| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,097,658 | T→G | intergenic (‑210/‑78) | rhaB ← / → rhaS | rhamnulokinase/DNA‑binding transcriptional activator RhaS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,097,658 | 0 | T | G | 97.7% | 137.7 / NA | 43 | intergenic (‑210/‑78) | rhaB/rhaS | rhamnulokinase/DNA‑binding transcriptional activator RhaS |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (13/29); minor base A (1/0); total (14/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGT > NC_000913/4097533‑4097785 | cATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACCCTGTATCTAGAAGAATGGACGTTTTATACGTGGGTTTCCGt > 2:145404/1‑133 (MQ=255) aTTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGACAAATCGAGGTTGTTGACGTGGGTTTCCGTc > 1:200899/1‑133 (MQ=255) aTTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTc > 1:444669/1‑133 (MQ=255) aTTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGAAGTTTTTGACGTGGATTTCCGTc > 1:192425/1‑133 (MQ=255) ccAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAAttt < 2:444669/133‑1 (MQ=255) ggAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGATTTGTACGTGGGTTTCTGTCGAAAATTTAAgg > 2:190860/1‑133 (MQ=255) aaaGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTa < 1:86708/133‑1 (MQ=255) aaaGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTa < 1:323553/133‑1 (MQ=255) aaGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTaa < 1:44600/133‑1 (MQ=255) aaCGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCt < 1:83763/133‑1 (MQ=255) gTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTATTACGTGGGTTTCCGTGGAAAATTTGAGGTGAGAACCTGAc > 2:136739/1‑133 (MQ=255) tgatgaTGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGTATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGAcc < 2:88274/133‑1 (MQ=255) gatgatGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCt > 1:338285/1‑133 (MQ=255) atgatgTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTc < 2:400179/133‑1 (MQ=255) gatgTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGt < 1:269305/133‑1 (MQ=255) gTTCACAACTTGCTGCATTGTGCTGATGTGCTGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGAt < 1:145404/133‑1 (MQ=255) tCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTa < 1:483724/133‑1 (MQ=255) tGCTGAATTGTGGTGTTGTGATGCTCACTGCATTTCCTGAATATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCg < 2:200899/133‑1 (MQ=255) tGCTGAATTGAGGTGATGTGATGCTCACTGCACTTCCTGAATATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCg < 2:192425/133‑1 (MQ=255) gCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGc > 2:307106/1‑133 (MQ=255) gCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGGAAGAACCTGACATCGTGATTAATATTTCGc > 1:166454/1‑133 (MQ=255) gtggtgATGTGATGCTCACCGCAATTTCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTGACGTGGGTTTCCGTCGAAAATTTAAGGTAAGATCCTGACCTCGTGATGACTATTTCGCCGTGTTga < 2:255465/133‑1 (MQ=255) tggtgATGTGATGCTAACCGCATTTCCTCAAAATTCACGCTTTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTgac < 2:166454/133‑1 (MQ=255) cTCACCGCTTTTCCTCAAGATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGc < 1:192753/133‑1 (MQ=255) cTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGc > 1:321289/1‑133 (MQ=255) cTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACATGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGc < 2:109154/133‑1 (MQ=255) aCCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAg > 1:487501/1‑133 (MQ=255) gCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTAt < 2:343110/133‑1 (MQ=255) aTTTCCTTAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGa < 2:453075/133‑1 (MQ=255) aTTTCCTGTAAATTCCCGCTGTCTCTTGAATAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGa < 1:136739/133‑1 (MQ=255) aTTTCCTGAAAATTCACGCTGTATTTTGCAAACTCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGa < 2:191851/133‑1 (MQ=255) aTTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGa < 2:338285/133‑1 (MQ=255) aTTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGa < 1:278712/133‑1 (MQ=255) aTTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGa < 2:278518/133‑1 (MQ=255) ttCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGAcc < 2:332030/133‑1 (MQ=255) ttCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGAcc < 1:22724/133‑1 (MQ=255) ttCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGAcc < 1:5462/133‑1 (MQ=255) ttCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGAcc < 1:269329/133‑1 (MQ=255) ttCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGAcc > 1:114917/1‑133 (MQ=255) aaaaTTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTa < 2:280501/133‑1 (MQ=255) aaTCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTc > 1:89059/1‑133 (MQ=255) aaTCGACGTTTTTTACGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTc > 2:349890/1‑133 (MQ=255) tACGTGGGTTTCCGTTGAAAATCTAAGGTACGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTcc < 1:252492/133‑1 (MQ=255) cGTGGGTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGt < 1:349890/133‑1 (MQ=255) | CATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAGGCCAGTATGACCGTATTACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGT > NC_000913/4097533‑4097785 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |