| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,103,728 | A→G | G416G (GGT→GGC) | cpxA ← | sensory histidine kinase CpxA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,103,728 | 0 | A | G | 95.2% | 52.4 / ‑4.1 | 21 | G416G (GGT→GGC) | cpxA | sensory histidine kinase CpxA |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (10/10); total (10/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01 | |||||||||||
TTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCT > NC_000913/4103601‑4103845 | tttAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGcc < 2:278503/133‑1 (MQ=255) tAACTCCGCTTATACTGCCGCATCCAAATCCCCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTGCCGCCAg < 1:148701/133‑1 (MQ=255) cGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTGCCGCCAGATTCAc > 1:273489/1‑133 (MQ=255) cTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTGCCGCCAGATTCACGa > 2:111272/1‑133 (MQ=255) cTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCCCCACCCCACAACCTGGCCCGCTCGATTCACGa > 2:207928/1‑133 (MQ=255) gCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAACCGCCCGCCCCAAACCTGTGCCGCCAGATTCACGATCGCGTGCTTc > 2:284021/1‑133 (MQ=255) aCCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGGCCCAAACCCGTGGCACCAGATTCACGATCGAGTGCTTCATc > 2:226651/1‑133 (MQ=255) aCCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTGCCGCCAGATTCACGATCGCGTGCTTCATc > 1:293218/1‑133 (MQ=255) aCCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGAGCCGCCAGATTGACAATCGCGAGCTTCATc > 1:221321/1‑133 (MQ=255) cAAATCACAAGCCGTTAACCGCTCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTGCCGCCAGATTCACGATCGCGTGCTTCATCgg < 1:207928/133‑1 (MQ=255) aaaTCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTGCCGCCAGATTCACGATCGCGTGCTTCATCGGt < 1:111272/133‑1 (MQ=255) tAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTGCCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAacgga < 1:410483/133‑1 (MQ=255) cccAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTGCCGCCAGATTCACGATCGCGGGCTTCATCGGTACGATAGAACGGACGGaaaa > 1:501138/1‑133 (MQ=255) cttctGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTGCCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATc > 1:40294/1‑133 (MQ=255) tCACCCGGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTGCCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGAACGGAAATTCTGTTGGCGATCTTCCGGGCt > 1:180839/1‑133 (MQ=255) tCACCCAGTCACGATGCTCCTGGATGGCGGCTTCAACAATCGCCAGCCCCAAACCTGTGCCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCt < 1:223273/133‑1 (MQ=255) tCACCCAGCCACGATTCTGCTGTATGGCGGGTTCAACAATCGCCAGCCCCAAACCTGTGCCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCt < 1:226651/133‑1 (MQ=255) tCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTGCCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCt < 2:273489/133‑1 (MQ=255) gCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTGCCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCTAACGCCa > 2:117790/1‑133 (MQ=255) aTGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTGCCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCTAACGCCAGGACCa < 1:117790/133‑1 (MQ=255) tgctgAATGGCGGTTTCAACAATCGCCAGCCCCAAACCCGTTCCGCCAGATTCACGATCGCGTGCTTTAGCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTTCGGGCTAACCCCAAGACCAtcct > 2:432898/1‑131 (MQ=255) gctgAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTGCCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCTAACGCCAGGACCAtcgtc > 1:289566/1‑133 (MQ=255) gAATGGCGGTTTCAACAACCGCCAGCCCCAAACCTGTGCCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCTAACGCCAGGACCATCgtcgtc < 1:284021/133‑1 (MQ=255) tGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTGCCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCAc > 1:6574/1‑133 (MQ=255) ggTTTCAACACTCGCCAGCCCCAACCCTCTGCAGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTa < 2:221321/133‑1 (MQ=255) gCCAGCCCCAAACCTGAGCCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACAGAAAATCTGTTCGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGCCCACCGTAATGGTGATACCGt > 2:443/1‑133 (MQ=255) cAGCCCCACACCTGTTCCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCt < 1:161296/133‑1 (MQ=255) | TTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCTAACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCT > NC_000913/4103601‑4103845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |