Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,063,466 A→G K407R (AAG→AGG)  yihN → putative transporter YihN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,063,4660AG93.5% 90.6 / ‑1.8 31K407R (AAG→AGG) yihNputative transporter YihN
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (15/14);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

ATGTTCTGCTTCAGTCTGTATGGCTACATTCTGGATTTAAATCCGGGGATTATTGGCTACAAAATCGTGTTTGGCATTATGGCCTGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCA  >  NC_000913/4063339‑4063594
                                                                                                                               |                                                                                                                                
aTGTTCTGCTGCAGTCTCTATGGCCTCATTCTGGATTTAAAACCGGGGATTATTAGATACAAAATCGTGTTTGGCATTATGGCCTGCTTCGCATTCAGTGGTGCGGTTGTTTCCGTAATGCTGGTTAGGCGTa                                                                                                                             <  2:225238/133‑1 (MQ=255)
     cTGCTTCAGTCTGTATGGCTACATTCTGGATTTAAATCCGGGGATTATTGGCTACAAAATCGTGTTTGGCATTATGGCCTGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGc                                                                                                                        <  2:345429/133‑1 (MQ=255)
         ttCAGTCTGTATGGCTACATTCTGGATTTAAATCCGGGGATTATTGGCTACAAAATCGTGTTTGGCATTATGGCCTGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAAc                                                                                                                    <  1:112366/133‑1 (MQ=255)
           cAGTCTGTATGGCTACATTCTGGATTTAAATCCGGGGATTATTGGCTACAAAATCGTGTTTGGCATTATGGCCTGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATACTGGTTAGACGTAGTAGCCAACGt                                                                                                                  >  2:174306/1‑133 (MQ=255)
             gTCTGTATGGCTACATTCTGGATTTAAATCCGGGGATTATTGGCTACAAAATCGTGTTTGGCATTATGGCCTGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTaa                                                                                                                <  2:429994/133‑1 (MQ=255)
             gTCTGTATGGCTACATTCTGGATTTAAATCCGGGGATTATTGGCTACAAAATCGTGTTTGGCATTATGGCCTGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTaa                                                                                                                >  2:207469/1‑133 (MQ=255)
               cTGTATGGCTACATTCTGGATTTAAATCCGGGGATTATTGGCTACAAAATCGTGTTTGGCATTATGGCCTGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTaata                                                                                                              <  1:260183/133‑3 (MQ=255)
                        tACATTCTGGATTTAAATCCGGGGATTATTGGCTACAAAATCGTGTTTGGCATTATGGCCTGCTTCGCATTCAGTGGTGCGGTGGTTTCCGAAATGCTGGCTAGCCGTTTTAGCCAATGTAAGAAAGAGATGc                                                                                                     >  2:184899/1‑133 (MQ=255)
                          cATTCTGGATTTAAATCCGGGGATTATTGGCTACAAAATCGTGTTTGGCATTATGGCCTGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTAAGAAAGAGATGCTg                                                                                                   >  1:335967/1‑133 (MQ=255)
                            ttCTGGATTTAAATCCGGGGATTATTGGCTACAAAATCGTGTTTGGCATTATGGCCTGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGTCGTGTTAACCAACGTAGGAAATAGATGCTggc                                                                                                 >  2:169109/1‑133 (MQ=255)
                                ggATTTAAATCCGGGCATTATTCGCTACAAAATCGTGTTTGGCATTATGGCCTGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCt                                                                                             <  1:169109/133‑1 (MQ=255)
                                 gATTTAAATCCGGGGATTATTGGCTACAAAATCGTGTTTGGCATTATGGCCTGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTg                                                                                            <  1:300149/133‑1 (MQ=255)
                                 gATTTAAAGCCGGGGATTATTGACTACAAATTCGTGTTTGGCATTATGGCCTGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTg                                                                                            <  1:184899/133‑1 (MQ=255)
                                  aTTTAAATCCGGGGATTATTGGCTACAAAATCGTGTTTGGCATTATGGCCTGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGa                                                                                           <  1:398977/133‑1 (MQ=255)
                                      aaaTCCGGGGATTATTGGCTACGAAATCGTGTTTGGCATTATGGCCTGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCt                                                                                       <  1:207469/133‑1 (MQ=255)
                                                     tGGCTACAAAGTCGTCTTTGGCATGATGGCCTTCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGa                                                                        <  2:193743/133‑1 (MQ=255)
                                                     tGGCTACAAAATCGTGTTTGGCATTATGGCCTGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGa                                                                        >  2:412746/1‑133 (MQ=255)
                                                            aaaaTCGTGTTTGGCATTATGGCCTGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCtt                                                                 >  2:370587/1‑133 (MQ=255)
                                                                              aTGGCCTGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAAc                                               <  2:335967/133‑1 (MQ=255)
                                                                                    tGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATc                                         >  2:452197/1‑133 (MQ=255)
                                                                                    tGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATc                                         >  1:307684/1‑133 (MQ=255)
                                                                                     gCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCa                                        >  1:276984/1‑133 (MQ=255)
                                                                                      cTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTACTGGCCGGACGTGCTTAATCCGGCCACCGAACACCGTATCaa                                       >  2:187412/1‑133 (MQ=255)
                                                                                           cATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTTATCCGGCCAACTAAAACCGTACCAACCGTa                                  >  1:173987/1‑133 (MQ=255)
                                                                                           cATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAAcc                                     <  1:350430/130‑1 (MQ=255)
                                                                                           cATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAAcc                                     >  2:350430/1‑130 (MQ=255)
                                                                                                aGTGGTGCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCg                             >  1:277552/1‑133 (MQ=255)
                                                                                                aGTGGTGCGGTGGTTTCCGTAAGGCTGGTTCGGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCg                             <  1:174306/133‑1 (MQ=255)
                                                                                                      gCGGTGGTTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAg                       >  2:300985/1‑133 (MQ=255)
                                                                                                          tggtTTCCGTAATGCTGGTTAGGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGt                   >  1:479938/1‑133 (MQ=255)
                                                                                                                    aaTGCTGGTTAGGCGTATTAGCCACCGTTAGACAGAGATGCAGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCa         <  2:173987/133‑1 (MQ=39)
                                                                                                                           gTTAGGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAGGGCGTTAACGCCGAATCCGGCa  >  1:146849/1‑133 (MQ=255)
                                                                                                                               |                                                                                                                                
ATGTTCTGCTTCAGTCTGTATGGCTACATTCTGGATTTAAATCCGGGGATTATTGGCTACAAAATCGTGTTTGGCATTATGGCCTGCTTCGCATTCAGTGGTGCGGTGGTTTCCGTAATGCTGGTTAAGCGTATTAGCCAACGTAAGAAAGAGATGCTGGCGGCTGAAGCTTAATTAATGGCCGGATGTGCTTAATCCGGCCAACGAAAACCGTATCAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCA  >  NC_000913/4063339‑4063594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑