Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	363,780	C→T	A842A (GCG→GCA)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	363,780	0	C	T	100.0%	84.3 / NA	25	A842A (GCG→GCA)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/13);  total (12/13)

GGCCAATCCGCGCCGGATGCGGTGTATCGCTCGCCACTTCAACATCAACGGTAATCGCCATTTGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCACGCGTGAGCGGTCGTAATCAGCACCGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGAC  >  NC_000913/363653‑363884                                                                                                                                |                                                                                                         ggCCAATCCGCGCCGGATGCGGTGTATCGCTCGCCACTTCAACATCAACGGTAATCGCCATTTGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTg                                                                                                     <  1:428433/133‑1 (MQ=255)   ccAATCCGCGCCGGATGCGGTGTATCGCTCGCCACTTCAACATCAACGGTAATCGCCATTTGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAg                                                                                                   <  1:124969/133‑1 (MQ=255)   ccAATCCGCGCCGGATGCGGTGTATCGCTCGCCACTTCAACATCAACGGTAATCGCCATTTGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAg                                                                                                   <  2:411610/133‑1 (MQ=255)   ccAATCCGCGCCGGATGCGGTGTATCGCCCGCAACTTCAACTTCAACGGTAATCGCCATTTGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAg                                                                                                   <  2:159172/133‑1 (MQ=255)       tCCGCGCCGGATGCGGTGTATCGCTCGCCACTTCAACATCAACGGTAATCGCCATTTGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGt                                                                                               >  2:23820/1‑133 (MQ=255)            gcCGGATGCGGTGTATCGCTCGCCACTTCAACATCAACGGTAATCGCCATTTGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGTCGTaa                                                                                          >  2:409272/1‑133 (MQ=255)              cGGATGCGGTGTATCGCTCGCCACTTCAACATCAACGGTAATCGCCATTTGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGTCGTAATc                                                                                        <  1:23820/133‑1 (MQ=255)                      gtgtATCGCTCGCCACTTCAACATCAACGGTAATCGCCATTTGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGTCGTAATCAGCACCGc                                                                                <  2:391674/133‑1 (MQ=255)                              ctcgcCACTTCAACATCAACGGTAATCGCCATTTGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGTCGTAATCAGCACCGCATCAGCaa                                                                        >  2:123773/1‑133 (MQ=255)                                 gcCACTTCAACATCAACGGTAATCGCCATTTGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGATCGGTCGTTATCGGCGCCGCATCAGCAAgtg                                                                     >  1:188323/1‑133 (MQ=255)                                 gcCACTTCAACATCAACGGTAATCGCCATTTGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGTCGTAATCAGCTCCGCATAAGCAAgtg                                                                     >  1:185039/1‑133 (MQ=255)                                   cACTTCAACATCAACGGTAATCGCCATTTGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGTCGTAATCAGCACCGCATCAGCAAGTGTa                                                                   >  1:482925/1‑133 (MQ=255)                                            aTCAACGGTAATCGCCATTTGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGTCGTAATCAGCACCGCATCAGCAAGTGTATCTGCCGTg                                                          <  2:482925/133‑1 (MQ=255)                                            aTCAACGGTAATCGCCATTTGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGTCGTAATCAGCACCGCATCAGCAAGTGTATCTGCCGTg                                                          <  1:426161/133‑1 (MQ=255)                                                aCGGTAATCGCCATTTGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGTCGTAATCAGCACCGCATCAGCAAGTGTATCTGCCGTGCACt                                                      >  2:370759/1‑133 (MQ=255)                                                              ttGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGTCGTAATCAGCACCGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACgctgct                                        >  2:389021/1‑133 (MQ=255)                                                               tGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGTCGTAATCAGCACCGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCtt                                       <  1:378588/133‑1 (MQ=255)                                                                   cACTCCCACCAATCCCGTAGGTCTTCCGGCAGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGTCGTAATCAGCACCGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGc                                   <  1:192992/133‑1 (MQ=255)                                                                    aCTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGTCGTAATCAGCACCGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGcc                                  <  1:123773/133‑1 (MQ=255)                                                                           tCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGTCGTAATCAGCACCGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAAt                           <  1:442082/133‑1 (MQ=255)                                                                                    tAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGTCGTAATCAGCACCGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCccgccg                  >  1:41448/1‑133 (MQ=255)                                                                                    tAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGTCGTAATCAGCACCGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCccgccg                  >  2:448174/1‑133 (MQ=255)                                                                                       gTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGTCGTAATCAGCACCGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCt               >  1:406288/1‑133 (MQ=255)                                                                                         tttCCGGCTGATTAATAAGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGTCGTAATCAGCACCGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTc             >  1:86634/1‑133 (MQ=255)                                                                                                    taaataaGGTTTTCCCCTGATGCTGCCATGCGTGAGCGGTCGTAATCAGCACCGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGAc  <  1:448174/133‑1 (MQ=255)                                                                                                                                |                                                                                                         GGCCAATCCGCGCCGGATGCGGTGTATCGCTCGCCACTTCAACATCAACGGTAATCGCCATTTGACCACTACCATCAATCCGGTAGGTTTTCCGGCTGATAAATAAGGTTTTCCCCTGATGCTGCCACGCGTGAGCGGTCGTAATCAGCACCGCATCAGCAAGTGTATCTGCCGTGCACTGCAACAACGCTGCTTCGGCCTGGTAATGGCCCGCCGCCTTCCAGCGTTCGAC  >  NC_000913/363653‑363884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑