/1‑133 (MQ=255)        cGGGAAGTATTTCTGGTCACCTTTCATGGTGTAAACCAGCGCTTCAGCCGGCACCGCGAGGAATTTCTCTTCGAATTTTGCGGTCAGAACGACCGGCCACCCCACCAGCGCAGCCACTTCTTCCAGCAGGCtt                                                                                               >  1:178444/1‑133 (MQ=255)              gTATTTCTGGTCACCTTTCATGGTGTAAACCAGCGCTTCAGCCGGCACCGCGAGGAATTTCTCTTCGAATTTTGCGGTCAGAACGACCGGCCACCCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCtt                                                                                         >  1:366398/1‑133 (MQ=255)              gTATTTCTGGTCACCTTTCATGGTGTAAACCAGCGCTTCAGCCGGCACCGCGAGGAATTTCTCTTCGAATTTTGCGGTCAGAACGACCGGCCACCCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCtt                                                                                         <  2:361108/133‑1 (MQ=255)                         cACCTTTCATGGTGTAAACCAGCGCTTCAGCCGGCACCGCGAGGAATTTCTCTTCGAATTTTGCGGTCAGAACGACCGGCCACCCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGtt                                                                              >  1:430755/1‑133 (MQ=255)                         cACCTTTCATGGTGTAAACCAGCGCTTCAGCCGGCACCGCGAGGAATTTCTCTTCGAATTTTGCGGTCAGAACGACCGGCCACCCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGtt                                                                              >  2:388485/1‑133 (MQ=255)                                         aaCCAGCGCTTCAGCCGGCACCGCGAGGAATTTCTCTTCGAATTTTGCGGTCAGAACGACCGGCCACCCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTAcgc                                                              >  1:385539/1‑133 (MQ=255)                                               cgcTTCAGCCGGCACCGCGAGGAATTTCTCTTCGAATTTTGCGGTCAGAACGACCGGCCACCCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCgctgct                                                        >  1:30135/1‑133 (MQ=255)                                               cgcTTCAGCCGGCACCGCGAGGAATTTCTCTTCGAATTTTGCGGTCAGAACGACCGGCCACCCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCgctgct                                                        >  1:350931/1‑133 (MQ=255)                                               cgcTTCAGCCGGCACCGCGAGGAATTTCTCTTCGAATTTTGCGGTCAGAACGACCGGCCACCCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCgctgct                                                        >  1:44810/1‑133 (MQ=255)                                               cgcTTCAGCCGGCACCGCGAGGAATTTCTCTTCGAATTTTGCGGTCAGAACGACCGGCCACCCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCgctgct                                                        >  1:51185/1‑133 (MQ=255)                                                                        ttCTCTTCGAATTTTGCGGTCAGAACGACCGGCCACCCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCg                               >  1:384866/1‑133 (MQ=255)                                                                                                     cGGCCACCCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGTTCTTCGTAATCGGCGATGACt  <  2:384866/133‑1 (MQ=255)                                                                                                            |                                                                                                                              CATACACCGGGAAGTATTTCTGGTCACCTTTCATGGTGTAAACCAGCGCTTCAGCCGGCACCGCGAGGAATTTCTCTTCGAATTTTGCGGTCAGAACGACCGGCCACTCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGTTCTTCGTAATCGGCGATGACT  >  NC_000913/3723524‑3723756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑