Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,281,343	A→G	E455G (GAG→GGG)	yjcE →	putative transporter YjcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,281,343	0	A	G	100.0%	85.5 / NA	27	E455G (GAG→GGG)	yjcE	putative transporter YjcE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/15);  total (12/15)

CGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGA  >  NC_000913/4281215‑4281472                                                                                                                                 |                                                                                                                                  cGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGt                                                                                                                               >  1:23287/1‑133 (MQ=255)   tggagttGTTGCCTATTCTTCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAATGCATTGCGCGAGCGGCAACGGCGGAAGTGGCGATTTTGGAGATCCAGAGAATGTTGGGGCGTCt                                                                                                                             >  2:139871/7‑133 (MQ=255)                tATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATAcc                                                                                                                >  1:457583/1‑133 (MQ=255)                       cTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGTCGGATACCGAAgaga                                                                                                         >  1:183017/1‑133 (MQ=255)                            acacATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATc                                                                                                    >  1:74450/1‑133 (MQ=255)                                aTTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAaga                                                                                                           <  2:415903/122‑1 (MQ=255)                                aTTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAaga                                                                                                           >  1:415903/1‑122 (MQ=255)                                    aaGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCa                                                                                            <  2:457583/133‑1 (MQ=255)                                          cgGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAgctgct                                                                                      >  2:352305/1‑133 (MQ=255)                                            gATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTa                                                                                    >  1:125743/1‑133 (MQ=255)                                               cATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACgg                                                                                 <  1:97934/133‑1 (MQ=255)                                                          aTTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTtct                                                                      <  2:23287/133‑1 (MQ=255)                                                           ttGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTtctc                                                                     >  1:8634/1‑133 (MQ=255)                                                               aaGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGt                                                                 <  2:74450/133‑1 (MQ=255)                                                               aaGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGt                                                                 <  2:8634/133‑1 (MQ=255)                                                               aaGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGt                                                                 <  2:310978/133‑1 (MQ=255)                                                               aaGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGt                                                                 <  2:284610/133‑1 (MQ=255)                                                               aaGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGt                                                                 <  1:352305/133‑1 (MQ=255)                                                               aaGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCAGCTTACGGAGGTCAGTTCTCGCGt                                                                 <  2:345666/133‑1 (MQ=255)                                                                     aaCGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTgg                                                           >  1:41849/1‑133 (MQ=255)                                                                         tATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAAc                                                       >  2:373983/1‑133 (MQ=255)                                                                                              aGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGa                                  <  1:373983/133‑1 (MQ=255)                                                                                                     gCGATTGTGGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAacg                           >  2:257115/1‑133 (MQ=255)                                                                                                       gatgttgGAGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAacgac                         <  1:377779/128‑1 (MQ=255)                                                                                                            tgGCGATCCAGAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGa                    <  2:398982/133‑1 (MQ=255)                                                                                                                      gAAAATGGAGGGGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTg          >  1:118336/1‑133 (MQ=255)                                                                                                                         aaTGGAGGGGCGTCTTGCGGCGGATACCTAAGCGAATATCGCTAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAg       <  2:204334/133‑1 (MQ=255)                                                                                                                         aaTGGAGGGGCGGCTCGCGGCGGAGACCGAAGCGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAg       <  2:183017/133‑1 (MQ=255)                                                                                                                            ggagggGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATCGACGTAACGACGTTGACAGTTCCGTGCAGGaa    >  2:221646/1‑133 (MQ=255)                                                                                                                              aggggCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAaga  <  2:374842/133‑1 (MQ=255)                                                                                                                                 |                                                                                                                                  CGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATGGAGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGA  >  NC_000913/4281215‑4281472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑