Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,070,450	T→C	intergenic (‑49/+65)	yihR ← / ← yihS	putative aldose 1‑epimerase YihR/sulfoquinovose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,070,450	0	T	C	100.0%	153.3 / NA	45	intergenic (‑49/+65)	yihR/yihS	putative aldose 1‑epimerase YihR/sulfoquinovose isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/21);  total (24/21)

CCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGG  >  NC_000913/4070325‑4070578                                                                                                                              |                                                                                                                                 cccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCg                                                                                                                           <  1:95430/133‑1 (MQ=255) cccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCAGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCg                                                                                                                           <  2:346712/133‑1 (MQ=255)   cgcgGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGcc                                                                                                                         >  1:506726/1‑133 (MQ=255)   cgcgGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCACTCGcc                                                                                                                         <  2:80201/133‑1 (MQ=255)    gcgGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGccc                                                                                                                        >  2:81566/1‑133 (MQ=255)     cgGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGcccc                                                                                                                       <  2:478413/133‑1 (MQ=255)             gAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGga                                                                                                               <  1:81566/133‑1 (MQ=255)             gAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGga                                                                                                               <  1:384381/133‑1 (MQ=255)                     cTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAGCTGCACCGAACGGCCCACTCGCCCCATTGGGGAGAGGGTTa                                                                                                       >  2:195497/1‑133 (MQ=255)                               cAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAgggg                                                                                             <  2:506726/133‑1 (MQ=255)                                  tGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGaaa                                                                                          >  2:460131/1‑133 (MQ=255)                                  tGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGaaa                                                                                          >  2:362211/1‑133 (MQ=255)                                  tGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGaaa                                                                                          >  2:461998/1‑133 (MQ=255)                                        ttGGTGATCTGCATACAACGCAACTTAATTCACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGc                                                                                    <  1:148928/133‑1 (MQ=39)                                         tGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTGGCCCCTTTAGGGAGAGGGTTAGGGAAAGGGGAAAACCCGCa                                                                                   >  2:162059/1‑133 (MQ=255)                                             gATTTGCATACAAGGAACTTTACTTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAACCTGCCCCGGCCGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCt                                                                               <  1:195497/133‑1 (MQ=38)                                              aTTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGc                                                                                    >  2:3078/1‑127 (MQ=255)                                              aTTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGc                                                                                    <  1:3078/127‑1 (MQ=255)                                              aTTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTa                                                                              >  1:382402/1‑133 (MQ=255)                                                 tGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTAttt                                                                           >  1:337530/1‑133 (MQ=255)                                                   cATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTc                                                                         >  1:427585/1‑133 (MQ=255)                                                   cATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTc                                                                         >  2:351856/1‑133 (MQ=255)                                                       cAAGGCACTTTAATTTACTACAGACAGCCTACCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGAt                                                                     <  1:162059/133‑1 (MQ=38)                                                           gCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGt                                                                 <  2:382402/133‑1 (MQ=255)                                                             aCCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTa                                                               >  2:21904/1‑133 (MQ=255)                                                                  aattaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCg                                                          <  1:362211/133‑1 (MQ=255)                                                                  aattaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCg                                                          >  2:409951/1‑133 (MQ=255)                                                                  aattaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCg                                                          <  2:427585/133‑1 (MQ=255)                                                                         gACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTa                                                     >  1:358291/1‑131 (MQ=255)                                                                         gACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTa                                                     <  2:358291/131‑1 (MQ=255)                                                                         gACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAat                                                   >  2:129439/1‑133 (MQ=255)                                                                         gACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAat                                                   >  1:72811/1‑133 (MQ=255)                                                                          aCATACAGGCTCCCGTGACGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAata                                                  <  1:194129/133‑1 (MQ=255)                                                                          aCATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAata                                                  <  1:489496/133‑1 (MQ=255)                                                                           cATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTGAGCGAGATGGGAAAACCCGCACCCCATTTTTAGATCAGTTATTTCGCATTAatat                                                 >  2:366041/1‑133 (MQ=255)                                                                                  ccTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTCATTGCGCATTAATATCcagcag                                          >  1:227670/1‑133 (MQ=255)                                                                                     cccGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGAcc                                       >  1:260628/1‑133 (MQ=255)                                                                                     cccGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGAcc                                       >  2:329078/1‑133 (MQ=255)                                                                                     cccGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGAcc                                       <  1:461998/133‑1 (MQ=255)                                                                                     cccGTGAGGCTGACGTCAAACCCAAAAGCTGCACCGGCCGGCCCCCTCGCCCCGTTGGGTAGAGGCCTAGGGAGACCGGAAAACCCGACCCCCCTTTTCAGATCAGCTATTTCGCATTAATATCCAGCAGAcc                                       >  2:50780/1‑133 (MQ=255)                                                                                         tGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGGTATTTCGTATTAATATCCAGCAGACCCGCt                                   >  1:158738/1‑133 (MQ=255)                                                                                           aGGCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGc                                 <  1:329078/133‑1 (MQ=255)                                                                                            ggCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCa                                >  1:366764/1‑133 (MQ=255)                                                                                            ggCTGATGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTCCGCTTTAATATCAAGCAAACCCGCTGCa                                >  2:225379/1‑133 (MQ=38)                                                                                                 aTGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGc                           >  2:88426/1‑133 (MQ=255)                                                                                                  tGTCAAACCCAAAAACTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCt                          <  2:72811/133‑1 (MQ=255)                                                                                                           cAAAAACTGCACCGGACGGCCCCCTCGCCACTTTGGGGGGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATg                 >  2:453997/1‑133 (MQ=255)                                                                                                                aCTGCACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCggg            <  1:88426/133‑1 (MQ=255)                                                                                                                    cACCGGACGGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCt        >  2:23786/1‑133 (MQ=255)                                                                                                                          aCGGCCCCCTCGCCCCTTTGGGGAGAGGGTGAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACggg  <  1:225379/133‑1 (MQ=255)                                                                                                                              |                                                                                                                                 CCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGG  >  NC_000913/4070325‑4070578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑