Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,593,136	T→C	S494P (TCT→CCT)	tsr →	methyl‑accepting chemotaxis protein Tsr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,593,136	0	T	C	100.0%	51.1 / NA	16	S494P (TCT→CCT)	tsr	methyl‑accepting chemotaxis protein Tsr
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/6);  total (10/6)

TTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAGCGGTTGCTGAGATGGACCGGGTAACTCAACAGAACGCCGCGCTGGTGGAAGAGTCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGC  >  NC_000913/4593020‑4593262                                                                                                                     |                                                                                                                               ttATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAGCGGTTGCTGAGATGGACCGGGTAACTCAACAGAACGCCGCGCTGGTGGAAGAGCCTGCCGCTgccgccgc                                                                                                                <  1:475568/133‑1 (MQ=255)        cGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAGCGGTTGCTGAGCTGGACCGGGTAACTCAACAGAACGCCGCGCTGGTGGAAGAGCCTGCCGCTGCCGCCGCCGCGCTg                                                                                                         >  1:495901/1‑133 (MQ=255)                     gcttctgATGAGCAGAGCGGTGGTATCGATCAGGTTGGCTTAGCGGTTGCTGAGATGGACCGGGTAACTCAACAGAACGCCGCGCTGGTGGAAGAGCCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCa                                                                                            <  1:427504/133‑1 (MQ=255)                                    agCCGTGGTATCGATCAGGTTGGCTTAGCGGTTGCTGAGATGGACCGGGTAACTCAACAGAACGCCGCGCTGGTGGAAGAGCCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAg                                                                             >  2:466924/1‑133 (MQ=255)                                        gTGGTATCAATCAGGATGCCTTAGCGGTTGCTGAGATGGACCGGGTAACTCAACAGAACGCCGCGCTGGTGGAAGAGCCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCAGACCGAAgcagt                                                                         <  1:149486/133‑1 (MQ=255)                                           gTATCGATCAGGTTGGCTTAGCGGTTGCTGAGATGGACCGGGTAACTCAACAGAACGCCGCGCTGGTGGAAGAGCCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAgcagtggc                                                                      >  2:397603/1‑133 (MQ=255)                                           gTATCGATCAGGTTGGCTTAGCGGTTGCTGAGATGGACCGGGTAACTCAACAGAACGCCGCGCTGGTGGAAGAGCCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCCGACCGAAgcagtggc                                                                      >  1:204075/1‑133 (MQ=255)                                                 aTCAGGTTGGCTTAGCGGTTGCTGAGATGGACCGGGTAACTCAACAGAACGCCGCGCTGGTGGAAGAGCCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGtt                                                                <  2:27898/133‑1 (MQ=255)                                                      gTTGGCTTAGCGGTTGCTGAGATGGACCGGGTAACTCAACAGAACGCCGCGCTGGTGGAAGAGCCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGa                                                           >  2:12940/1‑133 (MQ=255)                                                                    tGCTGAGATGGACCGGGTAACTCAACAGAACGCCGCGCTGGTGGAAGAGCCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAAcagcag                                             >  2:490466/1‑133 (MQ=255)                                                                                   ggTAACTCAAAAGAATGCCGCGCTAGTGTAAGCGCCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCt                              <  2:167191/133‑1 (MQ=255)                                                                                     tAACTCAACAGAACGCCGCGCTGGTGGAAGAGCCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAGCAGTGGCTGTGTTCCGGATTGAGCAACAGTAGCTTGAAACATCGGCtgt                            >  2:203888/1‑133 (MQ=255)                                                                                             cAGAACGCCGCGCTGGTGGAAGAGCCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTaaaaa                    >  2:359240/1‑133 (MQ=255)                                                                                                     cgcgCTGGTGGAAGAGCCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGGAACAGCAGGGTGGAACCTCGGCTGGTGTAACAACCGTGACg            >  2:206179/1‑133 (MQ=255)                                                                                                          tggtggAAGAGCCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAACAGCGTGAAACATCGGGTGTGGTAAGAACCGTGACGCCAGc       >  2:163246/1‑133 (MQ=255)                                                                                                               gAAGAGCCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTgcgc  <  1:408960/133‑1 (MQ=255)                                                                                                                     |                                                                                                                               TTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAGCGGTTGCTGAGATGGACCGGGTAACTCAACAGAACGCCGCGCTGGTGGAAGAGTCTGCCGCTGCCGCCGCCGCGCTGGAAGAGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGC  >  NC_000913/4593020‑4593262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑