Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,282,253	T→C	S275G (AGC→GGC)	yjcF ←	pentapeptide repeat‑containing protein YjcF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,282,253	0	T	C	100.0%	202.2 / NA	58	S275G (AGC→GGC)	yjcF	pentapeptide repeat‑containing protein YjcF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/38);  total (20/38)

ACGTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTG  >  NC_000913/4282124‑4282381                                                                                                                                  |                                                                                                                                 aCGTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTGAATCAATTTTAGAGAAGCCAAg                                                                                                                               >  1:157989/1‑133 (MQ=255)   gTATTTGTTAACCGCGTAAAAAAGAGTTTGCTTTCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGt                                                                                                                             <  1:267290/133‑1 (MQ=255)   gTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGt                                                                                                                             <  1:349265/133‑1 (MQ=255)   gTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGt                                                                                                                             <  1:34341/133‑1 (MQ=255)        tGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGct                                                                                                                        <  2:503424/133‑1 (MQ=255)            aaCCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAACCAAGTTTTGAGCAGCCAAGGTAGGCTCtaa                                                                                                                    >  1:178926/1‑133 (MQ=255)             aCCGCGTAGAAAAGATTTTGCTTTCAATTACGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCtaaa                                                                                                                   <  1:196245/133‑1 (MQ=255)             aCCGCGTAATCAAGAGTTTGCTATTATTAAAGTTTATATTCGGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCtaaa                                                                                                                   <  2:229689/133‑1 (MQ=255)             aCCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCtaaa                                                                                                                   <  2:418783/133‑1 (MQ=255)             aCCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCtaaa                                                                                                                   <  1:378336/133‑1 (MQ=255)             aCCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCtaaa                                                                                                                   <  1:455549/133‑1 (MQ=255)             aCCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCtaaa                                                                                                                   <  1:466710/133‑1 (MQ=255)             aCCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCtaaa                                                                                                                   <  1:316786/133‑1 (MQ=255)             aCCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCtaaa                                                                                                                   <  2:303675/133‑1 (MQ=255)             aCCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCtaaa                                                                                                                   >  1:21205/1‑133 (MQ=255)             aCCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCtaaa                                                                                                                   <  1:127174/133‑1 (MQ=255)               cgcgTAAAAACGAGTATGCTATCACTTACGTTGATATTCTGCAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCtaaata                                                                                                                 <  2:133727/133‑1 (MQ=255)               cgcgTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCtaaata                                                                                                                 <  2:444573/133‑1 (MQ=255)                 cgTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGCGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAGTTAAATCAGGTTGAGAAAAGCCAAGGTCGGGTCtaaataaa                                                                                                               >  2:162484/1‑133 (MQ=255)                 cgTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCtaaataaa                                                                                                               >  1:462883/1‑133 (MQ=255)                 cgTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCtaaataaa                                                                                                               >  2:479788/1‑133 (MQ=255)                 cgTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCtaaataaa                                                                                                               >  1:353272/1‑133 (MQ=255)                  gTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCtaaa                                                                                                                   >  1:435041/1‑128 (MQ=255)                  gTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCtaaa                                                                                                                   <  2:435041/128‑1 (MQ=255)                          gagTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAatatat                                                                                                      <  1:265347/133‑1 (MQ=255)                           agTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATg                                                                                                     <  2:256163/133‑1 (MQ=255)                            gTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATgg                                                                                                    <  2:21205/133‑1 (MQ=255)                                    tCAATTAAGTTTATACTCTGGAGGATTGTTTTCTTCAGGTTTACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTa                                                                                            <  2:160005/133‑1 (MQ=255)                                     cAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTAc                                                                                           >  1:490203/1‑133 (MQ=255)                                                atatTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGAt                                                                                >  2:422272/1‑133 (MQ=255)                                                atatTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAAATAATATAAGGCGCTTTTACTTTATAAAGAt                                                                                >  1:187911/1‑133 (MQ=255)                                                  atTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGAttt                                                                              >  2:129314/1‑133 (MQ=255)                                                      tGGAGGGTTGCTTTATTCGGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCt                                                                          <  1:202119/133‑1 (MQ=255)                                                      tGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCt                                                                          >  1:470925/1‑133 (MQ=255)                                                      tGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCt                                                                          <  2:32490/133‑1 (MQ=255)                                                      tGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATCACTTTATAAAGATTTACCt                                                                          <  2:507013/133‑1 (MQ=255)                                                         aGGGTTGCTTTATTCTGGTTGACATGAGACAGATCAGCATGCTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAg                                                                       <  2:157989/133‑1 (MQ=255)                                                         aGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAg                                                                       <  2:452462/133‑1 (MQ=255)                                                         aGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAg                                                                       <  1:10649/133‑1 (MQ=255)                                                         aGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAg                                                                       <  1:479788/133‑1 (MQ=255)                                                             ttGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAg                                                                       <  1:428264/129‑1 (MQ=255)                                                             ttGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAg                                                                       >  2:428264/1‑129 (MQ=255)                                                                     ttCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCa                                                           >  1:275155/1‑133 (MQ=255)                                                                             gACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATaaaaa                                                   >  2:51976/1‑133 (MQ=255)                                                                             gACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATaaaaa                                                   >  2:461019/1‑133 (MQ=255)                                                                                  gagaCAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGa                                              <  1:35488/133‑1 (MQ=255)                                                                                             gCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCaagga                                   <  1:51976/133‑1 (MQ=255)                                                                                                       aGTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTAttttt                         <  2:490203/133‑1 (MQ=255)                                                                                                        gTTAATTAAATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTa                        >  1:376416/1‑133 (MQ=255)                                                                                                            attaaATCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAatat                    <  2:275155/133‑1 (MQ=255)                                                                                                                 aTCAAGTTTAGAGAATCCGAGGGCGGCTCTAAATAAATTAATATATGGCGCTATTGCTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGct               <  1:162484/133‑1 (MQ=255)                                                                                                                 aTCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGct               <  1:60543/133‑1 (MQ=255)                                                                                                                 aTCAAGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGct               <  2:482/133‑1 (MQ=255)                                                                                                                     aGTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCTAGGGAGGTATTTCTAATCATGGCTCTAt           >  2:237321/1‑133 (MQ=255)                                                                                                                      gTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTAtt          <  2:86022/133‑1 (MQ=255)                                                                                                                      gTTTAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTAtt          <  1:439606/133‑1 (MQ=255)                                                                                                                         tAGAGAAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCAt       <  1:12203/133‑1 (MQ=255)                                                                                                                             gaAGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCAttgtt   >  2:18950/1‑133 (MQ=255)                                                                                                                              aaGCCAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCAttgttg  <  1:461019/133‑1 (MQ=255)                                                                                                                                  |                                                                                                                                 ACGTATTTGTTAACCGCGTAAAAAAGAGTTTGCTATCAATTAAGTTTATATTCTGGAGGGTTGCTTTATTCAGGTTGACACGAGACAGATCAGCATGTTCAAAGTTAATTAAATCAAGTTTAGAGAAGCTAAGGTCGGCTCTAAATAAATTAATATATGGCGCTATTACTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTG  >  NC_000913/4282124‑4282381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑