Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,359,475	C→T	G186D (GGT→GAT)	cadB ←	lysine:cadaverine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,359,475	0	C	T	100.0%	164.5 / NA	49	G186D (GGT→GAT)	cadB	lysine:cadaverine antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/21);  total (28/21)

ACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGG  >  NC_000913/4359345‑4359600                                                                                                                                   |                                                                                                                              aCCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCa                                                                                                                             >  1:348513/1‑133 (MQ=255)  ccAGTACCCAGCATGGTTACCAGCGGAACGCTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCAt                                                                                                                            <  1:208609/133‑1 (MQ=255)      tACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGTTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGAAGACTCCTTTTAATGAACGCACGATCATCAGt                                                                                                                        >  2:230471/1‑133 (MQ=255)      tACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGt                                                                                                                        >  1:510176/1‑133 (MQ=255)       aCCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTg                                                                                                                       >  2:406882/1‑133 (MQ=255)          cAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGATTACTTTTCATGATCGCATGATTATCAGTGGTa                                                                                                                    >  2:208609/1‑133 (MQ=255)           aGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTAt                                                                                                                   >  1:115013/1‑133 (MQ=255)            gCATGGTTGCCAGCGGAACGGTACTTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATc                                                                                                                  <  2:228060/133‑1 (MQ=255)            gCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATc                                                                                                                  <  1:492031/133‑1 (MQ=255)                   tGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCCGTGGTATCCGCAGTa                                                                                                           >  2:227609/1‑133 (MQ=255)                           gAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGtt                                                                                                   >  2:494171/1‑133 (MQ=255)                            aaCGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTa                                                                                                  <  2:97783/133‑1 (MQ=255)                             aCGGTACGTTTCGGGTTTCTAACCATACCAGTACTTACAGTTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAg                                                                                                 <  1:230471/133‑1 (MQ=255)                                   cGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCAt                                                                                           >  1:61277/1‑133 (MQ=255)                                      ttCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGTTATCCGCAGTAATCAAGTTAGCTGCATAAg                                                                                        >  1:201208/1‑133 (MQ=255)                                          ggTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGc                                                                                    <  2:115013/133‑1 (MQ=255)                                                     aTACCAGTACTTACAGCTGCGGAATCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGCATTCCAGTTAGCTGCATAAGTTGCCGCATCACAcc                                                                         >  1:456972/1‑133 (MQ=255)                                                          aGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATgc                                                                    <  2:124304/133‑1 (MQ=255)                                                             aCTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATgccag                                                                 <  1:269631/133‑1 (MQ=255)                                                               ttACAGCTGCAGATTGAACACCCACGAAAGCCGACAGGCAGTGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATgccagcc                                                               <  2:194654/133‑1 (MQ=255)                                                                 aCAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCcaa                                                             >  2:424515/1‑133 (MQ=255)                                                                 aCAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCcaa                                                             >  2:369290/1‑133 (MQ=255)                                                                 aCAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCcaa                                                             >  2:11387/1‑133 (MQ=255)                                                                 aCAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGAATACGTTACCGCATCAAACCAATGCCAGCcaa                                                             >  2:190663/1‑133 (MQ=255)                                                                     cTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAAt                                                         >  2:361778/1‑133 (MQ=255)                                                                      tGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATa                                                        >  1:109894/1‑133 (MQ=255)                                                                        cGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTAGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGc                                                      >  1:496514/1‑133 (MQ=255)                                                                              cAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTAACCGCAGTATTCCAGATAACTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGtcat                                                <  2:510176/133‑1 (MQ=255)                                                                                  acCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATcacc                                            <  1:224892/133‑1 (MQ=255)                                                                                              ccACAGGCAGAGCAGACTACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGa                                >  1:505102/1‑133 (MQ=255)                                                                                              ccACAGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGa                                >  1:344118/1‑133 (MQ=255)                                                                                                acaGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAAc                              <  2:109894/133‑1 (MQ=255)                                                                                                  aGGCAGAGCAGAATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCa                            >  1:455590/1‑133 (MQ=255)                                                                                                      agagcagaTTACTTTTAATGATCGCATGATAATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCAc                        <  1:190663/133‑1 (MQ=255)                                                                                                      agagcagaATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCAc                        <  2:348513/133‑1 (MQ=255)                                                                                                      agagcagaATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCAc                        <  1:369290/133‑1 (MQ=255)                                                                                                      agagcagaATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCAc                        <  1:406882/133‑1 (MQ=255)                                                                                                            gaATACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGAcc                  <  1:361778/133‑1 (MQ=255)                                                                                                               tACTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAAt               >  2:41764/1‑133 (MQ=255)                                                                                                                 cTTTTAATGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAg             <  1:424515/133‑1 (MQ=255)                                                                                                                       aTGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGATTAAGAACCAGCGCCAGACCAAGAGTGGTTa       >  1:194919/1‑133 (MQ=255)                                                                                                                       aTGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAGTAAGAACAAGCGCCGGACCAATAGTGGTTa       >  1:139270/1‑133 (MQ=255)                                                                                                                       aTGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCCGACCAAAAGTGGTTa       >  1:226989/1‑133 (MQ=255)                                                                                                                       aTGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTa       >  2:316674/1‑133 (MQ=255)                                                                                                                        tGATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTaa      <  2:455590/133‑1 (MQ=255)                                                                                                                         gATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTaaa     >  2:347411/1‑133 (MQ=255)                                                                                                                         gATCGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTaaa     <  1:494171/133‑1 (MQ=255)                                                                                                                            cGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACgg  <  2:27372/133‑1 (MQ=255)                                                                                                                            cGCATGATCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACgg  >  1:34083/1‑133 (MQ=255)                                                                                                                                   |                                                                                                                              ACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGG  >  NC_000913/4359345‑4359600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑