Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 319,208 C→T G265D (GGT→GAT)  rclA ← putative pyridine nucleotide‑disulfide oxidoreductase RclA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913319,2080CT94.2% 157.7 / ‑0.6 52G265D (GGT→GAT) rclAputative pyridine nucleotide‑disulfide oxidoreductase RclA
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (27/22);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTTCATGGTGACTGATTCGCTC  >  NC_000913/319079‑319335
                                                                                                                                 |                                                                                                                               
cGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCgg                                                                                                                              >  2:514726/1‑133 (MQ=255)
cGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGCTTACTGCGATATCgg                                                                                                                              >  1:187969/1‑133 (MQ=255)
        atcCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCt                                                                                                                      >  1:342277/1‑133 (MQ=255)
        acccAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCt                                                                                                                      <  2:333636/131‑1 (MQ=255)
             gTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATg                                                                                                                 <  2:4447/133‑1 (MQ=255)
                  atGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACg                                                                                                            <  1:278869/133‑1 (MQ=255)
                    gtaagtaaATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGaa                                                                                                          <  2:86555/133‑1 (MQ=255)
                       agtaaATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCg                                                                                                       >  1:269511/1‑133 (MQ=255)
                        gtaaATTGCAGCCCGCCGGTAACATCTCCCATCGCCCACATATTTTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGATCGTTTACTGCGATATCGGCATTTTCTGGATGTAAGGAAGCgg                                                                                                      >  2:382680/1‑133 (MQ=255)
                        gtaaATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTGGTTTACTGAGATCTCGTCATTTTCTGGATGTAACGAAGCgg                                                                                                      >  2:194750/1‑133 (MQ=255)
                        gtaaATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCgg                                                                                                      >  2:394741/1‑133 (MQ=255)
                        gtaaATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCgg                                                                                                      >  1:256567/1‑133 (MQ=255)
                        gtaaATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCgg                                                                                                      <  2:350305/133‑1 (MQ=255)
                        gtaaATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCgg                                                                                                      >  1:447737/1‑133 (MQ=255)
                           aaTTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAg                                                                                                   >  1:129335/1‑133 (MQ=255)
                            aTTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACg                                                                                                            >  2:67458/1‑123 (MQ=255)
                            aTTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACg                                                                                                            <  1:67458/123‑1 (MQ=255)
                            aTTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGc                                                                                                  <  2:269511/133‑1 (MQ=255)
                                   cccGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTga                                                                                           >  2:57861/1‑133 (MQ=255)
                                          gTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCgg                                                                                    <  2:256567/133‑1 (MQ=255)
                                          gTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCgg                                                                                    <  2:447737/133‑1 (MQ=255)
                                          gTAACATCGCCCATCTCCCAAATATTGTCTACGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCgg                                                                                    <  1:184756/133‑1 (MQ=255)
                                          gTAACATCCCCCATCCCCCAAACATTGTCTCCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCgg                                                                                    <  1:154070/133‑1 (MQ=255)
                                                  tcCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTAtt                                                                            >  1:125207/1‑133 (MQ=255)
                                                  tcCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTAtt                                                                            <  2:333588/133‑1 (MQ=255)
                                                  tcCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTAtt                                                                            <  2:342277/133‑1 (MQ=255)
                                                      aTCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATATAACGAAGCGGTGGACTATTGACGAACGGAAACTATTaaaa                                                                        >  2:245872/1‑131 (MQ=255)
                                                         gCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTg                                                                     <  2:482887/133‑1 (MQ=255)
                                                                     tCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCa                                                         >  1:319442/1‑133 (MQ=255)
                                                                        gCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGtt                                                      >  2:509696/1‑133 (MQ=255)
                                                                         cGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTg                                                     >  2:29722/1‑133 (MQ=255)
                                                                              gtATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGt                                                >  1:260794/1‑133 (MQ=255)
                                                                                 tGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCt                                             <  1:258032/133‑1 (MQ=255)
                                                                                 tGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCt                                             <  1:262616/133‑1 (MQ=255)
                                                                                   tAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCg                                           >  1:83515/1‑133 (MQ=255)
                                                                                   tAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCg                                           <  1:509696/133‑1 (MQ=255)
                                                                                      tCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTa                                        >  1:86108/1‑133 (MQ=255)
                                                                                        gCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGTATCCACCGCCAATTAGGCGTGGTCGCTAtg                                      >  1:169402/1‑133 (MQ=255)
                                                                                        gCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTAtg                                      >  1:307465/1‑133 (MQ=255)
                                                                                        gCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCCATTAACAGGGCAGCCAACGCCAGTTAGGCGTGCTCGCTAtg                                      >  2:180003/1‑133 (MQ=255)
                                                                                         cTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTAtgc                                     >  1:346873/1‑133 (MQ=255)
                                                                                         cTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTAtgc                                     >  1:411581/1‑133 (MQ=255)
                                                                                               aaCGACAATTGCCCTGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTAtgcacttgc                               >  1:69797/1‑133 (MQ=255)
                                                                                               aaCGACAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGTATCCACCGCCAGTTGGGCGTGCTCGCTAtgcacttgc                               <  2:16807/133‑1 (MQ=255)
                                                                                                   aCAATTGCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTATgcacttgcactt                           <  1:48598/133‑1 (MQ=255)
                                                                                                         gCCCCGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGAtttt                     >  1:428794/1‑133 (MQ=255)
                                                                                                            ccGCGCTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAATCTATTATCAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTTCAt                  <  2:129335/133‑1 (MQ=255)
                                                                                                               cgcTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTTCATGGt               >  2:502889/1‑133 (MQ=255)
                                                                                                               cgcTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTTCATGGt               >  1:468114/1‑133 (MQ=255)
                                                                                                                 cTCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTGGGGCGTGCACGCAATGAACTTGCACGTGATGTTCATGGTGa             >  2:138437/1‑133 (MQ=255)
                                                                                                                  tCGTTTACTGCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTTCATGGTGAc            <  1:514726/133‑1 (MQ=255)
                                                                                                                       tagtGCGATGTCGGCCTTTTCTGGCTGTAACGCAGCGGTAGGCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTTCATGGTGACTGAtt       <  2:225248/130‑1 (MQ=255)
                                                                                                                       tACTGCGACATCGGCATTATCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTTCATGGTGACTGAtt       <  1:245872/133‑1 (MQ=255)
                                                                                                                           gCGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTTCATGGTGACTGATTCGCt   <  1:294015/133‑1 (MQ=255)
                                                                                                                            cGATATCGGCTTTTTTTGGATGTACCGAGGCGGTAGCCGGTGGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTTCATGGTGACTGATTCGCTc  <  2:187969/133‑1 (MQ=255)
                                                                                                                            cGATATCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTTCATGGTGACTGATTCGCTc  <  2:69797/133‑1 (MQ=255)
                                                                                                                                 |                                                                                                                               
CGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTTCATGGTGACTGATTCGCTC  >  NC_000913/319079‑319335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑