Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,810,369	A→G	F262L (TTT→CTT)	mutM ←	DNA‑formamidopyrimidine glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,810,369	0	A	G	100.0%	71.1 / NA	22	F262L (TTT→CTT)	mutM	DNA‑formamidopyrimidine glycosylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/12);  total (10/12)

GATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTCACCTTTTCGCCCGTAAACCTGCAATTCCTGAGCGAAATAGCCCGG  >  NC_000913/3810245‑3810474                                                                                                                             |                                                                                                          gATGTCACACATTTCCTGCCGGGGAATGTCAATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGccc                                                                                                   <  1:206077/133‑1 (MQ=255)  aTGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCg                                                                                                  <  1:95568/133‑1 (MQ=255)  aTGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCg                                                                                                  <  1:507484/133‑1 (MQ=255)             ttCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCGCTGCGCATGtt                                                                                       >  2:444986/1‑133 (MQ=255)             ttCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTACCGGCAATAAAGCGTTACCCGCTGCGCATGtt                                                                                       >  2:189992/1‑133 (MQ=255)                 tGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCGCTGCGCATGTTTAGt                                                                                   >  2:404323/1‑133 (MQ=255)                   ccGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCGCTGCGCATGTTTAGTCg                                                                                 >  2:108898/1‑133 (MQ=255)                    cGGAGAATATCCATCAGGGGCTTATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCGCTGCGCATGTTTAGTCGc                                                                                <  1:185435/133‑1 (MQ=255)                               cATCAGGCCCTGATGGCGAAGTCAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCg                                                                     <  1:152492/133‑1 (MQ=255)                                                gAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCGCTGCGCATGTTTAGTGGCCACAAGCGGTGTGCCGCACTCCCGacac                                                    >  1:197860/1‑133 (MQ=255)                                                   gTTAGCGTAGTGTTTGTGCCGGATAGTATGCCATCCGGCGCCCATGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGc                                                 <  2:197860/133‑1 (MQ=255)                                                   gTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGc                                                 <  2:83588/133‑1 (MQ=255)                                                              gtTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTCACCTTTTCg                                      >  1:267141/1‑133 (MQ=255)                                                                        gATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTCACCTTTTCGCCCGTAAAcc                            >  2:453490/1‑133 (MQ=255)                                                                        gATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTCACCTTTTCGCCCGTAAAcc                            >  2:54559/1‑133 (MQ=255)                                                                         aTGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTCACCTTTTCGCCCGTaaa                              <  1:289504/130‑1 (MQ=255)                                                                         aTGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTCACCTTTTCGCCCGTaaa                              >  2:289504/1‑130 (MQ=255)                                                                                         cgcgcATGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACATGGCTCACCCTTTCGCCCTTAAACCTGCAATTACTGAGCGaa           >  2:245253/1‑133 (MQ=255)                                                                                          gcgcATGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTCACCTTTTCGCCCGTAAACCTGCAATTCCTGAGCGaaa          <  1:108898/133‑1 (MQ=255)                                                                                               tGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTCACCTTTTCGCCCGTAAACCTGCAATTCCTGAGCGAAATAGcc     <  1:264199/133‑1 (MQ=255)                                                                                               tGAATTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTCACCTTTTCGCCCGTAAACCTGCAATTCCTGAGCGAAATAGcc     <  2:322984/133‑1 (MQ=255)                                                                                                  aTTACTTCTGGCACTGCCGACAATAAAGCGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTCACCTTTTCGCCCGTAAACCTGCAATTCCTGAGCGAAATAGCCCgg  <  1:54559/133‑1 (MQ=255)                                                                                                                             |                                                                                                          GATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTCACCTTTTCGCCCGTAAACCTGCAATTCCTGAGCGAAATAGCCCGG  >  NC_000913/3810245‑3810474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑