| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 16,753 | A→G | *51Q (TAA→CAA) *70Q (TAA→CAA) |
hokC ← mokC ← |
protein HokC regulatory protein MokC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 16,753 | 0 | A | G | 95.5% | 61.0 / ‑4.1 | 22 | *51Q (TAA→CAA) *70Q (TAA→CAA) | hokC mokC | protein HokC regulatory protein MokC |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (17/4); total (18/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCG > NC_000913/16625‑16877 | cTGACACCCAGCAAACCGCAATTATAGATCGCCAGCCTTCGTCTTAACGAGGCCCCGAGGGGTCGTATTCTTCAGATGGTTCACCCTTTAAGTTAGCGCTTATGGGATCCCTCCCCGCCGTTGCTCTTGCTCg < 2:158040/133‑1 (MQ=9) aGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTGCTCGGATTc > 1:460673/1‑133 (MQ=255) aGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTGCTCGGATTc > 1:4836/1‑133 (MQ=255) aGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTGCTCGGATTc > 1:78634/1‑133 (MQ=255) aGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGCATCACTCGCCGCCGTTGCTCTTGCTCGGATTc > 2:237056/1‑133 (MQ=255) ccTACAAACCAAAATAGAAAAACGAGAGGCATCGTCTTAACGAGGCACAGAGGCGCCGCTTTCTCCGGAGGGTTCAACCCTAAAGTGGCCGTTTAGGGGACGGCGCCCCGCCGTTGCTCTTGCTCGGATTCGt < 2:342697/133‑1 (MQ=9) ccTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTACTCGGATTCGt > 1:296688/1‑133 (MQ=255) aCAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTGCTCGGATTCGTAAg > 2:499457/1‑133 (MQ=255) aaTTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTGCTCGGATTCGTAAGCCGTGaaaa > 2:412855/1‑133 (MQ=255) ttGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTGCTCGGATTCGTAAGCCGTGAAAACa > 1:289378/1‑133 (MQ=255) tGAAGAAGGCGAGCCATGGTCTCAACGGGGCCCCGAGGCGCCGCATTCTTCAGATGTTCCACCCCTTAATTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTGCTCGGATTCGTAAGCCGTGAAAACAg < 2:460673/133‑1 (MQ=255) ggCACCGGGGCGTCGCATTCTTCAGATGTTTCACCCCTAAAGTTAGCGCTTATGGCATCACCCCCCGCCGTTGCTCTTGCTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGAtgtg < 2:78634/133‑1 (MQ=37) cGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTGGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTGCTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAAGCt > 2:299568/1‑133 (MQ=255) aGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTGCTCGGATTCGTAAGCCGTGCAAACAGCAACCTTCGTATGGCCAGTACGGAAGTGAACCTca > 2:190827/1‑133 (MQ=37) tCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTGCTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAg > 1:509709/1‑133 (MQ=255) tCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTAGCTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGACTTTTCTCGTTACCAg > 2:333111/1‑133 (MQ=255) aaGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTGCTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCAc > 1:122034/1‑133 (MQ=255) aGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTGCTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAAACTCACAGAGGACGTTTATCGTTACCAGCGCAGCCACTAcgg > 2:189959/1‑133 (MQ=255) aTGGGATCACTCCCCGCCGTTGCTCTTGCTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGAt > 1:472980/1‑133 (MQ=255) cACTCCCCGCCGTTGCTCTTGCTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATg > 1:414540/1‑133 (MQ=255) tCCCCGCCGTTGCTCTTGCTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTATCGTTACCGGCGACGCGACTACGGCGGTGAAACAGATGACg > 2:170444/1‑133 (MQ=255) cccGCCGTTGCTCTTGCTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGAt > 1:311199/1‑133 (MQ=255) ttgctcttgctcGGAGTCTTAATCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTCCCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCg < 1:190827/133‑1 (MQ=25) | CTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCG > NC_000913/16625‑16877 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |