| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 593,357 | T→C | T472A (ACA→GCA) | cusS ← | sensory histidine kinase CusS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 593,357 | 0 | T | C | 94.3% | 104.9 / ‑2.1 | 35 | T472A (ACA→GCA) | cusS | sensory histidine kinase CusS |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (19/14); total (20/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
AGGTTTTTTATGAATTGTGACAGCTTTTTACCATTAATAGGTATGACTATTGCGGCACGTTATTTTTACACTGGTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACG > NC_000913/593227‑593483 | aGGTTTTTTATGAATTGTGACAGCTTTTTACCATTAATAGGTATGACTATTGCGGCACGTTATTTTTACACTGGTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGccc > 2:459709/1‑133 (MQ=255) ttttATGAATTGTGACAGCTTTTTACCATTAATAGGTATGACTATTGCGGCACGTTATTTTTACACTGGTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCcgc > 2:295988/1‑133 (MQ=255) tttATGAATTGTGACAGCTTTTTACCATTAATAGGTATGACTATTGCGGCACGTTATTTTTACACTGGTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTGATGTGATAACAAACCTTGCCCCCcgcg > 1:264854/1‑133 (MQ=255) ttGTGACAGCCTTTTACCATTAATTGGTTTGACTATTGCGGTACGTTATTTTTACACTGGTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGAc < 2:181657/133‑1 (MQ=255) ttttACCATTAATAGGTATGACTATTGCGGCACGTTATTTTTACACTGGTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAAc > 1:337156/1‑133 (MQ=255) tATGACTATTGCGGCACGTTATTTTTACACTGGTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCAGCCGGCGTCACCGCAACTGTGCCTTTATGcgcg > 2:192784/1‑133 (MQ=255) tGACTATTGCGGCACGTTATTTTTACACTGGTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGac > 1:26529/1‑133 (MQ=255) aCTATTGCAGCACGTTTTATTTTCCCTGGTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGacaa < 1:209835/133‑1 (MQ=255) ttGCGGCACGTTATTTTTACACTGGTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCGTCCGACGTAACCACAAACGTGCCTTGATGCACGACAACAAt > 2:148382/1‑133 (MQ=255) tGCGGCACGTTATTTTTACACTGGTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATc > 1:417072/1‑133 (MQ=255) cACGTTATTTTTACACTGGTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGAttt > 1:383329/1‑133 (MQ=255) cGTTATTTTTACACTGGTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGACGCTACCGCAACCGTGCTTATATGCGCGGCAACTATCGATTTCa > 2:227192/1‑133 (MQ=255) tttACACTTGTTATACAATTTGGCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGc < 1:192784/133‑1 (MQ=255) tttACACTTGTTATACAACTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGc < 1:148382/133‑1 (MQ=255) tttACACTGGTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAACCCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGc < 1:36381/133‑1 (MQ=255) tttACACTGGTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGc < 2:26529/133‑1 (MQ=255) tttACACTGGTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGc < 2:417072/133‑1 (MQ=255) tttACACTGGTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGc < 1:26047/133‑1 (MQ=255) acacTGGTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAg > 2:81099/1‑133 (MQ=255) ggTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGc > 2:378172/1‑125 (MQ=255) ggTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGc < 1:378172/125‑1 (MQ=255) ggTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCaa < 2:383329/133‑1 (MQ=255) ggTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCaa > 2:363688/1‑133 (MQ=255) taAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCg < 1:81099/133‑1 (MQ=255) ttGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCt > 2:4099/1‑133 (MQ=255) ttGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCt > 1:344833/1‑133 (MQ=255) ttGCCGTTTCTTGAAGGATTAAGTAGGTAATGCGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCt < 2:244016/133‑1 (MQ=255) aaGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGa > 2:313207/1‑133 (MQ=255) aaGCGGGTAATGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGa > 2:401455/1‑133 (MQ=255) aaTGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCaa < 1:401455/133‑1 (MQ=255) aaTGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCaa < 1:363688/133‑1 (MQ=255) aaTGTGATAACAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCaa < 1:295988/133‑1 (MQ=255) aaCAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAg > 1:96469/1‑133 (MQ=255) aCAAACCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGa < 2:344833/133‑1 (MQ=255) aCAAAACTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAgg > 1:389127/1‑132 (MQ=255) aaCCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAAc > 2:502694/1‑133 (MQ=255) aCCTTGCCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACg > 1:485181/1‑133 (MQ=255) | AGGTTTTTTATGAATTGTGACAGCTTTTTACCATTAATAGGTATGACTATTGCGGCACGTTATTTTTACACTGGTTATAAAAGTTGCCGTTTGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACG > NC_000913/593227‑593483 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |