Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,320,725	T→A	I175F (ATC→TTC)	phnH ←	carbon‑phosphorus lyase core complex subunit PhnH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,320,725	0	T	A	100.0%	48.3 / NA	16	I175F (ATC→TTC)	phnH	carbon‑phosphorus lyase core complex subunit PhnH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (9/7);  total (9/7)

GGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGC  >  NC_000913/4320612‑4320818                                                                                                                  |                                                                                              gggCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGAACAGGTCGATGCCGAGCggg                                                                            >  2:84126/1‑133 (MQ=255)   gCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGAACAGGTCGATGCCGAGCGGGaa                                                                          <  1:84126/133‑1 (MQ=255)    cGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGAACAGGTCGATGCCGAGCGGGAAc                                                                         >  1:403346/1‑133 (MQ=255)      tGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGACAGGAACAGGTAGACGCCGAGCGGGAACgg                                                                       >  1:243576/1‑133 (MQ=255)               tCGATCGCATTCTCGCCCGCTTCCACGGCATCGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGAACAGGTCGATGCCGAGCGGGAACGGATGCGGgcg                                                              <  1:162911/133‑1 (MQ=255)                             gCCCCCTTACACGGCTACATACATCAGCACCCCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGAACAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGt                                                <  1:217954/133‑1 (MQ=255)                               ccccTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGAACAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGc                                              <  2:118936/133‑1 (MQ=255)                                           cAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGAACAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTcc                                  >  1:54768/1‑133 (MQ=255)                                             aCGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGAACAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCgg                                <  1:382426/133‑1 (MQ=255)                                                        cacaCCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCGCGCCACAGGTCAGGAACAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAg                     <  2:282975/133‑1 (MQ=255)                                                          cacCTCCACCTGAGTGGTTCGCGATATACCCAGCAGGCGCTCGCCACAGGTCAGGAACAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAGCg                   <  2:243576/133‑1 (MQ=255)                                                             cTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGAACAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAGCGATc                >  1:405011/1‑133 (MQ=255)                                                                       gTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGAACAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAGCGATCATTCGttctt      >  2:511703/1‑133 (MQ=255)                                                                          gTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGAACAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAGCGATCATTCGTTCTTCgg   >  1:455559/1‑133 (MQ=255)                                                                          gTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGAACAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAGCGATCATTCGTTCTTCgg   >  1:61535/1‑133 (MQ=255)                                                                           ttCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGAACAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGc  >  1:513547/1‑133 (MQ=255)                                                                                                                  |                                                                                              GGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGCGCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGC  >  NC_000913/4320612‑4320818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑