| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 523,668 | T→C | S136S (AGT→AGC) | rhsD → | protein RhsD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 523,668 | 0 | T | C | 85.7% | 13.7 / ‑5.6 | 8 | S136S (AGT→AGC) | rhsD | protein RhsD |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (3/3); total (3/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGAGCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGCGCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCG > NC_000913/523557‑523785 | ttCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGAGCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGCGAGTCAATGTGGCTGGTgcgc < 2:108142/133‑1 (MQ=255) cACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGCGAGTCAATGTGGCTGGTGCGCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGata > 1:55428/1‑133 (MQ=255) ttGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGCGAGTCAATGTGGCTGGTGCGCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCg > 1:18032/1‑133 (MQ=255) gAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGCGAGTCAATGTGGCTGGTGCGCGGTGGTAAGGCAGCACAGCCGGACGACCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGt < 2:286206/133‑1 (MQ=255) ctgctgCCGGGGGAGGCGGTGTACAGCCGCAGCGAGTCAATGTGGCTGGTGCGCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGcc < 2:399605/133‑1 (MQ=255) ggCGGTGTTCAGCCGCAGTGAGGCAATGTGTCTGGTGCGCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTg < 1:204106/133‑1 (MQ=255) cGGTGTACAGCCGCAGCGAGTCAATGTGGCTGGTGCGCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCAGTTAAGCCGGCATATTTACCTGGc > 2:204106/1‑133 (MQ=255) ggTGTACATCCGCAGGGAGTCATTGTGGCTGGTGCGCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCg < 1:172319/133‑1 (MQ=255) | TTCTGATATCCGCTTACAGCTACGTGATGACGGACTGATACTCAACGACAACGGCGGGCGGAGCATTCACTTTGAGCCGCTGCTGCCGGGGGAGGCGGTGTACAGCCGCAGTGAGTCAATGTGGCTGGTGCGCGGTGGTAAGGCAGCACAGCCGGACGGCCATACGCTGGCGCGGCTGTGGGGGGCGCTGCCGCCGGATATCCGGTTAAGCCCGCATCTTTACCTGGCG > NC_000913/523557‑523785 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |