| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 585,935 | A→G | intergenic (‑302/+212) | ompT ← / ← envY | outer membrane protease VII (outer membrane protein 3b)/DNA‑binding transcriptional activator EnvY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 585,935 | 0 | A | G | 97.0% | 108.7 / ‑3.1 | 33 | intergenic (‑302/+212) | ompT/envY | outer membrane protease VII (outer membrane protein 3b)/DNA‑binding transcriptional activator EnvY |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (11/21); minor base . (0/1); total (11/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
TACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGG > NC_000913/585806‑586054 | tACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCaa > 2:368777/1‑133 (MQ=255)tACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCaa < 1:496518/133‑1 (MQ=255) aCTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCaaa > 1:474618/1‑133 (MQ=255) tgGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTgag < 1:18479/133‑1 (MQ=255) tgGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTgag > 2:443945/1‑133 (MQ=255) tgGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTgag < 1:47858/133‑1 (MQ=255) tgGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCAATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTgag > 1:441400/1‑133 (MQ=255) tttATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATa < 2:69878/133‑1 (MQ=255) tgtgGTGCTTTTATCTATTTAAGTGCAATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCa < 2:441400/133‑1 (MQ=255) ttttATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTg < 2:64924/133‑1 (MQ=255) ttATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCa < 1:414387/133‑1 (MQ=255) tATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCt > 1:263229/1‑133 (MQ=255) ttAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACGTAAGGCCGCGCAATAGCTTGCAAGGCTTTa > 1:249128/1‑133 (MQ=255) tAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGG‑CAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACa < 1:1172/133‑1 (MQ=255) tAAGTGCCATGCCTTTAGAGGCATATAAGCAAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTAc < 2:270940/133‑1 (MQ=255) aaGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACa < 1:44356/133‑1 (MQ=255) aaGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACa < 1:68156/133‑1 (MQ=255) gTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATg < 1:443945/133‑1 (MQ=255) aagCCTTTAGAGGCACATAAGCGAAATTTGCACGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTg < 2:249128/131‑1 (MQ=255) aTGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTg < 2:474618/133‑1 (MQ=255) ttAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAg < 2:406743/133‑1 (MQ=255) ttAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAg < 1:11249/133‑1 (MQ=255) atatAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACgt > 1:477/1‑133 (MQ=255) atatAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACgt > 1:332984/1‑133 (MQ=255) ataAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACgtgt < 2:263229/133‑1 (MQ=255) gCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATg < 1:480725/133‑1 (MQ=255) cATGAGGTTTATCCTCAATTACTATGTTTTTTACAACAAAAATGAGGGGCAAAACTGAGACACATAAGCCGTTGCAATGGCTTACAAGGCTTAACTTGTTTTAAGGAAGTGGGACGTGTGAGCCCAGAGAttg > 2:83359/1‑131 (MQ=255) aTGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGgc < 1:368777/133‑1 (MQ=255) aaTTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGa > 2:383254/1‑133 (MQ=255) aaTTACTATGTTTTTTAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGa > 2:469815/1‑133 (MQ=255) cTATGTTTTTTAGTAAAACAAAGAGGGGCACAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTaa < 1:200430/133‑1 (MQ=255) ttttAGTACAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCg > 2:327267/1‑133 (MQ=255) aCAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGTGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAgg < 2:332984/133‑1 (MQ=255) aCAAAAAAGAGGGGCAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAgg < 1:64155/133‑1 (MQ=255) | TACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGG > NC_000913/585806‑586054 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |