Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	293,799	T→C	G40G (GGA→GGG)	yagL ←	CP4‑6 prophage; resolvase‑like catalytic domain‑containing protein YagL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	293,799	0	T	C	100.0%	213.1 / NA	62	G40G (GGA→GGG)	yagL	CP4‑6 prophage; resolvase‑like catalytic domain‑containing protein YagL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/28);  total (34/28)

CTCGATCACAACATGTTCAGGTAGAATATTAAATTTTTGGCATATCTCAGCAAGTGAGTGATGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACTCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTT  >  NC_000913/293670‑293926                                                                                                                                  |                                                                                                                                cTCGATCACAACATGTTCAGGTAGAATATTAAATTTTTGGCATATCTCAGCAAGTGAGTGATGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCg                                                                                                                              >  2:449192/1‑133 (MQ=255) cTCGATCACAACATGTTCAGGTAGAATATTAAATTTTTGGCATATCTCAGCAAGTGAGTGATGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCg                                                                                                                              >  1:17756/1‑133 (MQ=255) cTCGATCACAACATGTTCAGGTAGAATATTAAATTTTTGGCATATCTCAGCAAGTGAGTGATGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCg                                                                                                                              >  1:488033/1‑133 (MQ=255) cTCGATCACAACATGTTCAGGTAGAATATTAAATTTTTGGCATATCTCAGCAAGTGAGTGATGATCTGTAACGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCg                                                                                                                              <  2:290158/133‑1 (MQ=255)       ctcaacaTGCGCAGGGAGAATATTAAGTTTTTAGCATATCTCAGCAAGTGAGTGATGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCa                                                                                                                        <  2:238070/131‑1 (MQ=255)                ttCAGGTAGAATATTAAATTTTTGGCATATCTCAGCAAGTGAGTGATGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAattatt                                                                                                               >  2:442152/1‑133 (MQ=255)                ttCAGGTAGAATATTAAATTTTTGGCATATCTCAGCAAGTGAGTGATGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAattatt                                                                                                               >  2:339336/1‑133 (MQ=255)                ttCAGGTAGAATATTAAATTTTTGGCATATCTCAGCAAGTGAGTGATGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAattatt                                                                                                               >  1:366427/1‑133 (MQ=255)                    ggTAGAATATTAAATTTTTGGCATATCTCAGCAAGTGAGTGATGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTAtttttt                                                                                                           <  1:445776/133‑1 (MQ=255)                      tAGAATATTAAATTTTTGGCATATCTCAGCAAGTGAGTGATGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGa                                                                                                         <  2:488033/133‑1 (MQ=255)                      tAGAATATTAAATTTTTGGCATATCTCAGCAAGTGAGTGATGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGa                                                                                                         <  2:288759/133‑1 (MQ=255)                          atatTAAATTTTTGGCATATCTCAGCAAGTGAGTGATGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATAcc                                                                                                     >  1:445070/1‑133 (MQ=255)                            atTAAATTGTTGGCAAATCTCAGCAAGTGATTGATGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTg                                                                                                   <  1:174725/133‑1 (MQ=255)                                     ttGGCATATCTCAGCAAGTGAGTGATGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTc                                                                                          >  1:484292/1‑133 (MQ=255)                                     ttGGCATATCTCAGCAAGTGAGTGATGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTc                                                                                          >  1:275510/1‑133 (MQ=255)                                               tcAGCAAGTGAGTGATGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATa                                                                                <  2:366427/133‑1 (MQ=255)                                                      gtgagtgaTGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAaaca                                                                         <  1:206919/133‑1 (MQ=255)                                                      gtgagtgaTGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAaaca                                                                         <  1:449192/133‑1 (MQ=255)                                                      gtgagtgaTGATCTGTAAAGCTCTCTTGCTTTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAaaca                                                                         <  1:180564/133‑1 (MQ=255)                                                      gtgagtgaTGATCTGGAATGCTTTCTTGCTATTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAaaca                                                                         <  1:192242/133‑1 (MQ=255)                                                        gagtgaTGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAaacaac                                                                       >  2:97548/1‑133 (MQ=255)                                                          gtgaTGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCa                                                                     <  2:422070/133‑1 (MQ=255)                                                                atCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAaacaac                                                                       <  2:123923/125‑1 (MQ=255)                                                                atCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAaacaac                                                                       >  1:123923/1‑125 (MQ=255)                                                                 tCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATag                                                              >  2:430376/1‑133 (MQ=255)                                                                   tGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATagag                                                            >  2:30409/1‑133 (MQ=255)                                                                        tGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTGGCATAAAAAACTACTAAAAATCgagagaga                                                       >  1:242742/1‑133 (MQ=255)                                                                          cTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGAtt                                                     <  1:97548/133‑1 (MQ=255)                                                                            ttCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGc                                                   >  1:515069/1‑133 (MQ=255)                                                                             tCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGcc                                                  >  1:408946/1‑133 (MQ=255)                                                                             tCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGcc                                                  >  2:13377/1‑133 (MQ=255)                                                                             tCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGcc                                                  <  2:484292/133‑1 (MQ=255)                                                                             tCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGcc                                                  <  1:13377/133‑1 (MQ=255)                                                                                  cTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGa                                             <  2:398836/133‑1 (MQ=255)                                                                                       cGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGc                                                   >  2:16683/1‑122 (MQ=255)                                                                                       cGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGc                                                   <  1:16683/122‑1 (MQ=255)                                                                                       cGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACtt                                        >  1:89403/1‑133 (MQ=255)                                                                                         cTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTa                                      >  2:482691/1‑133 (MQ=255)                                                                                               cATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGt                                >  2:39847/1‑133 (MQ=255)                                                                                               cATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGt                                >  2:467916/1‑133 (MQ=255)                                                                                               cATATGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGt                                >  1:76277/1‑133 (MQ=255)                                                                                                acatGGTGTGAAAAACTGAACGCGTCTGTCGCCCCCCGGTGGCATAAATTATTTTTTGATACGTGAATAATGTCATTTTGCATAAAAAAAAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGTa                               <  2:242742/131‑1 (MQ=255)                                                                                                   tGGTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAag                            >  2:362103/1‑133 (MQ=255)                                                                                                    ggTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAGAAATAGAGTGTGATTGGCAGCGATAGTTTAGCTGGTAAagt                           >  2:138668/1‑133 (MQ=255)                                                                                                    ggTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGTa                               >  1:399406/1‑129 (MQ=255)                                                                                                    ggTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGTa                               <  2:399406/129‑1 (MQ=255)                                                                                                    ggTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAagt                           >  1:388458/1‑133 (MQ=255)                                                                                                    ggTGTAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAagt                           <  2:275510/133‑1 (MQ=255)                                                                                                     gtgtAAAACAATGAATGCCTCTCATGCACCTCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAagta                          <  1:157666/133‑1 (MQ=255)                                                                                                      tgtAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTgg                                 >  2:415838/1‑125 (MQ=255)                                                                                                      tgtAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTgg                                 <  1:415838/125‑1 (MQ=255)                                                                                                      tgtAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAagtag                         >  2:84579/1‑133 (MQ=255)                                                                                                      tgtAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAagtag                         >  1:122422/1‑133 (MQ=255)                                                                                                       gtAAAAAAATGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCAATACTTAAACTGGAAAagtagt                        >  1:184453/1‑133 (MQ=255)                                                                                                         aaaaaaaTGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTAt                      >  2:340914/1‑133 (MQ=255)                                                                                                         aaaaaaaTGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTACCAGCGAAACTATATCTGGTACAGTAGTAt                      >  1:236107/1‑133 (MQ=255)                                                                                                             aaaTGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTa                  <  2:26611/133‑1 (MQ=255)                                                                                                             aaaTGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTa                  <  1:482691/133‑1 (MQ=255)                                                                                                               aTGAACGCTTCTGATCCACCCCCGTGTCATCAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTAcc                <  1:181498/133‑1 (MQ=255)                                                                                                               aTGAACGCATCTGATGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTAcc                <  2:17756/133‑1 (MQ=255)                                                                                                                             tGCACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAAttgttt  <  2:76277/133‑1 (MQ=255)                                                                                                                               cACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAAttgt    <  2:478842/129‑1 (MQ=255)                                                                                                                               cACCCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAAttgt    >  1:478842/1‑129 (MQ=255)                                                                                                                                  |                                                                                                                                CTCGATCACAACATGTTCAGGTAGAATATTAAATTTTTGGCATATCTCAGCAAGTGAGTGATGATCTGTAATGCTTTCTTGCTGTTCGCTACTGCATATGGTGTAAAAAAATGAACGCATCTGATGCACTCCGGTGTCATAAATTATTTTTTGATACCTGAATAATGTCATTTTGCATAAAAAACAACCAAAAATAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTT  >  NC_000913/293670‑293926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑