| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 415,162 | T→C | T198A (ACA→GCA) | sbcC ← | ATP dependent, structure specific DNA nuclease ‑ SbcC subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 415,162 | 0 | T | C | 87.5% | 35.4 / ‑0.8 | 16 | T198A (ACA→GCA) | sbcC | ATP dependent, structure specific DNA nuclease ‑ SbcC subunit |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (5/9); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.17e-01 | |||||||||||
TGCTGCTGCGCGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCT > NC_000913/415032‑415253 | tgctgctgcGCGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTgcgc > 1:94833/1‑133 (MQ=255) gctgctgcGCGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGcgcg > 2:17746/1‑133 (MQ=255) gctgctgcGCGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGcgcg > 1:400920/1‑133 (MQ=255) tttttCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCGGCTATACCCGGGCCGATTTGTTATGCTCaaaa > 2:253832/1‑133 (MQ=255) taCCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGCGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGc < 2:94833/133‑1 (MQ=255) aCCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGCGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGcc > 2:392136/1‑133 (MQ=255) cAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGCGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAg > 1:344148/1‑133 (MQ=255) cTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGCGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGt < 1:392136/133‑1 (MQ=255) tGTCAGCGGTTGCACATGTTCCGGCGTGAGTAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGCGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTg < 1:253832/133‑1 (MQ=255) tGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGCGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTg < 1:491824/133‑1 (MQ=255) tCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGCGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGcc < 2:400920/133‑1 (MQ=255) cACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGCGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAActcc < 2:344148/133‑1 (MQ=255) tCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGCGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGGGATTTGCCAGTAGATTTCAGTGCCGTTTAACTCCTCGAGCa > 2:205708/1‑133 (MQ=255) gAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGCGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCcgcg < 1:342921/133‑1 (MQ=255) cGTGACGCCGCTGGCGTGCGCTTGCAGCTTCTCCAGCTCTGCGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCt < 1:167309/133‑1 (MQ=255) cGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGCGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCcgcg > 1:493018/1‑127 (MQ=255) cGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGCGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCcgcg < 2:493018/127‑1 (MQ=255) tGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCt < 1:40318/122‑1 (MQ=255) tGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCt > 2:40318/1‑122 (MQ=255) | TGCTGCTGCGCGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCT > NC_000913/415032‑415253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |