| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,880,855 | T→C | T123A (ACT→GCT) | recF ← | DNA repair protein RecF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,880,855 | 0 | T | C | 92.9% | 37.6 / ‑3.7 | 14 | T123A (ACT→GCT) | recF | DNA repair protein RecF |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (7/6); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
GCCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCATCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTT > NC_000913/3880731‑3880967 | gccgcATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCATCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGCAAACCCtt < 1:281211/133‑1 (MQ=255)gccgcATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCATCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGCAAACCCtt < 1:81657/133‑1 (MQ=255) aTCCGGGTTCGTTGTGAAAGCATCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGCAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATg < 1:295121/133‑1 (MQ=255) aTCCGGGTTCGTTGTGAAAGCATCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGCAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATg > 2:6487/1‑133 (MQ=255) aTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGCAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGGGCCAGTTCAGCGCCCTGATGCCCGTGTGTACCGTCGATGCg > 2:141523/1‑133 (MQ=255) tCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGCAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACtt > 1:469362/1‑130 (MQ=255) tCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGCAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACtt < 2:469362/130‑1 (MQ=255) tCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGCAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGc > 1:270493/1‑133 (MQ=255) aGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGCAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGt > 1:423387/1‑133 (MQ=255) gAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGCAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCg < 1:6487/133‑1 (MQ=255) ctctTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTg > 1:340641/1‑133 (MQ=255) tcttctGTATTTGGGGCCGCCGTTGAGTAAAGCAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGt < 2:423387/133‑1 (MQ=255) tGTATTTGGGGCCGCCGTTGAGTAAAGCAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTc > 1:11410/1‑133 (MQ=255) ggggCCGCCGTTGAGTAAAGCAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCAGTCACCCTGTTTGTCTTTGGtt > 1:191101/1‑133 (MQ=255) | GCCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCATCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTT > NC_000913/3880731‑3880967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |