Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,835,367	A→G	V188A (GTC→GCC)	yicJ ←	putative xyloside transporter YicJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,835,367	0	A	G	100.0%	152.3 / NA	45	V188A (GTC→GCC)	yicJ	putative xyloside transporter YicJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/21);  total (24/21)

GGTCGTTTTGCCAGATATCACGTAAATCTTCCCGCATAGACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGA  >  NC_000913/3835245‑3835494                                                                                                                           |                                                                                                                                ggTCGTTTTGCCAGATATCACGTAAATCTTCCCGCATAGACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATAcc                                                                                                                       <  1:22598/133‑1 (MQ=255) ggTCGTTTTGCCAGATATCACGTAAATCTTCCCGCATAGACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATAcc                                                                                                                       <  1:431375/133‑1 (MQ=255)  gTCGTTTTGCCAGATATCACGTAAATCTTCCCGCATAGACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCg                                                                                                                      >  1:452408/1‑133 (MQ=255)  gTCGTTTTGCCAGATATCACGTAAATCTTCCCGCATAGACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCg                                                                                                                      >  1:448395/1‑133 (MQ=255)         tGCCAGATATCACGTAAATCTTCCCGCATAGACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGa                                                                                                               <  1:336537/133‑1 (MQ=255)               atatCACGTAAATCTTCCCGCATAGACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGa                                                                                                         <  2:452408/133‑1 (MQ=255)               atatCACGTAAATCTTCCCGCATAGACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGa                                                                                                         <  2:434273/133‑1 (MQ=255)               atatCACGTAAATCTTCCCGCATAGACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGa                                                                                                         <  2:125252/133‑1 (MQ=255)               atatCACGTAAATCTTCCCGCATAGACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGa                                                                                                         <  2:109507/133‑1 (MQ=255)               atatCACGTAAATCTTCCCGCATAGACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGa                                                                                                         <  1:486148/133‑1 (MQ=255)               atatCACGTAAATCTGCCCGCATAAACGCTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGa                                                                                                         <  2:171657/133‑1 (MQ=255)                tatCACGTAAATCTTCCCGCATAGACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAg                                                                                                        <  2:495994/133‑1 (MQ=255)                tatCACGTAAATCTTCCCGCATAGACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAg                                                                                                        <  2:448395/133‑1 (MQ=255)                         aaTCTTCCCGCATAGACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACTGAAAGGGCTGCGATACAGCCCTGGAGACCGTGTGGTttatt                                                                                               >  2:163083/1‑133 (MQ=255)                         aaTCTTCCCGCATAGACGTTGTTGTAGGTGGAGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCACCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTttatt                                                                                               >  2:97857/1‑133 (MQ=255)                          aTCTTCCCGCATAGACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCGCTGGAAGCCGAGTGGTttatta                                                                                              >  1:164533/1‑133 (MQ=255)                               cccGCATAGACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCAcc                                                                                         >  1:422690/1‑133 (MQ=255)                                ccGCATAGACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCACCCTGGAAACCAAGCGGTTTAATATCACCg                                                                                        >  1:185893/1‑133 (MQ=255)                                    atagttgttgttTTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCCCCGCGGAAAGGGCCGCGATACCGCCCTGGAAACGGAGTGGTTTATTATCACCGCCaa                                                                                    <  2:51555/127‑1 (MQ=255)                                    aTAGACGTTGTTGTATGTGGTGCTACAATGCGCTCTTTAGTGGTGAAAAAACAAAATCCCAGCATCATGAGTCCCTCCCCGGAAAGGGTAGCGATTCTGCCAGGGAAACCGAGGGGTTTATTATCACCGCCaa                                                                                    <  2:186291/133‑1 (MQ=255)                                    aTAGACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCaa                                                                                    >  1:16718/1‑133 (MQ=255)                                            tgttgtAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCAAGTGGTTCATTATCACCGCCacttaaatta                                                                            >  1:186291/1‑133 (MQ=255)                                             gttgtAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAattaaattaa                                                                           >  1:108414/1‑133 (MQ=255)                                                gtAGGTGGTGCTTCAGCGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCa                                                                        <  2:108414/133‑1 (MQ=255)                                                gtAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCa                                                                        <  2:345986/133‑1 (MQ=255)                                                     tggtgCTTCAGCGCGTTCTTTAGTGGTGAAGAAACAAAATGTCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGc                                                                   <  2:216258/133‑1 (MQ=255)                                                     tggtgCTTCAACGCGTTCTTTATTGGTGAACAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGc                                                                   <  1:163083/133‑1 (MQ=255)                                                     tggtgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCCCCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGc                                                                   <  2:397766/133‑1 (MQ=255)                                                     tggtgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGc                                                                   >  1:813/1‑133 (MQ=255)                                                       gtgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGCGGTTTCTTAACACTGCCAATTAAATTACCCAGTGGcat                                                                 >  2:207099/1‑133 (MQ=255)                                                        tgCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGcatc                                                                >  2:474840/1‑133 (MQ=255)                                                          cTTCAACGCGTTCTTTAGTGGTGATGAACCAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGcatcat                                                              <  2:217184/133‑1 (MQ=255)                                                          cTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATTACCGCCATTTAAATAAACCAGTGGcatcat                                                              >  1:231358/1‑133 (MQ=255)                                                               aCGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGaa                                                         <  1:442640/133‑1 (MQ=255)                                                               aCGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGaa                                                         <  2:16718/133‑1 (MQ=255)                                                                          aGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGc                                              >  2:476918/1‑133 (MQ=255)                                                                             ggtgAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAg                                                   >  1:501521/1‑125 (MQ=255)                                                                             ggtgAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAg                                                   <  2:501521/125‑1 (MQ=255)                                                                              gtgAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGc                                          >  1:40920/1‑133 (MQ=255)                                                                                 aagaaACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTc                                       >  1:256420/1‑133 (MQ=255)                                                                                 aagaaACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTAGCATCATCAGAACACTAGAAACCATGCCTc                                       >  1:194420/1‑133 (MQ=255)                                                                                      aCAAAATGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCcgcg                                  >  2:284431/1‑133 (MQ=255)                                                                                         aaaTGCCAGCATCATGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCTGTGGCATCCTCAGAACAGTAGAAAGTATGCCTCCCGCgggc                               >  2:249337/1‑131 (MQ=255)                                                                                                       tGAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAACCATGCCTACCGAGGGCGCCAGCACAAAACg                 >  1:165185/1‑133 (MQ=255)                                                                                                        gAATGCCACCACGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCAAGCCCCCCGCGGTTGCCACCACAAAACGc                >  2:192858/1‑133 (MQ=255)                                                                                                               accacGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGAtt         >  2:462022/1‑133 (MQ=255)                                                                                                                  acGGAAAGGGCCGCGCTACCGCCCCGGATACCCAGTGGTTTTTTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCa      <  2:143807/133‑1 (MQ=255)                                                                                                                   cGGAAAGGGCCGCGATACCGCCCTGGAAACCGAGGGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGGGGTCGCCAGCAGAAGACGCCAAGATTGCAg     >  1:149182/1‑133 (MQ=255)                                                                                                                      aaaGGGCCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCga  >  1:364555/1‑133 (MQ=255)                                                                                                                           |                                                                                                                                GGTCGTTTTGCCAGATATCACGTAAATCTTCCCGCATAGACGTTGTTGTAGGTGGTGCTTCAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGA  >  NC_000913/3835245‑3835494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑