| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 433,447 | A→G | D149G (GAC→GGC) | nrdR → | NrdR transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 433,447 | 0 | A | G | 93.3% | 44.6 / NA | 15 | D149G (GAC→GGC) | nrdR | NrdR transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (2/12); total (3/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGATTGGCAATCTGGTGATGGAGCAATTGAAAAAGCTCGATAAAGTCGCCTATATCCGTTTTGCCTCTGTCTACCGCAGTTTCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGT > NC_000913/433319‑433543 | gatgatTGGCAATCTGGTGATGGAGCAATTGAAAAAGCTCGATAAAGTCGCCTATATCCGTTTTGCCTCTGTCTACCGCAGTTTCGAAGATATCAAAGAATTGGGCGAAGAGATCGCTCGCCTGGAGGGCTaa > 1:250344/1‑133 (MQ=255) atgatTGGCAATCTGGTGATGGAGCAATTGAAAAAGCTCGATAAAGTCGCCTATATCCGTTTTGCCTCTGTCTACCGCAGTTTCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGGCTAAg < 1:88207/133‑1 (MQ=255) tGGCAATCTGGTGATGGAGCAATTGAAAAAGCTCGATAAAGTCGCCTATATCCGTTTTGCCTCTGTCTACCGCAGTTTCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGGCTAAGCCGTg < 2:77818/133‑1 (MQ=255) cAATCTGGTGATGGAACAATTGAATAAGTTCGGTAAAGTCGTCTATATCCGTTTTGCCTCTGTCTACCGCAGTTTCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGGCTAAGCCGTGCAg < 2:208321/133‑1 (MQ=255) aaTCTGGTGATGGAGAAATTGAAACAGCCCGAAAAAGTCGCCTATATCCGTTTTGCCTCTGTCTACCGCAGTTTCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGGCTAAGCCGTGCAgg < 2:136706/133‑1 (MQ=255) aTCTGGTGATGGAGCAATTGAAAAAGCTCGATAAAGTCGCCTATATCCGTTTTGCCTCTGTCTACCGCAGTTTCGAAGATATCAAAGAATTTGGCGAAGAGAGCGCGCGCGTGGGGGACTAAGCGGTGCAGGa > 1:136706/1‑133 (MQ=255) tCTGGTGATGGAGCAATTGAAAAAGCTCGATAAAGTCGCCTATATCCGTTTTGCCTCTGTCTACCGCAGTTTCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGGCTAAGCCGTGCAGGAc < 2:337289/133‑1 (MQ=255) cTGGTGATGGAGCAATTG‑AAAAGCTCGATAAAGTCGCCTATATCCGTTTTGCCTCTGTCTACCGCAGTTTCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGGCTAAGCCGTGCAGGACGa < 2:73453/133‑1 (MQ=255) gAAAAAGCTCGATAAAGTCGCCTATATCCGTTTTGCCTCTGTCTACCGCAGTTTCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGGCTAAGCCGTGCAGGACGAGTATTACATGGCGCgg < 1:428659/133‑1 (MQ=255) atatCCGTTTTGCCTCTGTCTACCGCAGTTTCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGGCTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAgg < 1:454212/133‑1 (MQ=255) ttttGCCTCTGTCTACCGCAGTTTCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGGCTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGtttt > 1:478637/1‑133 (MQ=255) tatGCCTCTCCCTACTGCAGTTTCGAAGTTATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGGCTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTa < 2:250344/131‑1 (MQ=255) ccTCTGTCTACCGCAGTTTCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGGCTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCAc < 1:395076/133‑1 (MQ=255) ctctGTCTACCGCAGTTTCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGGCTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACg < 1:68467/133‑1 (MQ=255) tCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGGCTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGt < 2:304836/133‑1 (MQ=255) | GATGATTGGCAATCTGGTGATGGAGCAATTGAAAAAGCTCGATAAAGTCGCCTATATCCGTTTTGCCTCTGTCTACCGCAGTTTCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGT > NC_000913/433319‑433543 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |