Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	372,855	C→T	A247A (GCC→GCT)	mhpD →	2‑hydroxypentadienoate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	372,855	0	C	T	100.0%	77.4 / NA	24	A247A (GCC→GCT)	mhpD	2‑hydroxypentadienoate hydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/12);  total (12/12)

CGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACAT  >  NC_000913/372725‑372961                                                                                                                                   |                                                                                                           cGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGGCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCTCa                                                                                                          >  2:142392/1‑133 (MQ=255)     tAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCTCATAtt                                                                                                      >  1:30673/1‑133 (MQ=255)           ggCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCTCATATTGAAGGc                                                                                                <  1:262824/133‑1 (MQ=255)            gCCGTCTGGCTGGCATGCAAAATGTCCAATCTCGGTGAACCTCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCTCATATTGAAGGCa                                                                                               <  2:213901/133‑1 (MQ=255)            gCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCTCATATTGAAGGCa                                                                                               >  2:434759/1‑133 (MQ=255)             ccGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTCTTGGAGGTCATATAGAAGGCAt                                                                                              >  1:213901/1‑133 (MQ=255)                tCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCTCATATTGAAGGCATAgg                                                                                           >  1:425358/1‑133 (MQ=255)                  tggctggcACGCAAAATGGCCAGTCTGGGTGAACCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATTGTGGCGGTGAATGCGGGCGGTCGTTTTGAAGCTCATATTGGAGGTATAgggt                                                                                         >  2:38774/1‑131 (MQ=255)                                        gTCTGGGTGAACCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCTCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAg                                                                   >  2:36449/1‑133 (MQ=255)                                            gggTGAACCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATTGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCTCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCa                                                               <  1:38774/133‑1 (MQ=255)                                             ggTGAACCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCTCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAg                                                              <  2:30673/133‑1 (MQ=255)                                                              cGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCTCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTg                                             <  2:70070/133‑1 (MQ=255)                                                              cGGAGATATCATTCTTACCGCGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCTCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTg                                             >  2:391494/1‑133 (MQ=255)                                                                        aTTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCTCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTaa                                   >  2:374930/1‑133 (MQ=255)                                                                           cTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCTCATATTGAAGGCATAGGTTCAGTTGCTGCGACATGTTCAAGCGAAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCg                                >  2:203530/1‑133 (MQ=255)                                                                                 ggggCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCTCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGt                          >  1:425448/1‑133 (MQ=255)                                                                                    gCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCTCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGc                       >  2:320887/1‑133 (MQ=255)                                                                                              cGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCTCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGt             <  1:434759/133‑1 (MQ=255)                                                                                              cGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCTCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGt             <  2:425358/133‑1 (MQ=255)                                                                                                    tggCGGTGCATGCGGACGTTCGCTTTGAAGGTCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGc       <  1:142392/133‑1 (MQ=255)                                                                                                    tggCGGTGAATGCGGGCGATCGTTTTGAAGCTCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGc       <  1:374930/133‑1 (MQ=255)                                                                                                     ggCGGTGAATGCGGGCGATCGTTTTGAAGCTCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCa      >  1:60596/1‑133 (MQ=255)                                                                                                      gCGGTGAATGGGGGCGATCGTTTTGATGATCATATTGAAGGGATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCaa     <  2:230482/133‑1 (MQ=255)                                                                                                         gTGAATGCGGGCGATCGTTTTGAAGCTCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACAt  <  1:320887/133‑1 (MQ=255)                                                                                                         gTGAATGCGGGCGATCGTTTTGAAGCTCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACAt  <  2:114932/133‑1 (MQ=255)                                                                                                                                   |                                                                                                           CGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACAT  >  NC_000913/372725‑372961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑