Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	617,030	A→G	L958L (TTA→TTG)	entF →	apo‑serine activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	617,030	0	A	G	100.0%	74.9 / NA	23	L958L (TTA→TTG)	entF	apo‑serine activating enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/11);  total (12/11)

TTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCG  >  NC_000913/616905‑617159                                                                                                                              |                                                                                                                                  ttGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACACTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACCTAGCGCCAGCGGAAAGATGGATCGCTAAGcgttgccgttgc                                                                                                                            >  2:144007/1‑133 (MQ=255)   ggATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCct                                                                                                                          >  1:22369/1‑133 (MQ=255)                      gcgcAGCTTCGTGAAACATTGTCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAgcgc                                                                                                       >  1:432620/1‑133 (MQ=255)                       cgcAGCTTCGGGAAACATTGCCACCACATATGTTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGcc                                                                                                      <  2:171557/133‑1 (MQ=255)                        gcAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGCCa                                                                                                     >  2:9688/1‑133 (MQ=255)                                  gAAGCATTGCCACCATATACGGTAACGGAGGTCCTGCTGCAGCTGCCGCAGTTACCGCTTAGCGCCACCGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTgcgc                                                                                           <  2:196165/133‑1 (MQ=255)                                       actGCCACCACATATCGTCCCGCTGGTTCTTCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGaaa                                                                                      <  1:144007/131‑1 (MQ=255)                                                acaTATGATACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGt                                                                             >  1:507609/1‑133 (MQ=255)                                                    atGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGCGCGCCGAAAGCGGTCAGTGaaa                                                                         >  1:225359/1‑133 (MQ=255)                                                         aCCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGt                                                                             <  1:369267/124‑1 (MQ=255)                                                         aCCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGt                                                                             >  2:369267/1‑124 (MQ=255)                                                            ggtggtTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTGAAACGATTATc                                                                 >  1:341358/1‑133 (MQ=255)                                                                gtTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTGAAACGATTATcgccg                                                             <  1:385725/133‑1 (MQ=255)                                                                            cTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCtcgt                                                 <  1:9688/133‑1 (MQ=255)                                                                              tCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCTGTGGAACGATTATCGCAGCGGGATTCtcgtcg                                               >  2:212348/1‑133 (MQ=255)                                                                                   aGTTACCACTTAGCGCCAACGGGAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCt                                          <  1:212348/133‑1 (MQ=255)                                                                                       aCCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTgggg                                      >  1:432923/1‑133 (MQ=255)                                                                                       aCCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTGAAACGATCATCGCCGAGGCATTCTCGTCGGTGCTgggg                                      >  1:164640/1‑133 (MQ=255)                                                                                             tAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGAc                                <  1:510360/133‑1 (MQ=255)                                                                                              aGCGCCAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCGTTGTCGGCGGTGCTGGTGTGTGACg                               >  1:165236/1‑133 (MQ=255)                                                                                              aGCGCCAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCt                                          >  2:292475/1‑122 (MQ=255)                                                                                              aGCGCCAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCt                                          <  1:292475/122‑1 (MQ=255)                                                                                                   cAACGGCAAGCTGGATCGCAAAGCCTTGCCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAg                          <  2:507609/133‑1 (MQ=255)                                                                                                       ggCAAGCTGGCTCGCGAAGCCTTGCCGTTGCCAGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATg                      <  2:164640/133‑1 (MQ=255)                                                                                                                           cTTGCCGTTGCCTGATCTGAAGGCCCAAGCGCTAGGGCGTGAGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCGTTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTcgcg  <  2:165236/133‑1 (MQ=255)                                                                                                                              |                                                                                                                                  TTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCG  >  NC_000913/616905‑617159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑