Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	370,220	C→T	S537L (TCG→TTG)	mhpA →	putative 3‑(3‑hydroxyphenyl)propanoate/3‑hydroxycinnamate hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	370,220	0	C	T	100.0%	59.8 / NA	19	S537L (TCG→TTG)	mhpA	putative 3‑(3‑hydroxyphenyl)propanoate/3‑hydroxycinnamate hydroxylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/9);  total (10/9)

GTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGCTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCGCAAGAG  >  NC_000913/370100‑370342                                                                                                                         |                                                                                                                           gTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGCTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTTGGTGATGACGCt                                                                                                                <  2:42417/133‑1 (MQ=255)         cGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGCTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTTGGTGATGACGCTGACCCGcc                                                                                                        >  1:264578/1‑133 (MQ=255)            aGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGCTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTTGGTGATGACGCTGACCCGCCCTg                                                                                                     >  2:452618/1‑133 (MQ=255)                ggTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGCTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTTGGTGATGACGCTGACCCGCCCTGATGc                                                                                                 >  2:510555/1‑133 (MQ=255)                              aaTGCTTCGCTGGTGGTGATGCGCCCGGATCGCTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTTGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCg                                                                                   <  1:52188/133‑1 (MQ=255)                                tGCTTCGCTGGTGGTGATGCGCCCGGATCGCTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTTGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGaa                                                                                 >  2:126553/1‑133 (MQ=255)                                   ttCGCTGGTGGTGATGCGCCCGGATCGCTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTTGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAg                                                                              <  1:126553/133‑1 (MQ=255)                                     cGCTGGTGGTGATGCGCCCGGATCGCTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTTGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGt                                                                            >  2:367239/1‑133 (MQ=255)                                            ggtgATGCGCCCGGATCGCTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTTGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGa                                                                     >  2:394370/1‑133 (MQ=255)                                                  gcgcCCGGATCGCTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTTGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCa                                                               <  1:324940/133‑1 (MQ=255)                                                    gcCCGGATCGCTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTTGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACg                                                             <  2:496717/133‑1 (MQ=255)                                                    gcCCGGATCGCTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTTGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACg                                                             <  1:32013/133‑1 (MQ=255)                                                                             cgccaTTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTTGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTc                                    >  2:46434/1‑133 (MQ=255)                                                                                       cAAACCCTGGGCAAGACCCTGAATAAACTGGCGTTGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCtggtg                          >  1:366828/1‑133 (MQ=255)                                                                                                    aGACCCTGAATAAACTGGCGTTGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGAcc             <  2:366828/133‑1 (MQ=255)                                                                                                     gACCCTGAATAAACTGGCGTTGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTTCCACTCGTCGCCGGGGGGGTAAGTCGAccc            >  1:160886/1‑133 (MQ=255)                                                                                                     gACCCTGAATAAACTGGCGTTGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGAccc            <  2:460041/133‑1 (MQ=255)                                                                                                            aaTAAACTGGCGTTGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCGCaa     >  2:382981/1‑133 (MQ=255)                                                                                                             aTAAACTGGCGTTGGTGATGACTCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCGCAag    <  1:235592/133‑1 (MQ=255)                                                                                                               aaaCTGGCATTGGTGTTGTCGCCGACCCGCTCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCGCAagag  <  1:219015/133‑1 (MQ=255)                                                                                                                         |                                                                                                                           GTCGCCTGCGTAGCTGGTTCGCGCAACACAATGCTTCGCTGGTGGTGATGCGCCCGGATCGCTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGATATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCGCAAGAG  >  NC_000913/370100‑370342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑