Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,880,025	T→C	D32G (GAC→GGC)	gyrB ←	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,880,025	0	T	C	100.0%	156.5 / NA	46	D32G (GAC→GGC)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/19);  total (27/19)

CTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAAT  >  NC_000913/3879904‑3880152                                                                                                                          |                                                                                                                                cTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCg                                                                                                                      <  1:85444/133‑1 (MQ=255)    tACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCg                                                                                                                   <  2:34849/133‑1 (MQ=255)    tACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCg                                                                                                                   <  2:301952/133‑1 (MQ=255)    tACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCg                                                                                                                   <  2:112565/133‑1 (MQ=255)         agacagagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGAtata                                                                                                              >  2:57054/1‑133 (MQ=255)         agacagagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGAtata                                                                                                              >  2:13577/1‑133 (MQ=255)           acagagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATataca                                                                                                            >  1:59215/1‑133 (MQ=255)              gagTTATCGGCGTGAATGGGGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCGCCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATatacatac                                                                                                         >  1:382939/1‑133 (MQ=255)                 ttATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCg                                                                                                      <  2:88582/133‑1 (MQ=255)                   aTCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCggg                                                                                                    <  2:105405/133‑1 (MQ=255)                   aTCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGGGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCggg                                                                                                    >  2:494662/1‑133 (MQ=255)                          tGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCTTGTCGCCCATAGACCTACCCGAGCGCTTAc                                                                                             >  1:215645/1‑133 (MQ=255)                           gAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGGGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACg                                                                                            >  1:327801/1‑133 (MQ=255)                                  gACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCa                                                                                     >  1:18973/1‑133 (MQ=255)                                   aCGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCAt                                                                                    >  2:425858/1‑133 (MQ=255)                                      aTAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCGCCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCa                                                                                 <  2:382939/133‑1 (MQ=255)                                      aTAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCa                                                                                 >  2:199841/1‑133 (MQ=255)                                        aaTTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGc                                                                               <  1:13577/133‑1 (MQ=255)                                        aaTTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGc                                                                               <  1:12216/133‑1 (MQ=255)                                                tACAGTGACCCGCGACCGCTTCCTCGATAGAGTTATCTACCACATCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAg                                                                       <  1:145552/133‑1 (MQ=255)                                                     tGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCCTACGCACCGCAGCCAGCCCTTTCAGGACtt                                                                  >  1:232769/1‑133 (MQ=255)                                                       aCCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTg                                                                >  1:441853/1‑133 (MQ=255)                                                       aCCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACACACCGCATCCAGGCCTTGCAGGACTTTg                                                                >  2:217767/1‑133 (MQ=255)                                                               gcgcTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTgga                                                        <  1:425858/133‑1 (MQ=255)                                                               gcgcTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTgga                                                        <  2:59215/133‑1 (MQ=255)                                                                                  aTCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGAc                                     >  1:369823/1‑133 (MQ=255)                                                                                  aTCTACCACAACGAATACCATGTGTTCCTGACAGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGAc                                     <  2:232769/133‑1 (MQ=255)                                                                                      accaccTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCa                                 <  1:57054/133‑1 (MQ=255)                                                                                        caccTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAAc                               >  2:55739/1‑133 (MQ=255)                                                                                        caccTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAAc                               >  1:359685/1‑133 (MQ=255)                                                                                        caccTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAAc                               >  2:376198/1‑133 (MQ=255)                                                                                               aaTACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTtctc                        >  2:389945/1‑133 (MQ=255)                                                                                                aTACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCg                       <  2:359685/133‑1 (MQ=255)                                                                                                aTACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCg                       <  2:18973/133‑1 (MQ=255)                                                                                                aTACCATGAGGTGCAGACTGGTTCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTCTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCg                       <  1:217767/133‑1 (MQ=255)                                                                                                  aCCATGTGGTGCAGACCGGTGCTGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCt                     <  1:199841/133‑1 (MQ=255)                                                                                                     aTGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCAt                  >  2:280220/1‑133 (MQ=255)                                                                                                      tgtgGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAATAATTCGACATCAGCGTTTCTCGCTCAtt                 >  2:192952/1‑133 (MQ=255)                                                                                                      tgtgGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCAtt                 >  2:342849/1‑133 (MQ=255)                                                                                                             cAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACtt          >  2:405235/1‑133 (MQ=255)                                                                                                             cAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACtt          >  1:24686/1‑133 (MQ=255)                                                                                                                  cGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGtt     >  2:385537/1‑133 (MQ=255)                                                                                                                  cGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGtt     >  1:97585/1‑133 (MQ=255)                                                                                                                   ggTGCCGCCACCCGTGTCGCCGATGTACTTACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTa    <  2:215645/133‑1 (MQ=255)                                                                                                                     tGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAAt  >  1:401060/1‑133 (MQ=255)                                                                                                                     tGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAAt  >  1:38266/1‑133 (MQ=255)                                                                                                                          |                                                                                                                                CTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAAT  >  NC_000913/3879904‑3880152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑