Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,790,817	T→C	I177V (ATT→GTT)	tdh ←	threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,790,817	0	T	C	100.0%	34.8 / NA	12	I177V (ATT→GTT)	tdh	threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/6);  total (6/6)

GTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAG  >  NC_000913/3790704‑3790920                                                                                                                  |                                                                                                        gTTACCCGCACGGGTGATACCCTTTTTACGCGCCACCTCAAGGCGGTTTTCGTTAACATCAGTGACCCCCACTTTGCGTGCCCCACCGTGTTCCGCCACCGCCGCTGCCATAACACCAATCGGGCCTGCACCa                                                                                      <  2:80942/133‑1 (MQ=255)                 aTACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAACACCAATCGGGCCTGCACCAGAAACCAGCACATCtt                                                                      >  1:308839/1‑133 (MQ=255)                        ttttACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAACACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCccac                                                               >  2:76024/1‑133 (MQ=255)                          ttACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAACACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCccacca                                                             <  1:29631/133‑1 (MQ=255)                          ttACCCGCAACCCCAAGGGGGTATTCGTTAACATCATTGATTCCCCTATTGATTGCACGAGCTGGTTTGGCCACCGCCGCTGCCATATCGCCAAACGGACCTTCACCAGAAACCAGCACATCTTCGCccacca                                                             <  2:93799/133‑1 (MQ=255)                                               tATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAACACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTAt                                        >  2:360588/1‑133 (MQ=255)                                                         cATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAACACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACAAGAGCAAACGACAGCGTGGTATGCACGGCGtt                              >  2:173727/1‑133 (MQ=255)                                                              gTGATCCCTACATTGTGTCCACGAACGTGTTTCGCCACCGCCGCTGCCATAACACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGa                         <  1:226120/133‑1 (MQ=255)                                                                    accacATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAACACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACTGCGCGGTGTGCGCGGCGTTACAGAAGGGAt                   >  1:136544/1‑133 (MQ=255)                                                                     ccacATTGCGTGCACCACGGTGTTTCCCCACCGCCGCTGCCATAACACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATc                  <  2:308839/133‑1 (MQ=255)                                                                            gggTGCACCACCGTGCTCCGCCACTGCCACTGTCATAACACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAAtt           <  2:136544/131‑1 (MQ=255)                                                                                gCACCAACGTGTTTCGCCACCGCCGCTGCCATAACACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAACGCGGTATACACTGCGTTACCGAAGCGATCGAAAATTGCgg       >  2:208407/1‑133 (MQ=255)                                                                                     aaCGTGTTACCCCACAGCAGCTCCCATAACTCCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAg  <  1:208407/133‑1 (MQ=255)                                                                                                                  |                                                                                                        GTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAG  >  NC_000913/3790704‑3790920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑