Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	473,912	C→T	A316V (GCG→GTG)	amtB →	ammonia/ammonium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	473,912	0	C	T	100.0%	24.8 / NA	11	A316V (GCG→GTG)	amtB	ammonia/ammonium transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/5);  total (6/5)

TGGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCCCTGCGATGTCTTCGGTGTGCACGGCGTTTGTGGCATTGTCGGCTGTATC  >  NC_000913/473807‑474012                                                                                                          |                                                                                                     tgGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTGACGCCACCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGTGGGCTTGTGGGGCGTTACCATGCTCaa                                                                           >  2:481940/1‑133 (MQ=255)             atGGCGCGATCGCCGGTCTGGTCGGCGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGTGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCg                                                               <  2:121171/132‑1 (MQ=255)               ggCGCGATTGCCGGTCTGGTCGGCGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGTGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCggg                                                             <  1:384832/133‑1 (MQ=255)                   cgATTGCCGGTCTGGTCGGCGTGACGCCACCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGTGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGga                                                         <  1:481940/133‑1 (MQ=255)                    gATTGCCGGTCTGGTCGGCGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGTGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGgat                                                        <  1:363440/133‑1 (MQ=255)                     aTTGCCGGTCTGGTCGGCGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGTGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGgatg                                                       >  1:114920/1‑133 (MQ=255)                                      cGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGTGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGCGGATGATCCCTGCGATGTCTTc                                      >  1:257179/1‑133 (MQ=255)                                                         tACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGTGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCCCTGCGATGTCTTCGGTGTGCACGGCGTTtgtg                   <  1:386156/133‑1 (MQ=255)                                                                     ggcggcGCGTTGATTATCGGCGTGGTAGCTGGTCTGGTGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCCCTGCGATGTCTTCGGTGTGCACGGCGTTTGTGGCATTGTCGGCt       >  1:412742/1‑133 (MQ=255)                                                                     ggcggcGCGTTGATTATCGGCGTGGTAGCTGGTCTGGTGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCCCTGCGATGTCTTCGGTGTGCACGGCGTTTGTGGCATTGTCGGCt       >  2:484350/1‑133 (MQ=255)                                                                          cgcgTTGATTATCGGCGTGGTAGCTGGTCTGGTGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCCCTGCGATGTCTTCGGTGTGCACGGCGTTTGTGGCATTGTCGGCTGTATc  >  2:96361/1‑133 (MQ=255)                                                                                                          |                                                                                                     TGGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCCCTGCGATGTCTTCGGTGTGCACGGCGTTTGTGGCATTGTCGGCTGTATC  >  NC_000913/473807‑474012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑