Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	410,100	T→C	intergenic (‑81/‑44)	rdgC ← / → mak	nucleoid‑associated protein RdgC/manno(fructo)kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	410,100	0	T	C	100.0%	119.5 / NA	35	intergenic (‑81/‑44)	rdgC/mak	nucleoid‑associated protein RdgC/manno(fructo)kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/24);  total (11/24)

TCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTACCGTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGATTGCACTGGGCGATGCAGGGGAGCAGTTGTACCGCCATCG  >  NC_000913/409974‑410226                                                                                                                               |                                                                                                                               tctcGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGtct                                                                                                                          <  1:338866/133‑1 (MQ=255) tctcGCGGCTAAGACAGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGtct                                                                                                                          <  1:253032/133‑1 (MQ=255)   tcGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTctct                                                                                                                        <  2:363863/133‑1 (MQ=255)     gcgGCTAAGACGGTATACGATTTAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTAc                                                                                                                      <  1:239092/133‑1 (MQ=255)     gcgGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTAc                                                                                                                      <  2:394563/133‑1 (MQ=255)     gcgGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTAc                                                                                                                      <  1:473273/133‑1 (MQ=255)      cgGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTa                                                                                                                       >  2:73786/1‑131 (MQ=255)      cgGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTa                                                                                                                       <  1:73786/131‑1 (MQ=255)      cgGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTGGCATTGCAAATGCGGTTGCCCGTCTCTACg                                                                                                                     >  1:144162/1‑133 (MQ=255)               cGGTAAACCATTAAATTTGTGACCCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCtattgata                                                                                                            <  1:225297/133‑1 (MQ=255)                       cATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGaaaaa                                                                                                    >  1:458622/1‑133 (MQ=255)                       cATTAAATTTTTGAAACACAGCATAATAATTTGCACGGCCTGGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGaaaaa                                                                                                    <  2:227136/133‑1 (MQ=255)                          tAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAAt                                                                                                 <  1:42555/133‑1 (MQ=255)                          tAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAAt                                                                                                 <  1:311955/133‑1 (MQ=255)                          tAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAAt                                                                                                 <  2:458622/133‑1 (MQ=255)                                    aaCCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTa                                                                                       >  2:101973/1‑133 (MQ=255)                                      caacaGCATGTTAATTTCCACGGGCTGGTCGTCAAATTTAGTGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTAcc                                                                                     <  2:250041/131‑1 (MQ=255)                                              tGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTACCGTGCGtat                                                                             >  2:60914/1‑133 (MQ=255)                                              tGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGGCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTACCGTGCGtat                                                                             <  1:500407/133‑1 (MQ=255)                                              tGATAATTGCCACGGTCTAGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTACCGTGCGtat                                                                             <  1:241923/133‑1 (MQ=255)                                                              cTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTACCGTGCGTATAGGTATCGATTTAggc                                                             <  1:504481/133‑1 (MQ=255)                                                                tGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTACAGTGCGTATAGGTATCGATTTAggcgg                                                           >  1:427139/1‑133 (MQ=255)                                                                 gTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTACCGTGCGTATAGGCATCGATTTAggcggc                                                          >  1:121493/1‑133 (MQ=255)                                                                   cGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTACCGTGCGTATAGGTATCGATTTAGGCGGCAc                                                        <  1:12011/133‑1 (MQ=255)                                                                                 ggCATGATAATGAATAGTCGGCGGCCTTTCATTGCCAATCCCGTTGCCCGTCTCTACGGTATTGATATTGAAAAAAATAAGGAGAGTACCGTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGa                                          <  2:144162/133‑1 (MQ=255)                                                                                 ggCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTACCGTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGa                                          <  2:430681/133‑1 (MQ=255)                                                                                      gATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTACCGTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGATTGCa                                     <  2:326664/133‑1 (MQ=255)                                                                                           cGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTACCGTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGATTGCACTggg                                >  1:267548/1‑133 (MQ=255)                                                                                            gAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTACCGTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGa                                          >  1:493930/1‑122 (MQ=255)                                                                                            gAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTACCGTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGa                                          <  2:493930/122‑1 (MQ=255)                                                                                              aTTGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTACCGTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGATTGCACTGGGCGa                             <  1:60914/133‑1 (MQ=255)                                                                                                tGTCGGCGGCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTACCGTGCGTATAGGTATCGATTTAGGCGGCACCAAAACCGAAGTGATTGCACTGGGCGATg                           >  1:230286/1‑133 (MQ=255)                                                                                                   cggcggCCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTACAGTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGATTGCACTGGGCGATGCAg                        <  2:427139/133‑1 (MQ=255)                                                                                                      cggcCTTGCACTGCCTATCCGGTTACCCGTCTTTACGCTATTGATATTGAAAAAAATAAGGAGAGTACCGTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGATTGCACTGGGCGATGCAgggg                     <  2:196115/133‑1 (MQ=255)                                                                                                       ggcCTTGCATTGCCAATCCGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTACCGTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGATTGCACTGGGCGATGCAGGGGa                    >  2:29625/1‑133 (MQ=255)                                                                                                                         cGGTTGCCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTACCGTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGATTGCACTGGGCGATGCAGGGGAGCAGTTGTACCGCCATCg  <  2:276707/133‑1 (MQ=255)                                                                                                                               |                                                                                                                               TCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTCCGTCTCTACGCTATTGATATTGAAAAAAATAAGGAGAGTACCGTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGATTGCACTGGGCGATGCAGGGGAGCAGTTGTACCGCCATCG  >  NC_000913/409974‑410226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑