| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 378,772 | A→G | V267A (GTC→GCC) | frmA ← | S‑(hydroxymethyl)glutathione dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 378,772 | 0 | A | G | 100.0% | 55.6 / NA | 17 | V267A (GTC→GCC) | frmA | S‑(hydroxymethyl)glutathione dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (9/8); total (9/8) | |||||||||||
TTCCATACGCGACCAGTGACCAACTGGAATGGACGGGTGGAGATTTCCTGACCGGCAACCGCGACCCCGATGATCACCGACTGACCCCAGCCGCGGTGCGCACTTTCCAGCGCCGCACGCATCACGTTGACGTTACCGATGCATTCAAAGGTATGGTCGATACCCCATTTGTTGATATCCAACAGGACATCTTTTATCGGTTTGTCGTAGTCATTCGGGTTAATGCAGTCGGTAGCACCGAAGCGACGCGCCAGATCGA > NC_000913/378643‑378901 | ttCCATACGCGACCAGTGACCAACTGGGATGGACGGGTGGAGATTTCCTGACCGGCAACCGCGACCCCGATGATCGCCGACTGACCCCAGCCGCGGTGCGCACTTTCCAGCGCCGCACGCATCACGTTGGCGt > 1:328581/1‑133 (MQ=255) cgcgACCAGTGACCAGCTCGAATGGACGGGTGGAGATTTCCTGACCGGCAACCGCGACCCCGATGATCACCGACTGACCCCAGCCGCGGTGCGCACTTTCCAGCGCCGCACGCATCACGTTGGCGTTACCGAt < 1:205879/133‑1 (MQ=255) gACCAGTGACCAACTGGAATGGACGGGTGGAGATTTCCTGACCGGCAACCGCGACCCCGATGATCACCGACTGACCCCAGCCGCGGTGCGCACTTTCCAGCGCCGCACGCATCACGTTGGCGTTACCGATGCa > 1:102632/1‑133 (MQ=255) aCCAGTGACCAACTGGAATGGGCGGGTGGAGATTTCCTGACAGGCAACCGCGACCCCGAAGATCACCGACTGACCCCACCCGCGCTGCGCACTTTCCAGCGCCGCACGCATCACGTTGGCGTTACCCATGCAt > 2:317901/1‑133 (MQ=255) gTGACCAACTCGAATTGACGGGTGTAGACTTCTTGACCGGCAACCGCGACCCCGATGATCACCGACTGACCCCAGCCGCGGTGCGCACTTTCCAGCGCCGCACGCATCACGTTGGCGTTACCGATGCATTCaa < 2:130419/133‑1 (MQ=255) aCTGGAATGGACGGGTGGAGATTTCCTGACCGGCAACCGCGACCCCGATGATCACCGACTGACCCCAGCCGCGGTGCGCACTTTCCAGCGCCGCACGCATCACGTTGGCGTTACCGATGCATTCAAAGGTATg < 2:3330/133‑1 (MQ=255) gAATGGACGGGTGGAGATTTCCTGACCGGCAACCGCGACCCCGATGATCACCGACTGACCCCAGCCGCGGTGCGCACTTTCCAGCGCCGCACGCATCACGTTGGCGTTACCGATGCATTCAAAGGTATGGTCg > 2:127831/1‑133 (MQ=255) aTGGACGGGTGGAGATTTCCTGACCGGCAACCGCGACCCCGATGATCACCGACTGACCCCAGCCGCGGTGCGCACTTTCCAGCGCCGCACGCATCACGTTGGCGTTACCGATGCATTCAAAGGTATGGTCGAt > 1:2297/1‑133 (MQ=255) ggACGGGTGGAGATTTCCTGACCGGCAACCGCGACCCCGATGATCACCGACTGACCCCAGCCGCGGTGCGCACTTTCCAGCGCCGCACGCATCACGTTGGCGTTACCGATGCATTCAAAGGTATGGTCGATAc > 1:308172/1‑133 (MQ=255) gggTGGAGATTTCCTGACCGGCAACCGCGACCCCGATGATCACCGACTGACCCCAGCCGCGGTGCGCACTTTCCAGCGCCGCACGCATCACGTTGGCGTTACCGATGCATTCAAAGGTATGGTCGATACCCCa < 1:127832/133‑1 (MQ=255) aCCCCGATGATCACCGACTGACCCCAGCCGCGGTGCGCACTTTCCAGCGCCGCACGCATCACGTTGGCGTTACCGATGCATTCAAAGGTATGGTCGATACCCCATTTGTTGATATCCAACAGGACATCTTTTa < 1:127831/133‑1 (MQ=255) gatgatCACCGACTGACCCCAGCCGCGGTGCGCACTTTCCAGCGCCGCACGCATCACGTTGGCGTTACCGATGCATTCAAAGGTATGGTCGATACCCCATTTGTTGATATCCAACAGGACATCTTTTATCGGt > 1:370335/1‑133 (MQ=255) gacgatCGCCGACTGACCCCAGCCGCGGTGCGCACTTTCCAGCGCCGCACGCATCACGTTGGCGTTACCGATGCATTCAAAGGTATGGTCGATACCCCATTTGTTGATATCCAACAGGACATCTTTTATCGGt < 2:328581/130‑1 (MQ=255) aCCCCAGCCGCGGTGCGCACTTTCCAGCGCCGCACGCATCACGTTGGCGTTACCGATGCATTCAAAGGTATGGTCGATACCCCATTTGTTGATATCCAACAGGACATCTTTTATCGGTTTGTCGTAGTCATTc > 2:366555/1‑133 (MQ=255) ccccAGCCGCGGTGCGCACTTTCCAGCGCCGCACGCATCACGTTGGCGTTACCGATGCATTCAAAGGTATGGTCGATACCCCATTTGTTGATATCCAACAGGACATCTTTTATCGGTTTGTCGTAGTCATTCg > 2:76349/1‑133 (MQ=255) ggTGCGCACTTTCCAGCGCCGCACGCATCACGTTGGCGTTACCGATGCATTCAAAGGTATGGTCGATACCCCATTTGTTGATATCCAACAGGACATCTTTTATCGGTTTGTCGTAGTCATTCGGGTTAATGCa < 2:102632/133‑1 (MQ=255) ccgcACGCATCACGTTGGCGTTACCGATGCATTCAAAGGTATGGTCGATACCCCATTTGTTGATATCCAACAGGACATCTTTTATCGGTTTGTCGTAGTCATTCGGGTTAATGCAGTCGGTAGCACCGAAGCg < 2:308172/133‑1 (MQ=255) cacgcaTCACGTTGGCGTTACCGATGCATTCAAAGGTATGGTCGATACCCCATTTGTTGATATCCAACAGGACATCTTTTATCGGTTTGTCGTAGTCATTCGGGTTAATGCAGTCGGTAGCACCGAAGCGAcg > 2:49398/1‑133 (MQ=255) ttGGCGTTACCGATGCATTCAAAGGTATGGTCGATACCCCATTTGTTGATATCCAACAGGACATCTTTTATCGGTTTGTCGTAGTCATTCGGGTTAATGCAGTCGGTAGCACCGAAGCGACGCGCCAGATCGa < 2:2297/133‑1 (MQ=255) | TTCCATACGCGACCAGTGACCAACTGGAATGGACGGGTGGAGATTTCCTGACCGGCAACCGCGACCCCGATGATCACCGACTGACCCCAGCCGCGGTGCGCACTTTCCAGCGCCGCACGCATCACGTTGACGTTACCGATGCATTCAAAGGTATGGTCGATACCCCATTTGTTGATATCCAACAGGACATCTTTTATCGGTTTGTCGTAGTCATTCGGGTTAATGCAGTCGGTAGCACCGAAGCGACGCGCCAGATCGA > NC_000913/378643‑378901 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |