Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,586,296	G→A	Q156* (CAG→TAG)	hsdR ←	type I restriction enzyme EcoKI endonuclease component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,586,296	0	G	A	100.0%	73.0 / NA	22	Q156* (CAG→TAG)	hsdR	type I restriction enzyme EcoKI endonuclease component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/10);  total (12/10)

CGCTTCGGTTTCCTGCTGTTTGCCTTCCAGAATGGCGATATAGCCGTTCAGGGCAACCAGCTTCTGCTGTTGCGCTTCGACTTCTGCCTGAGTCTGCGCTTTTTCTCGCACCTGCTGTTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAACACCGGCACCGGGAAGTCATAATCTTTAGTGACCAGACGGTAGTACCAGA  >  NC_000913/4586180‑4586415                                                                                                                     |                                                                                                                        cgcTTCGGTTTCCTGCTGTTTGCCTTCCAGAATGGCGATATAGCCGTTCAGGGCAACCAGCTTCTGCTGTTGCGCTTCGACTTCTGCCTGAGTCTGCGCTTTTTCTCGCACCTGCTATTCAAGCTGTTGtttt                                                                                                         >  1:416284/1‑133 (MQ=255) cgcTTCGGTTTCCTGCTGTTTGCCTTCCAGAATGGCGATATAGCCGTTCAGGGCAACCAGCTTCTGCTGTTGCGCTTCGACTTCTGCCTGAGTCTGCGCTTTTTCTCGCACCTGCTATTCAAGCTGTTGtttt                                                                                                         >  1:450370/1‑133 (MQ=255)                ctgTTTGCCTTCCAGAATGGCGATATAGCCGTTCAGGGCAACCAGCTTCTGCTGTTGCGCTTCGACTTCTGCCTGAGTCTGCGCTTTTTCTCGCACCTGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTcc                                                                                          <  2:450370/133‑1 (MQ=255)                           ccAGAATGGCGATATAGCCGTTCAGGGCAACCAGCTTCTGCTGTTGCGCTTCGACTTCTGCCTGAGTCTGCGCTTTTTCTCGCACCTGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATagag                                                                               <  2:416284/133‑1 (MQ=255)                                    cGATATAGCCGTTCAGGGCAACCAGCTTCTGCTGTTGCGCTTCGACTTCTGCCTGAGTCTGCGCTTTTTCTCGCACCTGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTcacc                                                                      <  2:85061/133‑1 (MQ=255)                                      atatAGCCGTTCAGGGCAACCAGCTTCTGCTGTTGCGCTTCGACTTCTGCCTGAGTCTGCGCTTTTTCTCGCACCTGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTcaccac                                                                    >  2:109042/1‑133 (MQ=255)                                              gTTCAGGGCAACCAGCTTCTGCTGTTGCGCTTCGACTTCTGCCTGAGTCTGCGCTTTTTCTCGCACCTGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGc                                                            <  1:109042/133‑1 (MQ=255)                                                    ggCAACCAGCTTCTGCTGTTGCGCTTCGACTTCTGCCTGAGTCTGCGCTTTTTTTCGCACCTGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGAGATAGCGCCTTTCACCTCGTTTAGGCAAcaca                                                      >  2:251581/1‑133 (MQ=255)                                                             cTTCTGCTGTTGCGCTTCCACTGCTGCCTGGGTCTGCGCTTTTTTTCGCACCTGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAAcaccggc                                             <  1:251581/133‑1 (MQ=255)                                                                     gTTGCGCTTCGACTTCTGCCTGAGTCTGCGCTTTTTCTCGCACCTGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAACACCGGCACCggg                                       <  2:113456/131‑1 (MQ=255)                                                                     gTTGCGCTTCGACTTCTGCCTGAGTCTGCGCTTTTTCTCGCACCTGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAACACCGGCACCggg                                       >  1:113456/1‑131 (MQ=255)                                                                      ttGCGCTTCGACTTCTGCCTGAGTCTGCGCTTTTTCTCGCACCTGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAACACCGGCACCGGGAAg                                    >  2:273775/1‑133 (MQ=255)                                                                        gcgcTTCGACTTCTGCCTGAGTCTGCGCTTTTTCTCGCACCTGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAGCACCGGCACCGGGAAGTc                                  >  1:196319/1‑133 (MQ=255)                                                                        gcgcTTCGACTTCTGCCTGAGTCTGCGCTTTTTCTCGCACCTGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAACACCGGCACCGGGAAGTc                                  >  1:318093/1‑133 (MQ=255)                                                                             tCGACTTCTGCCTGAGTCTGCGCTTTTTCTCGCACCTGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGACAAAACAAACACCGGCACCGGGAAGTCATAAt                             >  2:156539/1‑133 (MQ=255)                                                                                              cTGCGCTTTTTCTCGCACCTGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAACACCGGCACCGGGAAGTCATAATCTTTAGTGACCAGACgg            <  1:351936/133‑1 (MQ=255)                                                                                              cTGCGCTTTTTCTCGCACCTGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAACACCGGCACCGGGAAGTCATAATCTTTAGTGACCAGACgg            <  2:294161/133‑1 (MQ=255)                                                                                                 cgcTTTTTCTCGCACCTGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAACACCGGCACCGGGAAGTCATAATCTTTAGTGACCAGACGgtag         >  2:456913/1‑133 (MQ=255)                                                                                                 cgcTTTTTCTCGCACCCGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAACGCCGGCACCTGGAGGTCATAATCTTTTGTGAGCAGACGgtag         >  2:130912/1‑133 (MQ=255)                                                                                                       ttCTCGCACCTGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAACACCGGCACCGGGAAGACATGATCGTTAGTGACAAGACGGTAGTACCAg   >  2:144594/1‑133 (MQ=255)                                                                                                        tctcGCACCTGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAACACCGGCACCGGGAAGTCATAATCTTTAGTGACCAGACGGTAGTACCAGa  <  1:349113/133‑1 (MQ=255)                                                                                                        tctcGCACCTGCTATTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAACACCGGCACCGGGAAGTCATAATCTTTAGTGACCAGACGGTAGTACCAGa  <  1:13323/133‑1 (MQ=255)                                                                                                                     |                                                                                                                        CGCTTCGGTTTCCTGCTGTTTGCCTTCCAGAATGGCGATATAGCCGTTCAGGGCAACCAGCTTCTGCTGTTGCGCTTCGACTTCTGCCTGAGTCTGCGCTTTTTCTCGCACCTGCTGTTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAACACCGGCACCGGGAAGTCATAATCTTTAGTGACCAGACGGTAGTACCAGA  >  NC_000913/4586180‑4586415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑