Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	476,031	A→G	V114A (GTA→GCA)	atl ←	DNA base‑flipping protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	476,031	0	A	G	100.0%	61.2 / NA	19	V114A (GTA→GCA)	atl	DNA base‑flipping protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/8);  total (11/8)

CTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAACCACCCGGTGCCAGGGTAAGGTGCTGCCTTCAGGGAGACG  >  NC_000913/475905‑476152                                                                                                                               |                                                                                                                          cTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATGCCATca                                                                                                                     <  1:274658/133‑1 (MQ=255)  tCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCAGCTTCCCGACGCCATcac                                                                                                                    >  1:214285/1‑133 (MQ=255)      gTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATGCCATca                                                                                                                     <  2:60697/128‑1 (MQ=255)      gTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATGCCATca                                                                                                                     >  1:60697/1‑128 (MQ=255)         aaTCCGTCTTCGGCATTTCCAGGTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATGCCATCACACCTTCt                                                                                                             <  1:198649/133‑1 (MQ=255)           tCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATGCCATCACACCTTCTGc                                                                                                           >  2:109611/1‑133 (MQ=255)           tCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATGCCATCACACCTTCTGc                                                                                                           >  2:316066/1‑133 (MQ=255)                                                gCGACGTTCACCCTCTCCGCCCGGAGAGGGCTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATGCCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtccggtccg                                                                      <  1:217700/133‑1 (MQ=255)                                                     gTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATGCCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAg                                                                 >  2:313704/1‑133 (MQ=255)                                                                  gCCCGGAGAGAGTTAATAGTTCCACCGAAAACTCTGCAAGTCGATTTGCCCGCTTCCCGATGCCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCg                                                    <  2:214285/133‑1 (MQ=255)                                                                     cGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATGCCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTgg                                                 <  1:316066/133‑1 (MQ=255)                                                                       gagaGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATGCCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCg                                               <  1:313704/133‑1 (MQ=255)                                                                                    gtTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATGCCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAACCACCCgg                                  >  1:490429/1‑133 (MQ=255)                                                                                      tCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATGCCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAACCACCCGGTg                                >  1:23483/1‑133 (MQ=255)                                                                                      tCCAGCGATAACGCTGCAAGTCGATTTGCCCACTTCCCGATGCCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGGCCGGTTAGAGAAATTGTGCCGTGGCGATTATCCACCCGGTg                                >  2:131952/1‑133 (MQ=255)                                                                                       ccAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATGCCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTGCGATTAGCTAAAGTGTACCGTGGCGATTAACCACCCGGTGc                               >  2:140581/1‑133 (MQ=255)                                                                                                       aaGTCGATTTGCCCGCTTCCCGATGCCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGGGCCGTGGCGATTAACCTCACGGTGCGAGGGGAAGGtgctgc               >  2:145863/1‑133 (MQ=255)                                                                                                                tGCCCGCTTCCCGATGCCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGCAAATCCGGCACGGTTAGCGAAATTGTGCCGTGGCGATTAACCACCCGGTGCCAGGGTAAGGTGCTGCCTTCAGGga      >  1:279348/1‑133 (MQ=255)                                                                                                                    cGCTTCCCTATGCCAACACACCCTCTGCCATTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAACCACCCGGTGCCAGGGTAAGGTGCTGCCTTCAGGGAGACg  <  1:145863/133‑1 (MQ=255)                                                                                                                               |                                                                                                                          CTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAACCACCCGGTGCCAGGGTAAGGTGCTGCCTTCAGGGAGACG  >  NC_000913/475905‑476152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑