Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	310,333	G→A	G113G (GGC→GGT)	ecpA ←	common pilus major subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	310,333	0	G	A	100.0%	114.0 / NA	34	G113G (GGC→GGT)	ecpA	common pilus major subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/22);  total (12/22)

TCCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGCCACGTCAAAT  >  NC_000913/310214‑310462                                                                                                                        |                                                                                                                                  tCCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATTCa                                                                                                                      <  2:330576/133‑1 (MQ=255) tCCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCa                                                                                                                      <  2:86674/133‑1 (MQ=255) tCCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCa                                                                                                                      >  2:378466/1‑133 (MQ=255) tCCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCa                                                                                                                      >  1:3269/1‑133 (MQ=255) tCCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGGTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGGATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCa                                                                                                                      >  2:355940/1‑133 (MQ=255)  ccTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCAc                                                                                                                     >  1:483346/1‑133 (MQ=255)     gtgCGGTGGTACGATCGCTGGCATCGTACCCGCTAGCCAGCCGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGcc                                                                                                                  <  2:150844/133‑1 (MQ=255)           tggtACGATTGCTGGGATTGTAGCCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACAtt                                                                                                            <  1:223594/133‑1 (MQ=255)           tggtACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACAtt                                                                                                            >  2:114058/1‑133 (MQ=255)           tggtACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGCATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACAtt                                                                                                            >  1:86506/1‑133 (MQ=255)            ggtACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTc                                                                                                           >  1:305239/1‑133 (MQ=255)            ggtACGATTGCGGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTTCCGCCCAGGACGCCGTTGGCGGTATCGATCATCACGGTATCGCCACTTTTTTCGCCTGACGCACCGTTATATTCCACGCCCACATTc                                                                                                           >  2:218000/1‑133 (MQ=255)             gtACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTCa                                                                                                          >  1:358136/1‑133 (MQ=255)                  aTTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCTCACATTCAGtgtg                                                                                                     <  2:289117/133‑1 (MQ=255)                       tGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTCAGGGTGGAAcc                                                                                                >  1:198664/1‑133 (MQ=255)                                 aCCGTTAGCCCGCGGCCTAAGGTTCCCGCCCAGTACGCCGTCGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATcc                                                                                      <  2:198664/133‑1 (MQ=255)                                   cGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCaa                                                                                    <  2:385070/133‑1 (MQ=255)                                    gTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGCTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAAc                                                                                   >  2:52207/1‑133 (MQ=255)                                     ttAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACt                                                                                  <  1:333945/133‑1 (MQ=255)                                       aGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAAACCACGCCCACATGCCGCGTGGAACCGGAGGGATCCAACTgg                                                                                >  2:248825/1‑133 (MQ=255)                                        gCCAGCGGCCTAAGGTTGTCGCCCAGTACGTCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTggg                                                                               <  1:197881/133‑1 (MQ=255)                                         ccAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGt                                                                              <  2:305239/133‑1 (MQ=255)                                           aGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTa                                                                            <  1:378466/133‑1 (MQ=255)                                                gCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAAtgtg                                                                       <  2:309071/133‑1 (MQ=255)                                                 cTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATgtgt                                                                      <  1:355940/133‑1 (MQ=255)                                                     ggTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGt                                                                  <  2:3269/133‑1 (MQ=255)                                                        tGCCGCCCAGTACGCCGTTGGCGGTATCGATAATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGAt                                                               <  2:39234/133‑1 (MQ=255)                                                          ccgccCAGCACGCCGATGACGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATaa                                                             <  1:239313/133‑1 (MQ=255)                                                                    cGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGt                                                   <  2:5029/133‑1 (MQ=255)                                                                    cGCCGTTGCCGGTATGGATCATGACGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGt                                                   <  1:189578/133‑1 (MQ=255)                                                                                   cGATCATCACGGTATTGCTAGTCTTTTCGAGTGCCGCACCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGt                                    <  1:248825/133‑1 (MQ=255)                                                                                            cGGTATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGa                           >  1:41730/1‑133 (MQ=255)                                                                                               tATCGCCAGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGAGAGGTCAAATTAAAGGAGGTACGCGTTGAGTc                        >  2:245478/1‑133 (MQ=255)                                                                                                aTCGGCAGCTTTTTCAACAGCCGCACCGTTATAATCCAAGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCa                       <  1:218000/133‑1 (MQ=255)                                                                                                      aGTTTTTTCGACTGCCGCACCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCg                 <  2:358136/133‑1 (MQ=255)                                                                                                            ttCGACTGCCGCACCATTATAATCCATGACCAGATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGcc           <  2:238587/133‑1 (MQ=255)                                                                                                               gACTGCCGCACCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGCCACg        <  2:289559/133‑1 (MQ=255)                                                                                                                     cgcaCCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGCCACGTCAAAt  <  2:48743/133‑1 (MQ=255)                                                                                                                        |                                                                                                                                  TCCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGCCACGTCAAAT  >  NC_000913/310214‑310462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑