Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	420,893	A→G	T435A (ACC→GCC)	brnQ →	branched chain amino acid transporter BrnQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	420,893	0	A	G	100.0%	53.9 / NA	17	T435A (ACC→GCC)	brnQ	branched chain amino acid transporter BrnQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/9);  total (8/9)

AGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCG  >  NC_000913/420767‑421011                                                                                                                               |                                                                                                                       aGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGGCCTCCa                                                                                                                  <  2:351699/133‑1 (MQ=255)  gCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGGCCTCCAg                                                                                                                 <  2:272649/133‑1 (MQ=255)    gATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGACGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGGCCTCCAgcg                                                                                                               <  2:469289/133‑1 (MQ=255)      tatCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGGCCTCCAGCGCt                                                                                                             >  2:389944/1‑133 (MQ=255)      tatCTTACCGTCCTGGGCGCAGCGCTTAACGCTGGCCGAACGAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGGCCTCCAGCGCt                                                                                                             <  2:167225/133‑1 (MQ=255)                        gcAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGGCCTCCAGCGCTCACTAAATCACTGAACAt                                                                                           <  1:389944/133‑1 (MQ=255)                            cGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGGCCTCCAGCGCTCACTAAATCACTGAACATTTGt                                                                                       <  1:36376/133‑1 (MQ=255)                             gTTTACCGCTTGCCGGACAAGGTCTGGCTTGGCTAATGCCACCAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGGCCTCCAGCGCTCACTAAATCACTGAACATTTGtt                                                                                      <  2:185962/133‑1 (MQ=255)                                                           ggTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGGCCTCCAGAGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGtt                                                        >  2:52507/1‑133 (MQ=255)                                                                      aCAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGGCCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTgtgt                                             >  1:347677/1‑133 (MQ=255)                                                                      aCAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGGCCTCCAGCGCTCACTAAATCACTGAACATTTGTATTAACCACTGGGATGCAATGCCCCGTGGTTTTTTATTgtgt                                             >  2:206793/1‑133 (MQ=255)                                                                                     gtTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGGCCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGATGATGGGTTAGGAAt                              >  2:214252/1‑133 (MQ=255)                                                                                      ttCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGGCCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAAtt                             >  1:376396/1‑133 (MQ=255)                                                                                           gCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGGCCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATgg                        >  1:255220/1‑133 (MQ=255)                                                                                                      ggATCGCGCGGCAGGTCGTCAGGTGGCCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAAc             <  1:52507/133‑1 (MQ=255)                                                                                                            cgcgGCAGGTCGTCAGGTGGCCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTa       >  1:269228/1‑133 (MQ=255)                                                                                                                 cAGGTCGTGAGGTGGCCTTCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCg  <  1:206793/133‑1 (MQ=255)                                                                                                                               |                                                                                                                       AGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCG  >  NC_000913/420767‑421011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑