Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,632,628	A→G	K632K (AAA→AAG)	slt →	soluble lytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,632,628	0	A	G	100.0%	113.5 / NA	33	K632K (AAA→AAG)	slt	soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/18);  total (15/18)

AGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGC  >  NC_000913/4632509‑4632751                                                                                                                        |                                                                                                                            aGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTtgatga                                                                                                                >  1:125997/1‑133 (MQ=255)             aTCGACGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAAt                                                                                                    >  1:204568/1‑133 (MQ=255)                gACGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATggg                                                                                                 <  2:306673/133‑1 (MQ=255)                  cGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGa                                                                                               <  2:480242/133‑1 (MQ=255)                  cGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGa                                                                                               <  1:8775/133‑1 (MQ=255)                   gCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGAc                                                                                              <  1:394747/133‑1 (MQ=255)                     aGTGGCATTTGTCGATAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGAcgt                                                                                            <  2:310706/133‑1 (MQ=255)                     aGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGAcgt                                                                                            >  2:434081/1‑133 (MQ=255)                                  gagaGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCg                                                                               <  2:125997/133‑1 (MQ=255)                                  gagaGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCg                                                                               <  1:49317/133‑1 (MQ=255)                                          tCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTa                                                                       <  1:28821/133‑1 (MQ=255)                                           ccATTCTCCGAGACGGGCTGTTCTGTGAAGGACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTAt                                                                      <  1:139851/133‑1 (MQ=255)                                            cATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATg                                                                     >  1:4189/1‑133 (MQ=255)                                              ttCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGGTACAGAGCCGCACTTTTATGat                                                                   >  1:173543/1‑133 (MQ=255)                                               tctcCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGata                                                                  <  2:505922/133‑1 (MQ=255)                                               tctcCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGata                                                                  <  2:271802/133‑1 (MQ=255)                                                   cGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCt                                                              >  2:501695/1‑133 (MQ=255)                                                        cgcgcgGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGt                                                         <  2:204568/133‑1 (MQ=255)                                                        cgcgcgGTTACGTGATGAACGTGCTGGCGTATCACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGt                                                         <  2:166872/133‑1 (MQ=255)                                                           gcgGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTCATCCGCACGTTCATCATATGCCATCGTAct                                                      >  1:157492/1‑133 (MQ=255)                                                                      aagaaCGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGt                                           <  2:56945/133‑1 (MQ=255)                                                                              gCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCgg                                   >  2:186176/1‑133 (MQ=255)                                                                                  gCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGATATAGAACTCTTTAGCTAGTAACACCggggta                               >  2:235269/1‑131 (MQ=255)                                                                                          cGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCAtttt                       >  1:403847/1‑133 (MQ=255)                                                                                            cTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACACCCGGGGGAGGCATTTTGc                     >  1:200482/1‑133 (MQ=255)                                                                                            cTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTATAGCGAGGACAGCCGGGGGAGCCATTTTGc                     >  1:164910/1‑133 (MQ=255)                                                                                            cTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCAATCGTACTATTTAGCGCGTACAACCGGGGGAGGCATTTTGc                     >  2:144835/1‑133 (MQ=255)                                                                                                         tttCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAAc        >  2:483271/1‑133 (MQ=255)                                                                                                             aTGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATg    <  1:63110/133‑1 (MQ=255)                                                                                                              tGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATgg   <  1:186176/133‑1 (MQ=255)                                                                                                              tGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATgg   >  2:113278/1‑133 (MQ=255)                                                                                                              tGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATgg   <  1:483271/133‑1 (MQ=255)                                                                                                               gggggATAGGCCGACTTTAATGTGCGCCACAGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGc  <  1:235269/133‑1 (MQ=255)                                                                                                                        |                                                                                                                            AGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGC  >  NC_000913/4632509‑4632751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑