| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 350,390 | C→T | H127Y (CAT→TAT) | prpC → | 2‑methylcitrate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 350,390 | 0 | C | T | 100.0% | 40.6 / NA | 16 | H127Y (CAT→TAT) | prpC | 2‑methylcitrate synthase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (6/10); total (6/10) | |||||||||||
GCGCGGTTTACCGGCTAACGTGCGTACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCG > NC_000913/350269‑350519 | gcgcGGTTTACCGGCTAACGTGCGTACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGTATACCGTctct > 2:268795/1‑133 (MQ=255) tttACCGGCTAACGTGCGTACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGTATACCGTCTCTGgcgcg < 1:497430/133‑1 (MQ=255) tttACCGGCTAACGTGCGTACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGTATACCGTCTCTGgcgcg < 2:311646/133‑1 (MQ=255) tttACCGGCTAACGTGCGTACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGTATACCGTCTCTGgcgcg < 2:504200/133‑1 (MQ=255) cGTACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATTGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGTATACCGTCTCTGGCGCGCGGGATATTGCCGACa < 2:212730/133‑1 (MQ=255) tACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGTATACCGTCTCTGGCGCGCGGGATATTGCCGACaaa > 2:89915/1‑133 (MQ=255) tGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGTATACCGTGTCTAGCGGGCGGGATAATGCCGACAAActgct > 2:152047/1‑133 (MQ=255) tCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGTATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTcc < 1:32655/133‑1 (MQ=255) tCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGTATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTcc < 1:89915/133‑1 (MQ=255) aCCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGTATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTa < 1:441154/133‑1 (MQ=255) gatgTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGTATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATc < 1:225780/133‑1 (MQ=255) gcACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGTATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCca > 1:126134/1‑133 (MQ=255) cACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGTATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGATATCACTACAGCcac > 1:229798/1‑133 (MQ=255) tttCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGTATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGa < 1:268795/133‑1 (MQ=255) gcgcTCGGCTGCACGCTGCCAGAAAAAGAGGGGTATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCa > 2:50530/1‑133 (MQ=255) gggTATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATcg < 1:71659/133‑1 (MQ=255) | GCGCGGTTTACCGGCTAACGTGCGTACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCG > NC_000913/350269‑350519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |